Recurrent parent genome recovery analysis in a marker-assisted backcrossing program of rice (Oryza sativa L.)

2015 ◽  
Vol 338 (2) ◽  
pp. 83-94 ◽  
Author(s):  
Gous Miah ◽  
Mohd Y. Rafii ◽  
Mohd R. Ismail ◽  
Adam B. Puteh ◽  
Harun A. Rahim ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Recurrent Parent ◽  
Recurrent Parent Genome ◽  
Marker Assisted Backcrossing

Genetic dissection of the resistance to Rice stripe virus present in the indica rice cultivar ‘IR24’

Genome ◽  
2011 ◽  
Vol 54 (8) ◽  
pp. 611-619 ◽  
Author(s):  
Baoxiang Wang ◽  
Ling Jiang ◽  
Yingxin Zhang ◽  
Wenwei Zhang ◽  
Qi Wang ◽  
...  
Keyword(s):  
Oryza Sativa ◽  
De Novo ◽  
Oryza Sativa L ◽  
Indica Rice ◽  
Brown Planthopper ◽  
Rice Stripe Virus ◽  
Recurrent Parent ◽  
Chromosome 11 ◽  
Advanced Backcross ◽  
Rice Stripe Disease

Rice stripe disease, caused by Rice stripe virus (RSV) and transmitted by the small brown planthopper (Laodelphax striatellus Fallen), is one of the most serious viral diseases of rice in temperate East Asian production regions. Prior quantitative trait loci (QTL) mapping has established that Oryza sativa L. subsp. indica ‘IR24’ carries positive alleles at the three loci qSTV3, qSTV7, and qSTV11-i. Here, we report an advanced backcross analysis based on three selected chromosome segment substitution lines (CSSLs), each predicted to carry one of these three QTL. Three sets of BC4F2:3 populations were bred from a cross between the critical CSSL and its recurrent parent Oryza sativa L. subsp. japonica ‘Asominori’. Both qSTV3 and qSTV11-i were detected in their respective population, but qSTV7 was not. An allelic analysis based on a known carrier of the major RSV resistance gene Stvb-i, which is located on chromosome 11, showed that qSTV11-i was not allelic with Stvb-i. A large mapping population was used to delimit the location of qSTV11-i to a 73.6-kb region. The de novo markers developed for this purpose will be useful as marker-assisted selection tools in efforts to introduce qSTV11-i into breeding programmes aiming to improve the level of RSV resistance.

scholarly journals Rapid and targeted introgression of fgr gene through marker-assisted backcrossing in rice (Oryza sativa L.)

Genome ◽  
2017 ◽  
Vol 60 (12) ◽  
pp. 1045-1050 ◽  
Author(s):  
Acga Cheng ◽  
Ismanizan Ismail ◽  
Mohamad Osman ◽  
Habibuddin Hashim ◽  
Nur Samahah Mohd Zainual
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Cost Effective ◽  
Yield Potential ◽  
Recurrent Parent ◽  
Rice Varieties ◽  
Fragrant Rice ◽  
Self Sufficiency ◽  
Effective Manner ◽  
Fgr Gene

While it is crucial for developing countries like Malaysia to achieve self-sufficiency in rice (Oryza sativa L.), it is equally critical to be able to produce high-quality rice, specifically fragrant rice, which demands are often met through importation. The present study was aimed at developing high-yielding fragrant rice, in a timely and cost-effective manner. A marker-assisted backcross (MABC) approach was optimised to introgress the fragrance gene (fgr) into two high-yielding Malaysian varieties, MR84 and MR219, within two years utilising less than 50 molecular markers. Coupled with phenotypic screening, one single foreground marker (fgr-SNP) and 48 background markers were selected and utilised, revealing recovery of at least 90% of recurrent parent genome (RPG) in merely two backcross generations. Collectively, the yield potential of the developed BC2F2 lines (BLs) was higher (P > 0.05) than the donor parent, MRQ74, and similar (P < 0.05) to both the recurrent parents, MR84 and MR219. In addition, some of the developed BLs showed good grain quality, such as having long grain. We believe that this is the first report comprising the validation and utilisation of the single functional marker system (fgr-SNP) in introgressing the fgr gene into different rice varieties.

