Changes of pollen viability after preservation of Paeonia lactiflora in different provenances

Cryobiology ◽  
2022 ◽  
Author(s):  
Ruifen Ren ◽  
Jianzhou Gao ◽  
Hao Zhou ◽  
Mengting Zhu ◽  
Yan Liu
Keyword(s):  
Pollen Viability ◽  
Paeonia Lactiflora

scholarly journals The Cross Talk Between ROS And NO In The Viability Decline of Cryopreserved Pollen From Paeonia Lactiflora

2021 ◽  
Author(s):  
Ruifen Ren ◽  
Lingling Zhang ◽  
Hao Zhou ◽  
Xueru Jiang ◽  
Yan Liu
Keyword(s):  
Nadph Oxidase ◽  
Pollen Viability ◽  
Regulatory Gene ◽  
Enzyme Regulation ◽  
Nicotinamide Adenine Dinucleotide Phosphate ◽  
Paeonia Lactiflora ◽  
Positive Interaction ◽  
Cycle System ◽  
Cycle Systems ◽  
Key Sites

Abstract Reactive oxygen species (ROS) and nitric oxide (NO), two common active molecules, are both involved in changes in viability after liquid nitrogen (LN) storage, but the relationship between these two molecules has not been examined in plant cryopreservation until now. In this study, the pollen of Paeonia lactiflora 'Fen Yu Nu' with significantly decreased viability after cryopreservation was used as the material. We studied the effects of the two regulators on each other and their biosynthesis and scavenging indices to explore the interaction between ROS and NO in pollen cryopreservation and its mechanism. The results showed that the contents of ROS and NO increased significantly with the decrease of pollen viability after cryopreservation, and changes in the ROS and NO content had a significant effect on post-LN pollen viability. The ROS content positively regulated the endogenous NO content and had significant effects on the expression level of NOS-like enzyme regulation gene CSU2 and its activity. Down-regulated NO had a positive effect on the ROS content, and it significantly affected the expression of nicotinamide adenine dinucleotide phosphate (NADPH) oxidase and its regulatory gene RBOHJ. It also significantly affected catalase (CAT) and substrates related to the ascorbic acid (AsA)-glutathione (GSH) antioxidant cycle system. These results indicate that there was a positive interaction between ROS and NO in pollen cryopreservation. The NOS biosynthesis pathway is one of the ROS-regulated NO pathways, and the NADPH oxidase, CAT and AsA-GSH antioxidant cycle systems are the key sites of regulation of the ROS content by NO.

scholarly journals The Cross Talk Between ROS and NO in The Viability Declination of Cryopreserved-Pollen From Paeonia Lactiflora

2021 ◽  
Author(s):  
Ruifen Ren ◽  
Lingling Zhang ◽  
Hao Zhou ◽  
Xueru Jiang ◽  
Yan Liu
Keyword(s):  
Nadph Oxidase ◽  
Pollen Viability ◽  
Regulatory Gene ◽  
Enzyme Regulation ◽  
Paeonia Lactiflora ◽  
Positive Interaction ◽  
Cycle System ◽  
Key Sites ◽  
The Relationship ◽  
Positive Effect

Abstract Reactive oxygen species (ROS) and nitric oxide (NO), as two common active molecules, are both involved in the changes of viability after liquid nitrogen (LN) stored, but the relationship between these two molecules has not been involved in plant cryopreservation up to now. In this study, the pollen of Paeonia lactiflora 'Fen Yu Nu' with significantly decreased viability after cryopreservation as materials, by studied the effects of the two regulators on each other and their biosynthesis and scavenging indexes, to explore the interaction between ROS and NO in pollen cryopreservation and its mechanism.The results showed that: the contents of ROS and NO increased significantly with the decrease of pollen viability after cryopreservation, and regulated ROS and NO content had a significant effect on post-LN pollen viability; the changes of ROS content positively regulated the endogenous NO content, and had significant effects on the expression level of NOS-like enzyme regulation gene CSU2 and its activity; while regulated NO had a positive effect on ROS content, and significantly affected the expression of NADPH oxidase and its regulatory gene RBOHJ, and also significantly affected catalase (CAT) and the substrate related to ascorbic acid (AsA)- glutathione (GSH) antioxidant cycle system. These results indicate that there was a positive interaction between ROS and NO in the pollen cryopreservation, NOS biosynthesis pathway was one of the ROS regulated NO pathways, NADPH oxidase, CAT and AsA-GSH antioxidant cycle system were the key sites for NO regulated ROS content.

scholarly journals Pollen Germination in Different Genotypes of Gladiolus

1970 ◽  
Vol 11 ◽  
pp. 47-50
Author(s):  
Tul Bahadur Poon ◽  
TM Rao ◽  
C Aswath ◽  
PE Rajasekharan ◽  
DP Kumar
Keyword(s):  
Boric Acid ◽  
Magnesium Sulphate ◽  
Pollen Germination ◽  
Pollen Viability ◽  
Calcium Nitrate ◽  
Potassium Nitrate ◽  
Hybrid Selection ◽  
Fresh Pollen ◽  
Sterile Pollen ◽  
Germinated Pollen

