Iontophoretic transdermal delivery using chitosan-coated PLGA nanoparticles for positively charged drugs

2017 ◽  
Vol 160 ◽  
pp. 520-526 ◽  
Author(s):  
Issei Takeuchi ◽  
Tomoyoshi Takeshita ◽  
Takaaki Suzuki ◽  
Kimiko Makino
Keyword(s):  
Transdermal Delivery ◽  
Plga Nanoparticles ◽  
Positively Charged

scholarly journals Layer-by-Layer 3D Constructs of Fibroblasts in Hydrogel for Examining Transdermal Penetration Capability of Nanoparticles

2016 ◽  
Vol 22 (4) ◽  
pp. 447-453 ◽  
Author(s):  
Xiaochun Hou ◽  
Shiying Liu ◽  
Min Wang ◽  
Christian Wiraja ◽  
Wei Huang ◽  
...  
Keyword(s):  
Transdermal Delivery ◽  
Three Dimensional ◽  
Layer By Layer ◽  
Surface Charges ◽  
Skin Model ◽  
Collagen Hydrogel ◽  
Penetration Ability ◽  
Charged Nanoparticles ◽  
Transdermal Penetration ◽  
Positively Charged

Nanoparticles are emerging transdermal delivery systems. Their size and surface properties determine their efficacy and efficiency to penetrate through the skin layers. This work utilizes three-dimensional (3D) bioprinting technology to generate a simplified artificial skin model to rapidly screen nanoparticles for their transdermal penetration ability. Specifically, this model is built through layer-by-layer alternate printing of blank collagen hydrogel and fibroblasts. Through controlling valve on-time, the spacing between printing lines could be accurately tuned, which could enable modulation of cell infiltration in the future. To confirm the effectiveness of this platform, a 3D construct with one layer of fibroblasts sandwiched between two layers of collagen hydrogel is used to screen silica nanoparticles with different surface charges for their penetration ability, with positively charged nanoparticles demonstrating deeper penetration, consistent with the observation from an existing study involving living skin tissue.

scholarly journals Comparative assessment of poly (D,L-lactide-co-glycolide) nanoparticles modified by either cetyltrimethylammonium bromide or chitosan for plasmid DNA adsorption

2017 ◽  
Vol 16 (10) ◽  
pp. 2315-2324
Author(s):  
Abd Almonem Doolaanea ◽  
Nur ‘Izzati Mansor ◽  
Nurul Hafizah Mohd Nor ◽  
Mohd Affendi Bin Mohd Shafri ◽  
Susi Sukmasari ◽  
...  
Keyword(s):  
Plasmid Dna ◽  
Plga Nanoparticles ◽  
Cell Uptake ◽  
Diffusion Method ◽  
Ammonium Bromide ◽  
Scanning Calorimetry ◽  
Cetyltrimethyl Ammonium Bromide ◽  
Dna Adsorption ◽  
Cetyltrimethyl Ammonium ◽  
Positively Charged

Purpose: To evaluate poly (D,L-lactide-co-glycolide) PLGA nanoparticles modified by cetyltrimethyl ammonium bromide (CTAB) or chitosan for plasmid DNA adsorption.Methods: PLGA nanoparticles were prepared by solvent diffusion method and modified by including CTAB in the aqueous (F1) or oil phase (F2), or by including low (F3) or medium (F4) molecular weight chitosan. The nanoparticles were  characterised by differential scanning calorimetry (DSC) and Fourier transform infrared spectroscopy (FTIR), as well as for cell toxicity, cell uptake and  transfection.Results: CTAB failed to confer positive charge on the nanoparticles. CTAB desorbed easily from F1 surface. This resulted in negative zeta potential, increased  cytotoxicity as well as decreased cell uptake and transfection. In F2, CTAB was located mainly in PLGA matrix, resulting in negative charge with decreased cytotoxicity, and increased cell uptake and transfection compared to F1. On the other hand, chitosan-modified nanoparticles (F3 and F4) showed stronger interaction between chitosan and PLGA, leading to positively-charged particles, decreased cytotoxicity, as well as increased cell uptake and transfection. Amongst the four formulations, F4 exhibited the highest transfection.Conclusion: These results should aid in understanding how PLGA nanoparticles are modified by CTAB and chitosan. Modification with chitosan yields PLGA  nanoparticles with higher DNA adsorption and transfection with lower cytotoxicity.Keywords: Chitosan, cetyltrimethyl ammonium bromide (CTAB), Nanoparticle, Poly (D,L-lactide-coglycolide) PLGA, Plasmid DNA adsorption, Gene therapy