Keragaan Morfologi dan Kandungan Antosianin Padi Beras Merah (Oryza sativa L.) di Kecamatan Munte dan Kecamatan Payung, Kabupaten Karo

1970 ◽  
Vol 5 (3) ◽  
pp. 391-403
Author(s):  
Wiwik Winarti ◽  
Eva Sartini Bayu ◽  
Revandy Iskandar Damanik
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
International Rice Research Institute ◽  
Research Institute

Keragaan morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.) pada Kecamatan Munte dan Kecamatan Payung di Kabupaten Karo. Penelitian ini bertujuan untuk mendapatkan informasi morfologi dan kandungan antosianin padi beras merah (Oryza sativa L.). Penelitian ini dimulai dari Juni 2017dan selesai pada Januari 2018 di kecamatan Munte dan Kecamatan Payung Kabupaten Karo.Metode survei deskriptif menggunakan panduan International Rice Research Institute (IRRI). Teknik penentuan lokasi secara sengaja dan pengamblan sampel secara kebetulan.Uji kandungan antosianin menggunakan metode analitik dengan menghomogenkan sampel. Hasil eksplorasi didapatkan 72 genotipe yang dibagi menjadi tiga lokasi lahan. Berdasaran uji kandungan antosianin didapatkan lahan A memiliki kandungan tertinggi yaitu 0,5 mg/100 g dan terendah yaitu lahan B 0,08 mg/100 g.

Efficacy of some insecticide modules against major insect pests and spider population of rice, Oryza sativa L.

ENTOMON ◽  
2018 ◽  
Vol 43 (4) ◽  
pp. 257-262
Author(s):  
Atanu Seni ◽  
Bhimasen Naik
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Untreated Control ◽  
Insect Pests ◽  
Oryza Sativa L ◽  
Gall Midge ◽  
Stem Borer ◽  
The Other ◽  
The Third ◽  
Treated Plot

Experiments were carried out to assess some insecticide modules against major insect pests of rice. Each module consists of a basal application of carbofuran 3G @ 1 kg a.i ha-1 at 20 DAT and Rynaxypyr 20 SC @ 30 g a.i ha-1 at 45 DAT except untreated control. All modules differ with each other only in third treatment which was applied in 65 DAT. The third treatment includes: Imidacloprid 17.8 SL @ 27 g a.i ha-1, Pymetrozine 50 WG @ 150 g a.i ha-1, Triflumezopyrim 106 SC @ 27 g a.i ha-1, Buprofezin 25 SC @ 250 g a.i ha-1; Glamore (Imidacloprid 40+Ethiprole 40% w/w) 80 WG @ 100 g a.i. ha-1, Thiacloprid 24 SC @ 60 g a.i ha-1, Azadirachtin 0.03 EC @ 8 g a.i ha-1, Dinotefuran 20 SG@ 40 g a.i ha-1 and untreated control. All the treated plots recorded significantly lower percent of dead heart, white ear- head caused by stem borer and silver shoot caused by gall midge. Module with Pymetrozine 50 WG @ 150 g a.i ha-1 treated plot recorded significantly higher per cent reduction of plant hoppers (>80% over untreated control) and produced higher grain yield (50.75 qha-1) than the other modules. Among the different treated modules the maximum number of spiders was found in Azadirachtin 0.03 EC @ 8 g a.i ha-1 treated module plot followed by other treatments.

Correlation Studies on Grain Yield and its Component Traits in Rice (Oryza sativa L.)

2012 ◽  
Vol 2 (11) ◽  
pp. 13-14
Author(s):  
R. ARULMOZHI R. ARULMOZHI ◽  
◽  
Dr. A. MUTHUSWAMY Dr. A. MUTHUSWAMY
Keyword(s):  
Oryza Sativa ◽  
Grain Yield ◽  
Oryza Sativa L ◽  
Correlation Studies ◽  
Component Traits

Aplikasi Abu Sekam pada Padi Gogo (Oryza sativa L.) terhadap Kandungan Silikat dan Prolin Daun serta Amilosa dan Protein Biji

2011 ◽  
Vol 16 (1) ◽  
Author(s):  
Ahadiyat Yugi Rahayu ◽  
Tri Harjoso
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Screen House

