PIII-25The functional consequence of the Glu298Asp polymorphism of the eNOS gene in humans

Nitric oxide inhibits adhesion of thrombocytes, proliferation and migration of smooth muscle cells and restricts oxidation of atherogenic low-density lipoproteins. Therefore, decreased production or activity of NO may play a role in the initiation, progression or complications of atherosclerosis. The aim of this study was to estimate the effect of Glu298Asp eNOS gene polymorphism on the individual risk for development of complicated carotid atherosclerotic plaque in patients from Serbia with advanced carotid atherosclerosis (CA) who had undergone endarterectomy. The study population included 233 patients. eNOS G894T gene polymorphism was identified by PCR and RFLP methods. Multivariate logistic regression analysis showed that Asp298Asp is an independent risk factor for the presence of complicated plaques in CA patients. Patients who were homozygous for the Asp298 allele had an adjusted OR of 4.36 for the development of complicated plaques compared to those that carry the Glu298 allele. Further validation and replication studies are needed.

Download Full-text

Prevalence of common polymorphisms of AT-rich interaction domain 1A and endothelial nitric oxide synthase in patients with endometriosis compared to control group

Journal of Endometriosis and Pelvic Pain Disorders ◽

10.1177/2284026518764352 ◽

2018 ◽

Vol 10 (1) ◽

pp. 26-31 ◽

Cited By ~ 1

Author(s):

Zohreh Tavana ◽

Azadeh Khalili ◽

Golnaz Namazi ◽

Ahmad Ebrahimi ◽

Sara Davoodi ◽

...

Keyword(s):

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Endothelial Nitric Oxide Synthase ◽

Control Group ◽

Enos Gene ◽

Interaction Domain ◽

Oxide Synthase ◽

Endothelial Nitric Oxide ◽

Rich Interaction ◽

Glu298asp Polymorphism

Introduction: Endometriosis is a common gynecologic disorder defined as ectopic presence of endometrial tissue in extrauterine sites. Endometriosis is associated with infertility and risk of malignancy. Identification of genetic factors responsible for development and malignant transformation of endometriosis can improve therapeutic approaches. In this study, we investigated the association of AT-rich interaction domain 1A ( ARID1A) and endothelial nitric oxide synthase ( eNOS) polymorphisms with endometriosis and staging of the disease. Methods: A total of 100 women with laparoscopy-confirmed diagnosis of endometriosis were included and compared with 100 women without endometriosis as the control group. Genotypes of patients regarding Gln920Ter polymorphism of ARID1A gene and Glu298Asp polymorphism of eNOS gene were determined by polymerase chain reaction techniques on blood samples from the study population. The prevalence of each genotype in endometriosis patients was compared with healthy controls using the chi-square test. Results: Significantly higher prevalence of non-CC genotype for ARID1A Gln920Ter polymorphism and non-GG genotype for G894T polymorphism of the eNOS gene was detected in the endometriosis group. There was no significant relationship between these polymorphisms and staging of endometriosis. Discussion: Significant variation of prevalence of Gln920Ter polymorphism of the ARID1A gene and Glu298Asp polymorphism of the eNOS gene among the two groups can indicate a causative effect of these genetic alterations on the development of endometriosis.

Download Full-text

Association of the Glu298Asp polymorphism of the eNOS Gene with ischemic heart disease in Japanese diabetic subjects

Diabetes Research and Clinical Practice ◽

10.1016/j.diabres.2007.12.019 ◽

2008 ◽

Vol 80 (2) ◽

pp. 275-279 ◽

Cited By ~ 24

Author(s):

Hiroyuki Tamemoto ◽

San-e Ishikawa ◽

Masanobu Kawakami

Keyword(s):

Heart Disease ◽

Ischemic Heart Disease ◽

Ischemic Heart ◽

Enos Gene ◽

Glu298asp Polymorphism

Download Full-text

Endothelial Nitric Oxide Synthase Glu298Asp Polymorphism as a Risk Factor for Prostate Cancer

The International Journal of Biological Markers ◽

10.5301/jbm.2012.9585 ◽

2013 ◽

Vol 28 (1) ◽

pp. 43-48 ◽

Cited By ~ 12

Author(s):

Seyed Amir Mohsen Ziaei ◽

Mohammad Samzadeh ◽

Seyed Hamid Jamaldini ◽

Mahdi Afshari ◽

Ali Akbar Haghdoost ◽

...

Keyword(s):

Prostate Cancer ◽

Nitric Oxide ◽

Nitric Oxide Synthase ◽

Endothelial Nitric Oxide Synthase ◽

Enos Gene ◽

Inverse Association ◽

Prostate Hyperplasia ◽

Oxide Synthase ◽

Endothelial Nitric Oxide ◽

Glu298asp Polymorphism

Background The endothelial form of nitric oxide synthases (eNOS) seems to have an important role in vascular development, maintenance of the vascular tone and tumor growth in human prostate cancer (PC). The purpose of this study was to investigate the association between grade and stage of disease, age of diagnosis, vascular or perineural invasion, pre-diagnostic plasma prostate-specific antigen (PSA) levels, prostate cancer risk and Glu298Asp polymorphism of the eNOS gene. Methods Ninety-five prostate cancer patients and 111 benign prostate hyperplasia subjects were included. The Glu298Asp polymorphism of the eNOS gene was determined by polymerase chain reaction and restriction fragment length polymorphism Results The odds ratio (OR) between the GT and GG polymorphism was 0.76, indicating that the presence of the GT polymorphism decreased the risk of prostate cancer of more than 20% compared to the GG polymorphism. This difference, however, was not statistically significant. The GT polymorphism had an inverse association with cancer grade compared to the reference group (OR=0.47, p value=0.2). Conclusions These results suggest that prostate cancer development is not associated with the Glu298Asp polymorphism of the endothelial nitric oxide synthase gene in our population. Further studies in larger samples are needed to confirm our results and characterize the molecular mechanisms by which eNOS is involved in the susceptibility to prostate cancer.

Download Full-text