Biogenic synthesis of AuPd nanocluster as a peroxidase mimic and its application for colorimetric assay of acid phosphatase

2020 ◽  
Vol 589 ◽  
pp. 124444 ◽  
Author(s):  
Shanshan Zheng ◽  
Hongzhi Gu ◽  
Danyang Yin ◽  
Jinli Zhang ◽  
Wei Li ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Colorimetric Assay ◽  
Peroxidase Mimic ◽  
Biogenic Synthesis

scholarly journals Acid phosphatase activity in mononuclear phagocytes and the U937 cell line: monocyte-derived macrophages express tartrate-resistant acid phosphatase

Blood ◽  
1986 ◽  
Vol 67 (3) ◽  
pp. 729-734
Author(s):  
RG Snipes ◽  
KW Lam ◽  
RC Dodd ◽  
TK Gray ◽  
MS Cohen
Keyword(s):  
Acid Phosphatase ◽  
Cell Line ◽  
Gel Electrophoresis ◽  
U937 Cell ◽  
Colorimetric Assay ◽  
Mononuclear Phagocytes ◽  
Tartrate Resistant Acid Phosphatase ◽  
U937 Cells ◽  
Phagocytic Cells ◽  
The Relationship

Tartrate-resistant acid phosphatase (TRAcP) is used as a marker for osteoclasts, which are believed to be derived from phagocytic cells or phagocyte stem cell precursors. To further investigate the relationship between monocytic phagocytes and osteoclasts, acid phosphatase (AcP) activity was measured by three different techniques in human peripheral blood monocytes, monocyte-derived macrophages, and the U937 cell line. We found that cytochemistry and gel electrophoresis led to similar results, but that the colorimetric assay was inconsistent. Normal human peripheral monocytes expressed both tartrate-sensitive and -resistant AcP. In culture these cells formed polykaryons and expressed TRAcP activity that was further identified as an isoenzyme associated with bone tissue. In contrast, the U937 cells did not express TRAcP activity as measured by gel electrophoresis. Both U937 cells and monocytes possess material that interferes with interpretation of the colorimetric assay of AcP. The presence of TRAcP in monocyte-derived macrophages further supports the relationship between phagocytic cells and bone osteoclasts.

Fe-Porphyrin-Based Covalent Organic Framework As a Novel Peroxidase Mimic for a One-Pot Glucose Colorimetric Assay

2018 ◽  
Vol 1 (2) ◽  
pp. 382-388 ◽  
Author(s):  
Junning Wang ◽  
Xue Yang ◽  
Tianxiang Wei ◽  
Jianchun Bao ◽  
Qinshu Zhu ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Peroxidase Mimic ◽  
One Pot ◽  
Covalent Organic Framework ◽  
Organic Framework

scholarly journals Acid phosphatase activity in mononuclear phagocytes and the U937 cell line: monocyte-derived macrophages express tartrate-resistant acid phosphatase

Blood ◽  
1986 ◽  
Vol 67 (3) ◽  
pp. 729-734 ◽  
Author(s):  
RG Snipes ◽  
KW Lam ◽  
RC Dodd ◽  
TK Gray ◽  
MS Cohen
Keyword(s):  
Acid Phosphatase ◽  
Cell Line ◽  
Gel Electrophoresis ◽  
U937 Cell ◽  
Colorimetric Assay ◽  
Mononuclear Phagocytes ◽  
Tartrate Resistant Acid Phosphatase ◽  
U937 Cells ◽  
Phagocytic Cells ◽  
The Relationship

Abstract Tartrate-resistant acid phosphatase (TRAcP) is used as a marker for osteoclasts, which are believed to be derived from phagocytic cells or phagocyte stem cell precursors. To further investigate the relationship between monocytic phagocytes and osteoclasts, acid phosphatase (AcP) activity was measured by three different techniques in human peripheral blood monocytes, monocyte-derived macrophages, and the U937 cell line. We found that cytochemistry and gel electrophoresis led to similar results, but that the colorimetric assay was inconsistent. Normal human peripheral monocytes expressed both tartrate-sensitive and -resistant AcP. In culture these cells formed polykaryons and expressed TRAcP activity that was further identified as an isoenzyme associated with bone tissue. In contrast, the U937 cells did not express TRAcP activity as measured by gel electrophoresis. Both U937 cells and monocytes possess material that interferes with interpretation of the colorimetric assay of AcP. The presence of TRAcP in monocyte-derived macrophages further supports the relationship between phagocytic cells and bone osteoclasts.

