Layer-by-Layer 3D Constructs of Fibroblasts in Hydrogel for Examining Transdermal Penetration Capability of Nanoparticles

Nanoparticles are emerging transdermal delivery systems. Their size and surface properties determine their efficacy and efficiency to penetrate through the skin layers. This work utilizes three-dimensional (3D) bioprinting technology to generate a simplified artificial skin model to rapidly screen nanoparticles for their transdermal penetration ability. Specifically, this model is built through layer-by-layer alternate printing of blank collagen hydrogel and fibroblasts. Through controlling valve on-time, the spacing between printing lines could be accurately tuned, which could enable modulation of cell infiltration in the future. To confirm the effectiveness of this platform, a 3D construct with one layer of fibroblasts sandwiched between two layers of collagen hydrogel is used to screen silica nanoparticles with different surface charges for their penetration ability, with positively charged nanoparticles demonstrating deeper penetration, consistent with the observation from an existing study involving living skin tissue.

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Cooperative Charging Effects of Fibers from Electrospinning of Electrically Dissimilar Polymers

International Nonwovens Journal ◽

10.1177/1558925004os-1300406 ◽

2004 ◽

Vol os-13 (4) ◽

pp. 1558925004os-13 ◽

Cited By ~ 3

Author(s):

Heidi L. Schreuder-Gibson ◽

Phil Gibson ◽

Peter Tsai

Keyword(s):

Pressure Drop ◽

Fiber Diameter ◽

Layer By Layer ◽

Surface Charges ◽

Positive Voltage ◽

Charge Decay ◽

Geometrical Factors ◽

Filtration Properties ◽

Positively Charged ◽

Electrical Charging

Electrical charging and residual charge decay of electro-spun nonwoven webs comprised of two electrically dissimilar polymers were studied in an effort to investigate their filtration properties. Polystyrene (PS) and polyacrylonitrile (PAN) were electrospun separately, in a layer-by-layer configuration and simultaneously in a side-by-side bi-component apparatus into thin webs on a polypropylene spunbond substrate. During electrospinning of the PS and PAN polymer solutions, the fibers became positively charged when positive voltage was applied to the solution-filled spinning nozzle and became negatively charged when negative voltage was applied. This study was undertaken to examine the effect of cooperative charging from electrospinning of the two polymers, the effect of the three types of web constructions on charge retention, and filtration properties of the fibers. It was found that single, multilayered, and bi-component webs retained surface charges in the thousands of volts that diminished very little over a 20-hour period, but eventually bled off while resting for three months. Filtration properties were found to be exceptionally high for some, but not all, electrospun samples; filtration was found to have a weak dependence on both surface charge and web geometrical factors, particularly the fiber diameter, that influence pressure drop of the aerosol test.

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3D Printing of High Viscosity Reinforced Silicone Elastomers

Polymers ◽

10.3390/polym13142239 ◽

2021 ◽

Vol 13 (14) ◽

pp. 2239

Author(s):

Nicholas Rodriguez ◽

Samantha Ruelas ◽

Jean-Baptiste Forien ◽

Nikola Dudukovic ◽

Josh DeOtte ◽

...

Keyword(s):

Mechanical Properties ◽

High Performance ◽

New Technologies ◽

Three Dimensional ◽

High Viscosity ◽

Layer By Layer ◽

Silicone Elastomers ◽

Uv Curable ◽

Octet Truss ◽

Tunable Mechanical Properties

Recent advances in additive manufacturing, specifically direct ink writing (DIW) and ink-jetting, have enabled the production of elastomeric silicone parts with deterministic control over the structure, shape, and mechanical properties. These new technologies offer rapid prototyping advantages and find applications in various fields, including biomedical devices, prosthetics, metamaterials, and soft robotics. Stereolithography (SLA) is a complementary approach with the ability to print with finer features and potentially higher throughput. However, all high-performance silicone elastomers are composites of polysiloxane networks reinforced with particulate filler, and consequently, silicone resins tend to have high viscosities (gel- or paste-like), which complicates or completely inhibits the layer-by-layer recoating process central to most SLA technologies. Herein, the design and build of a digital light projection SLA printer suitable for handling high-viscosity resins is demonstrated. Further, a series of UV-curable silicone resins with thiol-ene crosslinking and reinforced by a combination of fumed silica and MQ resins are also described. The resulting silicone elastomers are shown to have tunable mechanical properties, with 100–350% elongation and ultimate tensile strength from 1 to 2.5 MPa. Three-dimensional printed features of 0.4 mm were achieved, and complexity is demonstrated by octet-truss lattices that display negative stiffness.

