Penetration of Nile red-loaded nanostructured lipid carriers (NLCs) across the porcine cornea

2019 ◽  
Vol 176 ◽  
pp. 371-378 ◽  
Author(s):  
Pattravee Niamprem ◽  
Sangly P. Srinivas ◽  
Waree Tiyaboonchai
Keyword(s):  
Nile Red ◽  
Nanostructured Lipid Carriers ◽  
Porcine Cornea

En face dual fluorescence staining of fatty streaks allows observation of initiation and progression of atherosclerotic lesions

1995 ◽  
Vol 53 ◽  
pp. 864-865
Author(s):  
Anne M. Klinkner ◽  
Crystal R. Waites ◽  
Peter J. Bugelski ◽  
William D. Kerns
Keyword(s):  
Fluorescent Dyes ◽  
Nile Red ◽  
Dimethyl Formamide ◽  
High Cholesterol Diet ◽  
Methods Development ◽  
Atherosclerotic Lesions ◽  
En Face ◽  
Biochemical Evaluation ◽  
Stock Solutions ◽  
Dual Staining

A primary effort in the understanding of the progression of atherosclerotic disease has been methods development for visualization of the atherosclerotic plaque. We introduce a new method for the qualitative analysis of lipids in atherosclerotic fatty streaks which also retains those lipids for biochemical evaluation. An original aspect of the process is the ability to view an entire fatty streak en face, selectively stained for specific lipid classes within the lesion.New Zealand white rabbits were fed a high cholesterol diet(0.15%-0.3% for 14 wks). The aorta was removed and fixed in Carson's phosphate buffered formaldehyde followed by dual staining in the fluorescent dyes Nile red and filipin. Stock solutions of nile red(0.5mg/ml acetone) and filipin(2.5mg/ml dimethyl formamide) were prepared and kept at -20°C; all subsequent steps were at RT. 0.5cm × 1.0cm pieces of aorta were trimmed and adventitia removed. The pieces were then washed 3×15 min in PBS w/o CaMg, soaked in Nile red(NR)/filipin(Fl) stain(100(il NR stock + 200μl Fl stock in 10 ml PBS for 30 min, washed in PBS 3×30 min, rinsed with distilled water, mounted(Crystal Mount, Biomedia) and coverslipped and viewed by fluorescence microscopy.

The Nile-Red Sea Railroad

1907 ◽  
Vol 64 (1644supp) ◽  
pp. 8-9
Author(s):  
Harold J. Shepstone
Keyword(s):  
Red Sea ◽  
Nile Red

Decellularized Porcine Cornea-Based Hydrogels for Regeneration of Corneal Epithelium and Stroma

2020 ◽  
Author(s):  
Fuyan Wang ◽  
Weiyun Shi ◽  
Hua Li ◽  
Long Zhao ◽  
Dapeng Sun ◽  
...  
Keyword(s):  
Corneal Epithelium ◽  
Porcine Cornea

Targeted Nanostructured Lipid Carriers for Delivery of Paclitaxel to Cancer Cells: Preparation, Characterization, and Cell Toxicity

2017 ◽  
Vol 14 (8) ◽  
Author(s):  
Mahboubeh Rezazadeh ◽  
Jaber Emami ◽  
Farshid Hassanzadeh ◽  
Hojjat Sadeghi ◽  
Mahboubeh Rostami ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Nanostructured Lipid Carriers ◽  
Cell Toxicity

Solid Lipid Nanoparticles and Nanostructured Lipid Carriers: A Review

2011 ◽  
Vol 6 (4) ◽  
pp. 240-250 ◽  
Author(s):  
Rajashree Hirlekar ◽  
Harshal Garse ◽  
Vilasrao Kadam
Keyword(s):  
Solid Lipid Nanoparticles ◽  
Lipid Nanoparticles ◽  
Nanostructured Lipid Carriers ◽  
Solid Lipid

scholarly journals Optimization of Tilmicosin-Loaded Nanostructured Lipid Carriers Using Orthogonal Design for Overcoming Oral Administration Obstacle

Pharmaceutics ◽  
2021 ◽  
Vol 13 (3) ◽  
pp. 303
Author(s):  
Jia Wen ◽  
Xiuge Gao ◽  
Qian Zhang ◽  
Benazir Sahito ◽  
Hongbin Si ◽  
...  
Keyword(s):  
Oral Administration ◽  
Oral Delivery ◽  
Bacterial Infections ◽  
Cold Water ◽  
Orthogonal Design ◽  
Drug Loading ◽  
Absorption Efficiency ◽  
Nanostructured Lipid Carriers ◽  
Orthogonal Experimental Design ◽  
Hydrodynamic Diameter

Tilmicosin (TMS) is widely used to treat bacterial infections in veterinary medicine, but the clinical effect is limited by its poor solubility, bitterness, gastric instability, and intestinal efflux transport. Nanostructured lipid carriers (NLCs) are nowadays considered to be a promising vector of therapeutic drugs for oral administration. In this study, an orthogonal experimental design was applied for optimizing TMS-loaded NLCs (TMS-NLCs). The ratios of emulsifier to mixed lipids, stearic acid to oleic acid, drugs to mixed lipids, and cold water to hot emulsion were selected as the independent variables, while the hydrodynamic diameter (HD), drug loading (DL), and entrapment efficiency (EE) were the chosen responses. The optimized TMS-NLCs had a small HD, high DL, and EE of 276.85 ± 2.62 nm, 9.14 ± 0.04%, and 92.92 ± 0.42%, respectively. In addition, a low polydispersity index (0.231 ± 0.001) and high negative zeta potential (−31.10 ± 0.00 mV) indicated the excellent stability, which was further demonstrated by uniformly dispersed spherical nanoparticles under transmission electron microscopy. TMS-NLCs exhibited a slow and sustained release behavior in both simulated gastric juice and intestinal fluid. Furthermore, MDCK-chAbcg2/Abcb1 cell monolayers were successfully established to evaluate their absorption efficiency and potential mechanism. The results of biodirectional transport showed that TMS-NLCs could enhance the cellular uptake and inhibit the efflux function of drug transporters against TMS in MDCK-chAbcg2/Abcb1 cells. Moreover, the data revealed that TMS-NLCs could enter the cells mainly via the caveolae/lipid raft-mediated endocytosis and partially via macropinocytosis. Furthermore, TMS-NLCs showed the same antibacterial activity as free TMS. Taken together, the optimized NLCs were the promising oral delivery carrier for overcoming oral administration obstacle of TMS.

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