scholarly journals Reverse-Bumpy-Ball-Type-Nanoreactor-Loaded Nylon Membranes as Peroxidase-Mimic Membrane Reactors for a Colorimetric Assay for H2O2

Sensors ◽  
2016 ◽  
Vol 16 (4) ◽  
pp. 465 ◽  
Author(s):  
Ying Tong ◽  
Xiangyu Jiao ◽  
Hankun Yang ◽  
Yongqiang Wen ◽  
Lei Su ◽  
...  
Keyword(s):  
Colorimetric Assay ◽  
Membrane Reactors ◽  
Peroxidase Mimic

scholarly journals Effect of Semen on Vaginal Fluid Cytokines and Secretory Leukocyte Protease Inhibitor

2008 ◽  
Vol 2008 ◽  
pp. 1-4 ◽  
Author(s):  
Kathy J. Agnew ◽  
Jan Aura ◽  
Norma Nunez ◽  
Zandra Lee ◽  
Rick Lawler ◽  
...  
Keyword(s):  
Acid Phosphatase ◽  
Protease Inhibitor ◽  
Proinflammatory Cytokine ◽  
Colorimetric Assay ◽  
Interleukin 1 ◽  
Secretory Leukocyte Protease Inhibitor ◽  
Vaginal Fluid ◽  
Gram Stain ◽  
Acid Phosphatase Spot Test ◽  
Cytokine Measurement

The presence of semen in vaginal fluid, as identified by an acid phosphatase spot test, does not influence vaginal proinflammatory cytokine concentrations.Objective: determine whether semen, as detected by acid phosphatase, influences vaginal cytokines or secretory leukocyte protease inhibitor concentrations.Methods: 138 pregnant women had vaginal fluid collected for Gram stain, acid phosphatase detection by colorimetric assay, and interleukin 1-Beta, interleukin-6, interleukin-8, and secretory leukocyte protease inhibitor measurement by enzyme immunoassay. Results for women with and without acid phosphatase were compared by Mann-Whitney test.Results: of 138 subjects, 28 (20%) had acid phosphatase detected; of these, only 19 (68%) reported recent intercourse and 3 (11%) had sperm seen on Gram stain. There were no significant differences in proinflammatory cytokine concentrations; however, secretory leukocyte protease inhibitor concentrations were significantly higher among women with acid phosphatase.Conclusions: proinflammatory cytokine measurement does not appear to be affected by the presence of semen, but secretory leukocyte protease inhibitor is significantly higher when semen is present. Detection of semen by acid phosphatase was associated with higher vaginal SLPI concentrations, however, the presence of semen did not appear to influence vaginal proinflammatory cytokine concentrations.

scholarly journals Core-Shell Magnetic Fe3O4/CNC@MOF Composites with Peroxidase-Like Activity for Colorimetric Detection of Phenol

2021 ◽  
Author(s):  
Chen Hou ◽  
Linhui Fu ◽  
Yang Wang ◽  
Wenqiang Chen ◽  
Fang Chen ◽  
...  
Keyword(s):  
Catalytic Mechanism ◽  
Colorimetric Assay ◽  
Colorimetric Detection ◽  
Core Shell ◽  
Peroxidase Mimic ◽  
Experimental Conditions ◽  
Fluorescence Test ◽  
Global Concern ◽  
Phenolic Wastewater ◽  
Excellent Stability