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A guideline for 3D printing terminology in biomedical research utilizing ISO/ASTM standards

3D Printing in Medicine ◽

10.1186/s41205-021-00098-5 ◽

2021 ◽

Vol 7 (1) ◽

Author(s):

Amy E. Alexander ◽

Nicole Wake ◽

Leonid Chepelev ◽

Philipp Brantner ◽

Justin Ryan ◽

...

Keyword(s):

3D Printing ◽

Medical Literature ◽

Three Dimensional ◽

Physical Object ◽

Medical Community ◽

Layer By Layer ◽

Digital File ◽

Standardized Terminology ◽

Standard Terms ◽

The Impact

AbstractFirst patented in 1986, three-dimensional (3D) printing, also known as additive manufacturing or rapid prototyping, now encompasses a variety of distinct technology types where material is deposited, joined, or solidified layer by layer to create a physical object from a digital file. As 3D printing technologies continue to evolve, and as more manuscripts describing these technologies are published in the medical literature, it is imperative that standardized terminology for 3D printing is utilized. The purpose of this manuscript is to provide recommendations for standardized lexicons for 3D printing technologies described in the medical literature. For all 3D printing methods, standard general ISO/ASTM terms for 3D printing should be utilized. Additional, non-standard terms should be included to facilitate communication and reproducibility when the ISO/ASTM terms are insufficient in describing expository details. By aligning to these guidelines, the use of uniform terms for 3D printing and the associated technologies will lead to improved clarity and reproducibility of published work which will ultimately increase the impact of publications, facilitate quality improvement, and promote the dissemination and adoption of 3D printing in the medical community.

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Three-dimensionally printed polycaprolactone/multicomponent bioactive glass scaffolds for potential application in bone tissue engineering

Biomedical glasses ◽

10.1515/bglass-2020-0006 ◽

2020 ◽

Vol 6 (1) ◽

pp. 57-69

Author(s):

Amirhosein Fathi ◽

Farzad Kermani ◽

Aliasghar Behnamghader ◽

Sara Banijamali ◽

Masoud Mozafari ◽

...

Keyword(s):

Tissue Engineering ◽

3D Printing ◽

Bone Tissue Engineering ◽

Bone Tissue ◽

Three Dimensional ◽

Layer By Layer ◽

Composite Scaffolds ◽

3D Printed ◽

Co Doped

AbstractOver the last years, three-dimensional (3D) printing has been successfully applied to produce suitable substitutes for treating bone defects. In this work, 3D printed composite scaffolds of polycaprolactone (PCL) and strontium (Sr)- and cobalt (Co)-doped multi-component melt-derived bioactive glasses (BGs) were prepared for bone tissue engineering strategies. For this purpose, 30% of as-prepared BG particles (size <38 μm) were incorporated into PCL, and then the obtained composite mix was introduced into a 3D printing machine to fabricate layer-by-layer porous structures with the size of 12 × 12 × 2 mm3.The scaffolds were fully characterized through a series of physico-chemical and biological assays. Adding the BGs to PCL led to an improvement in the compressive strength of the fabricated scaffolds and increased their hydrophilicity. Furthermore, the PCL/BG scaffolds showed apatite-forming ability (i.e., bioactivity behavior) after being immersed in simulated body fluid (SBF). The in vitro cellular examinations revealed the cytocompatibility of the scaffolds and confirmed them as suitable substrates for the adhesion and proliferation of MG-63 osteosarcoma cells. In conclusion, 3D printed composite scaffolds made of PCL and Sr- and Co-doped BGs might be potentially-beneficial bone replacements, and the achieved results motivate further research on these materials.

