Competitive adsorption of bovine serum albumin and lysozyme on a beta-tricalcium phosphate nanocoating

2019 ◽  
Vol 582 ◽  
pp. 123860 ◽  
Author(s):  
Lanxiang Ma ◽  
Dongwen Pang ◽  
Chunlin Deng
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Tricalcium Phosphate ◽  
Bovine Serum ◽  
Competitive Adsorption ◽  
Beta Tricalcium Phosphate

scholarly journals Bovine Serum Albumin Adsorption onto Hydroxyapatite and Biphasic Calcium Phosphate Ceramic Granules

2016 ◽  
Vol 721 ◽  
pp. 197-201 ◽  
Author(s):  
Inga Jurgelane ◽  
Armands Buss ◽  
Dagnija Loca
Keyword(s):  
Calcium Phosphate ◽  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Adsorption Capacity ◽  
Bovine Serum ◽  
Biphasic Calcium Phosphate ◽  
Calcium Phosphate Ceramic ◽  
Beta Tricalcium Phosphate ◽  
Albumin Adsorption ◽  
Hydroxyapatite Formation

Calcium phosphate ceramics are one of the most studied biomaterials for bone substitution and regeneration. Bioactivity is one of the most important properties for these materials and it can be evaluated by adsorption of proteins and by hydroxyapatite formation on the surface in simulated body fluid (SBF). The aim of this work was to evaluate the bioactivity of ceramic granules with various hydroxyapatite (HAp) and beta tricalcium phosphate (β-TCP) ratios by adsorption of bovine serum albumin (BSA) in SBF and phosphate saline buffer (PBS) solution. The highest adsorption capacity in both solutions was observed for biphasic calcium phosphate sample with HAp/β-TCP ratio 50/50 but the lowest – for sample 10/90. The adsorption capacity of all samples was more than 2 times higher in SBF media than in PBS. The possibility of hydroxyapatite formation was evaluated by changes of Ca2+ concentration in SBF. After 5 days of incubation at 37oC all samples showed a decrease in Ca2+ concentration.

Improvement of an artificial test substrate technique for evaluation of em immunocytochemical labeling

1987 ◽  
Vol 45 ◽  
pp. 762-763
Author(s):  
G. D. Gagne ◽  
M. F. Miller
Keyword(s):  
Bovine Serum Albumin ◽  
Serum Albumin ◽  
Bovine Serum ◽  
Artificial Substrate ◽  
Chemical Fixation ◽  
As If

We recently described an artificial substrate system which could be used to optimize labeling parameters in EM immunocytochemistry (ICC). The system utilizes blocks of glutaraldehyde polymerized bovine serum albumin (BSA) into which an antigen is incorporated by a soaking procedure. The resulting antigen impregnated blocks can then be fixed and embedded as if they are pieces of tissue and the effects of fixation, embedding and other parameters on the ability of incorporated antigen to be immunocyto-chemically labeled can then be assessed. In developing this system further, we discovered that the BSA substrate can also be dried and then sectioned for immunolabeling with or without prior chemical fixation and without exposing the antigen to embedding reagents. The effects of fixation and embedding protocols can thus be evaluated separately.

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