Tujuan dari penelitian ini adalah untuk mengetahui pengaruh aplikasi abu sekam terhadap kandungan prolin dan silikat daun serta kualitas hasil yaitu protein dan amilosa biji padi gogo dan hubungan korelasi antar keempat komponen tersebut pada kondisi pertanaman 80 persen kapasitas lapang pada skala pot. Penelitian dilakukan di polibag dalam screen house Fakultas Pertanian Unsoed dengan menggunakan rancangan acak kelompok pola faktorial dengan faktor varietas (Situ patenggang, Limboto, Towuti, Batutegi dan Aek sibundong) dan faktor abu sekam (0, 2, 4, 6 t/ha), diulang tiga kali. Hasil penelitian menunjukan bahwa pemberian abu sekam dosis 2−6 t/ha mampu meningkatkan kandungan silikat daun antara 18,49−29,43% dan menurunkan kandungan amilosa biji pada lima varietas sekitar 4,19−6,92%. Pemberian abu sekam dosis 2−6 t/ha mampu meningkatkan kandungan prolin daun antara 27,56−70,63% dan protein biji antara 2,35−16,71%. Antarvarietas menunjukan bahwa kandungan prolin tertinggi dihasilkan oleh varietas Batu tegi 18,58 persen dan protein biji pada varietas Situ patenggang 9,55%. Terdapat korelasi antar karakter fisiologis yaitu antara silikat-prolin (0,62) dan kandungan protein-amilosa biji (-0,78).

Produksi dan Mutu Benih Padi (Oryza sativa L.) Ratun dengan Aplikasi Bakteri Synechococcus sp. pada Berbagai Salinitas Media

2017 ◽  
Author(s):  
Agung Sugiharto ◽  
Dwi Rahmawati ◽  
FNU Prayitno
Keyword(s):  
Oryza Sativa ◽  
Oryza Sativa L ◽  
Synechococcus Sp ◽  
Range Test

Salah satu upaya untuk meningkatkan produksi dan mutu benih di lahan salin yaitu melalui penggunaan metode ratun dengan penambahan bakteri sintetik Synechoccocus sp. Penelitian ini bertujuan untuk mengetahui produksi dan mutu benih padi ratun yang tercekam salinitas dengan penambahan bakteri sintetik Synechoccocus sp. Penelitian dilaksanakan pada bulan Juli - Desember 2015 di Desa Suco, Kecamatan Mumbul Sari Jember dan Laboratorium Teknologi Benih Politeknik Negeri Jember. Rancangan percobaan yang digunakan adalah Rancangan Acak Kelompok Faktorial (RAK) dengan 2 faktor dan 3 ulangan. Faktor pertama adalah cekaman Salinitas (S) yang terdiri dari 5 taraf, S1 = tanpa cekaman (kontrol), S2 = cekaman salinitas 1000 ppm, S3 = cekaman salinitas 2000 ppm, S4 = cekaman salinitas 3000 ppm, S5 = cekaman salinitas 4000 ppm. Faktor kedua inokulasi bakteri sintetik Synechoccocus sp. (B), yang terdiri dari 2 taraf, B1 = tanpa inokulasi bakteri (kontrol), B2 = Inokulasi sintetik Synechoccocus sp. Data dianalisis menggunakan uji F (ANOVA) dan dilanjutkan dengan perhitungan Duncan’s Multiple Range Test (DMRT). Hasil penelitian menunjukan perlakuan salinitas 1000 ppm (S2) menghasilkan jumlah anakan ratun produktif tertinggi yaitu 19,33 anakan. Cl- mempunyai fungsi utama dalam reaksi fotosintesis sehingga cekaman salintas pada perlakuan 1000 ppm (S2) dapat ditoleran oleh tanaman padi varietas Ciherang pada fase vegetatif. Pemberian Bakteri (B) Synechococcus sp. mampu menghasilkan tunas ratun tertinggi pada fase vegetatif yaitu 40,10 cm. Interaksi dari dua perlakuan menunjukan hasil yang nyata pada parameter jumlah gabah bernas yaitu cekaman salinitas 4000 ppm dengan inokulasi bakteri (B2S5) menghasilkan gabah bernas yang paling tinggi sebesar 99,06 butir. Inokulasi tanaman dengan Synechococcus sp. mampu meningkatkan kandungan nitrogen dan kandungan klorofil dalam jaringan tanaman. Interaksi antara cekaman salinitas 1000 ppm dengan inokulasi bakteri (B2S2) menghasilkan produksi per Ha yang paling tinggi yaitu 1,389 ton dan potensi produksi per Ha tertinggi yaitu 1.66 ton/ha.

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