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Anomalous Laterally Stressed Kinetically Trapped DNA Surface Conformations

Nano-Micro Letters ◽

10.1007/s40820-021-00626-2 ◽

2021 ◽

Vol 13 (1) ◽

Author(s):

Valery V. Prokhorov ◽

Nikolay A. Barinov ◽

Kirill A. Prusakov ◽

Evgeniy V. Dubrovin ◽

Maxim D. Frank-Kamenetskii ◽

...

Keyword(s):

Electrostatic Interaction ◽

Mechanical Response ◽

Persistence Length ◽

Strong Impact ◽

List Type ◽

Surface Charges ◽

Monomolecular Films ◽

Chain Approximation ◽

The Impact ◽

Positively Charged

Highlights DNA kinking is inevitable for the highly anisotropic 1D–1D electrostatic interaction with the one-dimensionally periodically charged surface. The double helical structure of the DNA kinetically trapped on positively charged monomolecular films comprising the lamellar templates is strongly laterally stressed and extremely perturbed at the nanometer scale. The DNA kinetic trapping is not a smooth 3D—> 2D conformational flattening but is a complex nonlinear in-plane mechanical response (bending, tensile and unzipping) driven by the physics beyond the scope of the applicability of the linear worm-like chain approximation. Abstract Up to now, the DNA molecule adsorbed on a surface was believed to always preserve its native structure. This belief implies a negligible contribution of lateral surface forces during and after DNA adsorption although their impact has never been elucidated. High-resolution atomic force microscopy was used to observe that stiff DNA molecules kinetically trapped on monomolecular films comprising one-dimensional periodically charged lamellar templates as a single layer or as a sublayer are oversaturated by sharp discontinuous kinks and can also be locally melted and supercoiled. We argue that kink/anti-kink pairs are induced by an overcritical lateral bending stress (> 30 pNnm) inevitable for the highly anisotropic 1D-1D electrostatic interaction of DNA and underlying rows of positive surface charges. In addition, the unexpected kink-inducing mechanical instability in the shape of the template-directed DNA confined between the positively charged lamellar sides is observed indicating the strong impact of helicity. The previously reported anomalously low values of the persistence length of the surface-adsorbed DNA are explained by the impact of the surface-induced low-scale bending. The sites of the local melting and supercoiling are convincingly introduced as other lateral stress-induced structural DNA anomalies by establishing a link with DNA high-force mechanics. The results open up the study in the completely unexplored area of the principally anomalous kinetically trapped DNA surface conformations in which the DNA local mechanical response to the surface-induced spatially modulated lateral electrostatic stress is essentially nonlinear. The underlying rich and complex in-plane nonlinear physics acts at the nanoscale beyond the scope of applicability of the worm-like chain approximation.

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Direct Patterning and Spontaneous Self-Assembly of Graphene Oxide via Electrohydrodynamic Jet Printing for Energy Storage and Sensing

Micromachines ◽

10.3390/mi11010013 ◽

2019 ◽

Vol 11 (1) ◽

pp. 13 ◽

Cited By ~ 2

Author(s):

Bin Zhang ◽

Jaehyun Lee ◽

Mincheol Kim ◽

Naeeung Lee ◽

Hyungdong Lee ◽

...

Keyword(s):

Graphene Oxide ◽

Energy Storage ◽

Self Assembly ◽

High Performance ◽

Three Dimensional ◽

Chemical Properties ◽

Layer By Layer ◽

Energy Storage Devices ◽

Laminar Structure ◽

Jet Printing

The macroscopic assembly of two-dimensional materials into a laminar structure has received considerable attention because it improves both the mechanical and chemical properties of the original materials. However, conventional manufacturing methods have certain limitations in that they require a high temperature process, use toxic solvents, and are considerably time consuming. Here, we present a new system for the self-assembly of layer-by-layer (LBL) graphene oxide (GO) via an electrohydrodynamic (EHD) jet printing technique. During printing, the orientation of GO flakes can be controlled by the velocity distribution of liquid jet and electric field-induced alignment spontaneously. Closely-packed GO patterns with an ordered laminar structure can be rapidly realized using an interfacial assembly process on the substrates. The surface roughness and electrical conductivity of the LBL structure were significantly improved compared with conventional dispensing methods. We further applied this technique to fabricate a reduced graphene oxide (r-GO)-based supercapacitor and a three-dimensional (3D) metallic grid hybrid ammonia sensor. We present the EHD-assisted assembly of laminar r-GO structures as a new platform for preparing high-performance energy storage devices and sensors.

