Determination of physiological features of mutant Trichoderma harzianum isolates and antagonistic activity against plant pathogen Fusarium sp

2011 ◽  
Vol 22 ◽  
pp. S142
Author(s):  
Meral Yilmaz ◽  
Merih Kivanc ◽  
Ahmet Ulker
Keyword(s):  
Plant Pathogen ◽  
Trichoderma Harzianum ◽  
Antagonistic Activity ◽  
Fusarium Sp

Biocontrol of seed-borne Alternaria raphani and A. brassicicola

1987 ◽  
Vol 33 (10) ◽  
pp. 850-856 ◽  
Author(s):  
G. Vannacci ◽  
G. E. Harman
Keyword(s):  
Biological Control ◽  
Host Range ◽  
Trichoderma Harzianum ◽  
Pythium Species ◽  
Biological Control Agents ◽  
Fusarium Sp ◽  
Chamber Studies ◽  
Growth Chamber Studies ◽  
Radish Seedlings

Forty-two microorganisms were tested as biological control agents against Alternaria raphani and A. brassicicola. Tests were conducted for in vitro antagonistic ability, for ability to control the pathogens on naturally infected seeds germinated on moistened blotters, and in planting mix in growth chamber studies, and for their ability to reduce pod infection. The organisms tested were obtained from cruciferous seeds or were strains already identified as being effective against soil-borne Pythium species. The blotter test indicated that six organisms increased both the number of healthy seedlings and the number of seedlings produced from A. raphani infected radish seeds. An additional seven strains improved either germination or increased the number of healthy seedlings. Twenty-nine organisms increased the number of healthy cabbage seedlings from A. brassicicola infected seeds, but total germination was not modified by any treatment. Experiments in planting mix showed that five antagonists (Chaetomium globosum, two strains of Trichoderma harzianum, T. koningii, and Fusarium sp.) increased the number of healthy plants in both radish samples tested, while four additional antagonists provided a significant increase in only one of the samples tested. The five antagonists that consistently increased numbers of healthy radish seedlings also decreased pod infection by A. raphani. None were as effective as iprodrone, however. Several effective antagonists were found to be mycoparasitic against Alternaria spp. Some strains of Trichoderma previously found to be effective against Pythium spp. were also effective against Alternaria spp., indicating that these strains have a wide host range.

scholarly journals Antifungal activity of several isolates of Trichoderma against Cladosporium and Botrytis

2018 ◽  
Vol 8 (1) ◽  
pp. 88-91 ◽  
Author(s):  
M. Skaptsov ◽  
S. Smirnov ◽  
M. Kutsev ◽  
O. Uvarova ◽  
T. Sinitsyna ◽  
...  
Keyword(s):  
Antifungal Activity ◽  
Trichoderma Harzianum ◽  
Its Region ◽  
Botanical Garden ◽  
Antagonistic Activity ◽  
Antagonistic Effect ◽  
Soil Samples ◽  
Morphological Observation ◽  
The South

<p><em>Trichoderma</em> isolates (SSBGT07, SSBGT08, SSBGT09, SSBGT10) were isolated from the soil samples of the South-Siberian Botanical Garden and identified using morphological observation and ITS region analysis as <em>Trichoderma harzianum</em>, <em>T. asperellum, T. ghanense</em>, and <em>T. longibranchiatum</em>. Antagonistic activity against <em>Cladosporium </em>sp. and<em> Botrytis </em>sp. was evaluated <em>in vitro</em>. All isolates showed antagonistic effect by competition against <em>Cladosporium </em>sp. <em>T. asperellum </em>and <em>T. longibranchiatum</em> showed antagonism against <em>Botrytis </em>sp. All isolates showed hyper sporulation on the sclerotia of <em>Botrytis</em> sp. (except the <em>T. ghanense</em>) and colonies of the <em>Cladosporium</em> sp. Our study provides new isolates that affect the <em>Cladosporium </em>sp. and<em> Botrytis </em>sp.</p>

scholarly journals In Vitro Inhibition of Fungal Antagonists on Fusarium sp., the Disease Causative Agent on Dragon Fruit Plant (Hylocereus undatus (Haw.) Britton & Rose)