Abstract Rapid and accurate detection of phenolic wastewater from industries has created global concern. Herein, core-shell magnetic cellulose nanocrystals supported MOF (Fe3O4/CNC@ZIF-8) with robust peroxidase-like activity was synthesized with tannic acid as modifier and bridge. The peroxidase-mimic catalytic activity of as-prepared Fe3O4/CNC@ZIF-8 was further investigated using o-phenylenediamine (OPD) as peroxidase substrates in the presence of H2O2. Moreover, the experimental conditions were optimized and the kinetic analysis results showed that Fe3O4/CNC@ZIF-8 had higher affinity towards both the substrate OPD and H2O2 than horseradish peroxidase (HRP). Finally, a phenol colorimetric assay with a linear range of 2-200 µM and a detection limit of 0.316 µM was constructed. The catalytic mechanism of Fe3O4/CNC@ZIF-8 with phenol was further investigated by fluorescence test and the generated •OH was proved to act a crucial role to produce quinoid radicals. Additionally, the synthesized magnetic material had excellent stability and recyclability and ease to separation. These results suggest that the Fe3O4/CNC@ZIF-8 may be one of the promising candidates as peroxidase mimic for colorimetric detection of phenol.

Erythrophagocytosis in the Liver in Hemolytic Anemias

1968 ◽  
Vol 26 ◽  
pp. 184-185
Author(s):  
O. T. Minick ◽  
E. Orfei ◽  
F. Volini ◽  
G. Kent
Keyword(s):  
Acid Phosphatase ◽  
Oxidative Damage ◽  
Phosphatase Activity ◽  
Kupffer Cells ◽  
Acid Phosphatase Activity ◽  
Common Type ◽  
Red Cell ◽  
Cell Fragments ◽  
Reticulo Endothelial System ◽  
Important Site

Hemolytic anemias were produced in rats by administering phenylhydrazine or anti-erythrocytic (rooster) serum, the latter having agglutinin and hemolysin titers exceeding 1:1000.Following administration of phenylhydrazine, the erythrocytes undergo oxidative damage and are removed from the circulation by the cells of the reticulo-endothelial system, predominantly by the spleen. With increasing dosage or if animals are splenectomized, the Kupffer cells become an important site of sequestration and are greatly hypertrophied. Whole red cells are the most common type engulfed; they are broken down in digestive vacuoles, as shown by the presence of acid phosphatase activity (Fig. 1). Heinz body material and membranes persist longer than native hemoglobin. With larger doses of phenylhydrazine, erythrocytes undergo intravascular fragmentation, and the particles phagocytized are now mainly red cell fragments of varying sizes (Fig. 2).

The Demonstration of Human Prostatic Acid Phosphatase (Pap) With Phosphorylcholine

1976 ◽  
Vol 34 ◽  
pp. 88-89
Author(s):  
José A. Serrano ◽  
Hannah L. Wasserkrug ◽  
Anna A. Serrano ◽  
Arnold M. Seligman
Keyword(s):  
Acid Phosphatase ◽  
Prostate Gland ◽  
Prostatic Acid Phosphatase ◽  
Human Prostate ◽  
Rat Tissues ◽  
Specific Substrate ◽  
Acid Phosphatases ◽  
Lysosomal Acid ◽  
Cacodylate Buffer

As previously reported (1, 2) phosphorylcholine (PC) is a specific substrate for prostatatic acid phosphatase (PAP) as opposed to other acid phosphatases, e.g., lysosomal acid phosphatase. The specificity of PC for PAP is due to the pentavalent nitrogen in PC, a feature that renders PC resistant to hydrolysis by all other acid phosphatases. Detailed comparative cytochemical results in rat tissues are in press. This report deals with ultracytochemical results applying the method to normal and pathological human prostate gland.Fresh human prostate was obtained from 7 patients having transurethral resections or radical prostatectomies. The tissue was fixed in 3% glutaraldehyde- 0.1 M cacodylate buffer (pH 7.4) for 15 min, sectioned at 50 μm on a Sorvall TC-2 tissue sectioner, refixed for a total of 2 hr, and rinsed overnight in 0.1 M cacodylate buffer (pH 7.4)-7.5% sucrose.

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