Transdermal delivery of estradiol-loaded PLGA nanoparticles using iontophoresis for treatment of osteoporosis

2016 ◽  
Vol 27 (5) ◽  
pp. 475-483 ◽  
Author(s):  
Issei Takeuchi ◽  
Kohta Fukuda ◽  
Shiori Kobayashi ◽  
Kimiko Makino
Keyword(s):  
Transdermal Delivery ◽  
Plga Nanoparticles ◽  
Treatment Of Osteoporosis

Enhanced transdermal delivery of indomethacin using combination of PLGA nanoparticles and iontophoresis in vivo

2012 ◽  
Vol 92 ◽  
pp. 50-54 ◽  
Author(s):  
Keishiro Tomoda ◽  
Hiroto Terashima ◽  
Kenichi Suzuki ◽  
Toshio Inagi ◽  
Hiroshi Terada ◽  
...  
Keyword(s):  
Transdermal Delivery ◽  
Plga Nanoparticles

Hydrophilic drug-loaded PLGA nanoparticles for transdermal delivery

2017 ◽  
Vol 295 (6) ◽  
pp. 977-983 ◽  
Author(s):  
Issei Takeuchi ◽  
Keishiro Tomoda ◽  
Megumi Koji ◽  
Kimiko Makino
Keyword(s):  
Transdermal Delivery ◽  
Plga Nanoparticles ◽  
Hydrophilic Drug

Enhanced transdermal delivery of indomethacin-loaded PLGA nanoparticles by iontophoresis

2011 ◽  
Vol 88 (2) ◽  
pp. 706-710 ◽  
Author(s):  
Keishiro Tomoda ◽  
Hiroto Terashima ◽  
Kenichi Suzuki ◽  
Toshio Inagi ◽  
Hiroshi Terada ◽  
...  
Keyword(s):  
Transdermal Delivery ◽  
Plga Nanoparticles

scholarly journals Transdermal delivery of Minoxidil using HA-PLGA nanoparticles for the treatment in alopecia

2019 ◽  
Vol 23 (1) ◽  
Author(s):  
Woo Yeup Jeong ◽  
Sodam Kim ◽  
So Yun Lee ◽  
Hyeseon Lee ◽  
Dong Wook Han ◽  
...  
Keyword(s):  
Transdermal Delivery ◽  
Transdermal Drug Delivery ◽  
Skin Permeation ◽  
Plga Nanoparticles ◽  
Follicle Cells ◽  
Common Disease ◽  
Solvent Evaporation Method ◽  
The World ◽  
Low Efficiency ◽  
Permeation Test

Abstract Background Alopecia has become a very common disease that many people around the world are suffered. Minoxidil (MXD) is the most well-known commercialized drug in its treatment. However, in the case of MXD administration, there are some problems with low efficiency of transdermal delivery and additional side effects. Method MXD and Rhodamine B (Rho B) are encapsulated in poly(Lactide-co-Glycolide) grafted hyaluronate nanoparticles (HA-PLGA/MXD NPs, HA-PLGA/Rho B NPs) which is prepared with W/O/W solvent evaporation method. After then, the investigation is carried out to confirm the feasibility of NPs in alopecia treatment. Results Both of HA-PLGA/MXD NPs and HA-PLGA/Rho B NPs are successfully prepared. In addition, it is confirmed that HA-PLGA NPs sufficiently delivered to cells without any significant cytotoxicity by cell viability, cellular uptake and skin permeation test. Conclusion Taken together, HA-PLGA NPs as a transdermal delivery carrier to hair follicle cells can be exploited to develop the efficient and effective platform of transdermal drug delivery for the treatment of various diseases.

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