2018 ◽  
Vol 5 (2) ◽  
pp. 224
Author(s):  
Dewa Ayu Andriastini ◽  
Yan Ramona ◽  
Meitini Wahyuni Proborini
Keyword(s):  
Causative Agent ◽  
Biocontrol Agent ◽  
Antagonistic Activity ◽  
Assay Method ◽  
In Vitro Growth ◽  
Dragon Fruit ◽  
Fusarium Sp ◽  
In Vitro Inhibition ◽  
Hylocereus Undatus

A research on in vitro inhibition of fungal antagonists, isolated from dragon fruit plantation in Sembung village, Bali, on Fusarium sp. (the disease causative agent of dragon fruit plant) was conducted with the main objective to investigate the effectiveness of these fungal antagonists to inhibit the in vitro growth of the pathogen. Dual assay method was applied in this experiment. The results showed that three potential fungal antagonists were successfully isolated in this research and they were identified as Trichoderma harzianum, Aspergillus niger, dan Paecilomyces lilacinus. All these fungal antagonists showed antagonistic activity against Fusarium sp. which was statistically significant (p<0.05) when compared to control. This indicated that all antagonist isolates were potential to be developed as biocontrol agent candidates.

scholarly journals Biocontrol potential of Trichoderma harzianum in controlling wilt disease of pistachio caused by Verticillium dahliae

2017 ◽  
Vol 57 (2) ◽  
pp. 185-193 ◽  
Author(s):  
Zeinab Fotoohiyan ◽  
Saeed Rezaee ◽  
Gholam Hosein Shahidi Bonjar ◽  
Amir Hossein Mohammadi ◽  
Mohammad Moradi
Keyword(s):  
Verticillium Dahliae ◽  
Trichoderma Harzianum ◽  
Pathogenic Fungus ◽  
Antagonistic Activity ◽  
Wilt Disease ◽  
Dual Culture ◽  
Volatile Metabolites ◽  
Biocontrol Potential

Abstract Verticillium wilt caused by Verticillium dahliae, is one of the most devastating diseases in pistachio orchards in the world including Iran. In search for an effective non-chemical strategy for the management of this disease, we evaluated the biocontrol potential of Trichoderma harzianum isolates obtained from the rhizosphere of healthy pistachio trees in different locations of the Kerman province of Iran against V. dahliae under laboratory and greenhouse conditions. Dual culture tests in the laboratory were conducted in a completely randomized design using 72 T. harzianum isolates. Twenty isolates showed the highest in vitro antagonistic activity. The results indicated that all 20 isolates were capable of inhibiting the mycelial growth of V. dahliae significantly. Among them, isolates Tr8 and Tr19 were the most effective by 88.89% and 85.12% inhibition, respectively. Extracted cell free metabolites of all effective isolates also inhibited the growth of V. dahliae in the culture medium significantly. According to the results, isolates Tr4 and Tr6 inhibited fungal pathogen growth by 94.94% and 88.15% respectively, through production of non-volatile metabolites. In the evaluation of volatile metabolites, isolates Tr5 and Tr4 were the most effective by 26.27% and 24.49% growth inhibition, respectively. Based on the results of the in vitro experiments, the five most effective isolates were selected for evaluation under greenhouse conditions for their biocontrol potential in controlling Verticillium wilt of pistachio. Results of the greenhouse, (in vivo) experiments were positive and indicated that the occurrence of wilt disease in plants treated with the antagonists alone or in combination with pathogenic fungus was lower than in plants inoculated with pathogen alone. The overall results of this study suggest that Trichoderma fungal antagonist may be an effective biocontrol agent for the control of Verticillium wilt of pistachio.

scholarly journals ACTIVITY OF ENZYMATIC CELLULOLYTIC COMPLEX AND ANTAGONISTIC PROPERTIES OF TRICHODERMA HARZIANUM 128

2021 ◽  
Vol 33 ◽  
pp. 13-24
Author(s):  
Dimova S. B. ◽  
Derkach S. M. ◽  
Volkohon V. V.
Keyword(s):  
High Activity ◽  
Trichoderma Harzianum ◽  
Biochemical Study ◽  
Antagonistic Activity ◽  
Chicken Manure ◽  
Cellulolytic Enzymes ◽  
Carbon And Nitrogen ◽  
Activity Of Enzymes ◽  
Production Inspection