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Multilayer composite beads constructed via layer-by-layer self-assembly for lysozyme controlled release

RSC Advances ◽

10.1039/c4ra02780a ◽

2014 ◽

Vol 4 (46) ◽

pp. 24369-24376 ◽

Cited By ~ 10

Author(s):

Jiemin Zhao ◽

Xiaoping Wang ◽

Yanshen Kuang ◽

Yufeng Zhang ◽

Xiaowen Shi ◽

...

Keyword(s):

Controlled Release ◽

Self Assembly ◽

Cross Linking ◽

Layer By Layer ◽

Multilayer Composite ◽

Composite Beads ◽

Assembly Technique ◽

Positively Charged

Alginate (ALG)–lysozyme (LZ) beads were fabricated by a cross-linking process. Negatively charged ALG and positively charged LZ were alternately deposited on the positively charged ALG–LZ beads via a layer-by-layer (LBL) self-assembly technique.

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Biological Multilayer Films Assembled by Redox Polymer and HRP on Screen-Printed Carbon Electrode

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.571.56 ◽

2012 ◽

Vol 571 ◽

pp. 56-59

Author(s):

Yu Fang Sha ◽

Mei Zhao ◽

Ming Quan Yang ◽

Hai Xin Bai ◽

Man Zhao

Keyword(s):

Horseradish Peroxidase ◽

Electrostatic Interaction ◽

Electrode Surface ◽

Multilayer Films ◽

Carbon Electrode ◽

Layer By Layer ◽

Redox Polymer ◽

Screen Printed Carbon Electrode ◽

Positively Charged ◽

Screen Printed

Biological multilayer films of redox polymer and horseradish peroxidase (HRP) were successfully assembled on a screen-printed carbon electrode using layer-by-layer (LBL) assembled method based on the electrostatic interaction. The screen-printed carbon electrode surface was modified by the positively charged redox polymer, and the negatively charged HRP by LBL method.

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Evaluation of Parts Produced by a Novel Additive Manufacturing Process

Applied Mechanics and Materials ◽

10.4028/www.scientific.net/amm.315.63 ◽

2013 ◽

Vol 315 ◽

pp. 63-67 ◽

Cited By ~ 1

Author(s):

Muhammad Fahad ◽

Neil Hopkinson

Keyword(s):

Additive Manufacturing ◽

Rapid Prototyping ◽

Manufacturing Process ◽

High Speed ◽

Three Dimensional ◽

Coordinate Measuring Machine ◽

Layer By Layer ◽

Nylon 12 ◽

Measuring Machine ◽

End Use

Rapid prototyping refers to building three dimensional parts in a tool-less, layer by layer manner using the CAD geometry of the part. Additive Manufacturing (AM) is the name given to the application of rapid prototyping technologies to produce functional, end use items. Since AM is relatively new area of manufacturing processes, various processes are being developed and analyzed for their performance (mainly speed and accuracy). This paper deals with the design of a new benchmark part to analyze the flatness of parts produced on High Speed Sintering (HSS) which is a novel Additive Manufacturing process and is currently being developed at Loughborough University. The designed benchmark part comprised of various features such as cubes, holes, cylinders, spheres and cones on a flat base and the build material used for these parts was nylon 12 powder. Flatness and curvature of the base of these parts were measured using a coordinate measuring machine (CMM) and the results are discussed in relation to the operating parameters of the process.The result show changes in the flatness of part with the depth of part in the bed which is attributed to the thermal gradient within the build envelope during build.

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