Silver nanoparticles synthesized from Adenium obesum leaf extract induced DNA damage, apoptosis and autophagy via generation of reactive oxygen species

2016 ◽  
Vol 141 ◽  
pp. 158-169 ◽  
Author(s):  
Mohammad Abul Farah ◽  
Mohammad Ajmal Ali ◽  
Shen-Ming Chen ◽  
Ying Li ◽  
Fahad Mohammad Al-Hemaid ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Salacia mulbarica leaf extract mediated synthesis of silver nanoparticles for antibacterial and ct‐DNA damage via releasing of reactive oxygen species

2020 ◽  
Vol 14 (6) ◽  
pp. 485-490
Author(s):  
Chandra Sekhar Espenti ◽  
Kummari Subba Venkata Krishna Rao ◽  
Palakurthi Ramesh ◽  
Akila Chandra Sekhar ◽  
Kummara Madhusudana Rao
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Leaf Extract ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Ct Dna

scholarly journals Reactive Oxygen Species-Mediated Cytotoxicity in Liver Carcinoma Cells Induced by Silver Nanoparticles Biosynthesized Using Schinus molle Extract

Nanomaterials ◽  
2022 ◽  
Vol 12 (1) ◽  
pp. 161
Author(s):  
Waleed Ali Hailan ◽  
Khalid Mashay Al-Anazi ◽  
Mohammad Abul Farah ◽  
Mohammad Ajmal Ali ◽  
Ahmed Ali Al-Kawmani ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Dna Damage ◽  
Silver Nanoparticles ◽  
Reactive Oxygen Species Generation ◽  
Reactive Oxygen ◽  
Liver Carcinoma ◽  
Dependent Manner ◽  
Oxygen Species ◽  
Schinus Molle ◽  
Biosynthesized Agnps

Hepatocellular carcinoma (HCC) is the most common primary liver malignancy and is ranked as the third most common cause of cancer-related mortality worldwide. Schinus molle (S. mole) L. is an important medicinal plant that contains many bioactive compounds with pharmacological properties. The role of S. molle leaf extract in the biosynthesis of silver nanoparticles (AgNPs) was determined. The biosynthesized AgNPs were thoroughly characterized by UV–vis spectrophotometry, transmission electron microscopy (TEM), X-ray diffraction (XRD), and dynamic light scattering (DLS) techniques. Furthermore, the cytotoxic effect of the biosynthesized AgNPs using S. molle (SMAgNPs) against HepG2 liver cancer cells was investigated. Reactive oxygen species generation, apoptosis induction, DNA damage, and autophagy activity were analyzed. The results clearly showed that the biosynthesized silver nanoparticles inhibited the proliferation of HepG2 by significantly (p < 0.05) inducing oxidative stress, cytotoxicity, DNA damage, apoptosis, and autophagy in a dose- and time-dependent manner. These findings may encourage integrating the potential of natural products and the efficiency of silver nanoparticles for the fabrication of safe, environmentally friendly, and effective anticancer agents.

Novel Tetrazoles against Acanthamoeba castellanii Belonging to the T4 Genotype

Chemotherapy ◽  
2021 ◽  
Author(s):  
Yassmin Isse Wehelie ◽  
Naveed Ahmed Khan ◽  
Itrat Fatima ◽  
Areeba Anwar ◽  
Kanwal Kanwal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Silver Nanoparticles ◽  
Reactive Oxygen Species Generation ◽  
Acanthamoeba Castellanii ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Species Determination ◽  
One Pot ◽  
Tetrazole Derivatives

Background: Acanthamoeba castellanii is a pathogenic free-living amoeba responsible for blinding keratitis and fatal granulomatous amoebic encephalitis. However, treatments are not standardized but can involve the use of amidines, biguanides, and azoles. Objectives: The aim of this study was to synthesize a variety of synthetic tetrazole derivatives and test their activities against A. castellanii. Methods: A series of novel tetrazole compounds were synthesized by one-pot method and characterized by NMR and mass spectroscopy. These compounds were subjected to amoebicidal, and cytotoxicity assays against A. castellanii belonging to the T4 genotype and human keratinocyte skin cells respectively. Additionally, reactive oxygen species determination and electron microscopy studies were carried out. Furthermore, two of the seven compounds were conjugated with silver nanoparticles to study their antiamoebic potential. Results: A series of seven tetrazole derivatives were synthesized successfully. The selected tetrazoles showed anti-amoebic activities at 10µM concentration against A. castellanii in vitro. The compounds tested caused increased reactive oxygen species generation in A castellanii, and significant morphological damage to amoebal membranes. Moreover, conjugation of silver nanoparticles enhanced antiamoebic effects of two tetrazoles. Conclusions: The results showed that azole compounds hold promise in the development of new formulations of anti-Acanthamoebic agents.

Lifetime bioaccumulation of silver nanoparticles accelerates functional aging by inactivating antioxidant pathways, an effect reversed by pterostilbene

2021 ◽  
Author(s):  
Zi-Yu Chen ◽  
Yu-Chen Su ◽  
Fong-Yu Cheng ◽  
Shian-Jang Yan ◽  
Ying-Jan Wang
Keyword(s):  
Reactive Oxygen Species ◽  
Silver Nanoparticles ◽  
Adverse Effects ◽  
Signaling Pathways ◽  
Reactive Oxygen ◽  
Engineered Nanoparticles ◽  
Stress Signaling ◽  
Oxygen Species ◽  
Safety Concerns

Engineered nanoparticles raise safety concerns. Silver nanoparticles (AgNPs) exert acute and chronic adverse effects by inducing reactive oxygen species (ROS)-mediated stress signaling pathways. We investigated the mechanisms by which AgNPs...

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