Objective. To study the activity of enzymes of the cellulolytic complex of the association of micromycetes Trichoderma harzianum 128 and their antagonistic activity; to study the possibility of the influence of fungal introduction to the composted substrate on the composting rate and quality of composts. Methods. Microbiological (for growing micromycetes in digest media, accounting the number of T. harzianum 128 and determining the antagonistic activity of the association), agrochemical (to determine the characteristics of composting chicken manure-based substrates and content of carbon and nitrogen in these substrates), biochemical (study of activity of cellulolytic enzymes), production inspection (on the basis of Agrofirma KOLOS LLC, Region of Kyiv). Results. The association of T. harzianum 128 micromycetes is characterized by high activity of exoglucanase, endoglucanase and β-glucosidase, which contributes to reduction in the composting time of organic matter upon the introduction of fungi to the chicken manure-based substrate. T. harzianum 128 has pronounced antifungal properties against pathogens of certain diseases of crops (Fusarium oxysporum, F. sulfurum, Nigrospora oryzae). Enrichment of composted substrates with the studied micromycetes provides their active development (up to 8,150 thous CFU/g of dry compost), accumulation of carbon and nitrogen in compost, which improves the quality of the final product. Conclusion. The association of T. harzianum 128 micromycetes is characterized by high activity of enzymes of the cellulolytic complex, has a pronounced antagonistic activity against pathogens of certain diseases of crops. The introduction of T. harzianum 128 to composted chicken manure-based substrates reduces the composting time and improves the quality of the final product, which can increase the productivity of agrocenoses by including biofertilizers in crop fertilization systems.

scholarly journals Alternaria alternata and Neocosmospora sp. from the medicinal plant Euphorbia larica exhibit antagonistic activity against Fusarium sp., a plant pathogenic fungus

All Life ◽  
2020 ◽  
Vol 13 (1) ◽  
pp. 223-232 ◽  
Author(s):  
Fatma Khuseib Hamed Al-Rashdi ◽  
Abdullah Mohammed Al-Sadi ◽  
Bahja Z. Al-Riyamy ◽  
Sajeewa S. N. Maharachchikumbura ◽  
Huda Khalfan Al-Ruqaishi ◽  
...  
Keyword(s):  
Medicinal Plant ◽  
Alternaria Alternata ◽  
Pathogenic Fungus ◽  
Antagonistic Activity ◽  
Plant Pathogenic Fungus ◽  
Fusarium Sp

scholarly journals Eksplorasi Jamur Alkalotoleran dari Desa Sukolilo Barat, Kecamatan Labang, Kabupaten Bangkalan, Madura, Jawa Timur

2014 ◽  
Vol 16 (1) ◽  
pp. 10
Author(s):  
Helga Lusiana ◽  
MG Isworo Rukmi ◽  
Budi Raharjo
Keyword(s):  
Proteolytic Activity ◽  
Aspergillus Flavus ◽  
Trichoderma Harzianum ◽  
Dilution Method ◽  
Cellulolytic Activity ◽  
Trichoderma Longibrachiatum ◽  
Fusarium Sp

Mold exploration from limestone hills West Sukolilo  village, District Labang, Bangkalan, Madura, East Java,  conducted to found the  alkalotolerant mold isolates and investigate their  amylolytic, proteolytic, and cellulolytic activity. Mold isolation were done using spread plate and dilution method  on  PDA and CMA at pH 8.0, 9.0, and 10.0. Twenty-seven mold isolates have been found consist of  four genus  i.e. Aspergillus, Fusarium, Curvularia, and Trichoderma. The result of amylolytic test  showed Trichoderma longibrachiatum (IMD-26) have the  highest activity at pH 8, while  Fusarium sp 2 (IMD-24) showed the highest activity at pH 9. Aspergillus flavus (IMD-7) showed highest proteolytic activity at pH 8, while Hyphomycete (IMD-27) showed the highest activity at pH 9. The highest cellulolytic activity at pH 8 showed by Trichoderma harzianum (IMD-25) and pH 9 by Fusarium sporotrichoides (IMD-20). Keyword : alkalotoleran mold, amylolytic, proteolytic, cellulolytic.

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