Fresh pollen of 16 promising genotypes of gladiolus was tested for their pollen viability. Modified cellophane method was employed to assess the pollen viability. Pollen germination media consisted of 15% sucrose supplemented with 300 ppm calcium nitrate, 200 ppm magnesium sulphate, 100 ppm potassium nitrate and 100 ppm boric acid. Highly significant variations were observed for percentage of pollen germination, non-germinated pollen and sterile pollen. The highest pollen germination (76.41%) was in genotype Hybrid selection 88-10-22, and did not differ significantly from Gladiolus callianthus (75.41%), Sapna (75.10 %), Hybrid selection 86-32-11, (73.28 %), Kum Kum (69.41%), Poonam (69.22 %), Hybrid selection 87-22-1 (67.87 %), Hybrid selection 87-1-1 (67.61 %), Psittacinus hybrid (64.64%) and Darshan (63.97%). The lowest non- germinated pollen (10.47%) was in genotype Gladiolus callianthus, and insignificantly followed by Hybrid selection 88-10-22 with 18.77 % and Hybrid selection 87-22-1 with 18.95 %. The lowest percentage of sterile pollen was noticed in genotype Sapna (2.82%) followed by Poonam with 4.00 % Hybrid selection 88-10-22 with 4.82% and Hybrid selection 82-11-27 with 5.22%. Key words: Gladiolus; germination; pollen DOI: 10.3126/njst.v11i0.4089Nepal Journal of Science and Technology 11 (2010) 47-50

Floral morphology and pollen viability of an endangered and endemic Bromeliaceae species from the Atlantic Forest

Grana ◽  
2021 ◽  
pp. 1-20
Author(s):  
Valéria Leobina dos Santos ◽  
Catarina Carvalho Nievola ◽  
Adriana de Oliveira Fidalgo ◽  
Shoey Kanashiro ◽  
Maria das Graças Lapa Wanderley ◽  
...  
Keyword(s):  
Atlantic Forest ◽  
Floral Morphology ◽  
Pollen Viability

scholarly journals A Duplicated Copy of the Meiotic Gene ZIP4 Preserves up to 50% Pollen Viability and Grain Number in Polyploid Wheat

Biology ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 290
Author(s):  
Abdul Kader Alabdullah ◽  
Graham Moore ◽  
Azahara C. Martín
Keyword(s):  
Pollen Viability ◽  
Pollen Grains ◽  
Wheat Chromosome ◽  
Grain Number ◽  
Meiotic Gene ◽  
Chromosome 5B ◽  
Mutant Pollen ◽  
Female Gametogenesis ◽  
Chromosome 3B

Although most flowering plants are polyploid, little is known of how the meiotic process evolves after polyploidisation to stabilise and preserve fertility. On wheat polyploidisation, the major meiotic gene ZIP4 on chromosome 3B duplicated onto 5B and diverged (TaZIP4-B2). TaZIP4-B2 was recently shown to promote homologous pairing, synapsis and crossover, and suppress homoeologous crossover. We therefore suspected that these meiotic stabilising effects could be important for preserving wheat fertility. A CRISPR Tazip4-B2 mutant was exploited to assess the contribution of the 5B duplicated ZIP4 copy in maintaining pollen viability and grain setting. Analysis demonstrated abnormalities in 56% of meiocytes in the Tazip4-B2 mutant, with micronuclei in 50% of tetrads, reduced size in 48% of pollen grains and a near 50% reduction in grain number. Further studies showed that most of the reduced grain number occurred when Tazip4-B2 mutant plants were pollinated with the less viable Tazip4-B2 mutant pollen rather than with wild type pollen, suggesting that the stabilising effect of TaZIP4-B2 on meiosis has a greater consequence in subsequent male, rather than female gametogenesis. These studies reveal the extraordinary value of the wheat chromosome 5B TaZIP4-B2 duplication to agriculture and human nutrition. Future studies should further investigate the role of TaZIP4-B2 on female fertility and assess whether different TaZIP4-B2 alleles exhibit variable effects on meiotic stabilisation and/or resistance to temperature change.

scholarly journals Rising Atmospheric Temperature Impact on Wheat and Thermotolerance Strategies

Plants ◽  
2020 ◽  
Vol 10 (1) ◽  
pp. 43
Author(s):  
Adeel Khan ◽  
Munir Ahmad ◽  
Mukhtar Ahmed ◽  
M. Iftikhar Hussain
Keyword(s):  
High Temperature ◽  
Industrial Revolution ◽  
Foliar Application ◽  
Pollen Viability ◽  
Grain Filling ◽  
Crop Productivity ◽  
Potassium Nitrate ◽  
Agricultural Crop ◽  
Stay Green ◽  
Seed Formation

Temperature across the globe is increasing continuously at the rate of 0.15–0.17 °C per decade since the industrial revolution. It is influencing agricultural crop productivity. Therefore, thermotolerance strategies are needed to have sustainability in crop yield under higher temperature. However, improving thermotolerance in the crop is a challenging task for crop scientists. Therefore, this review work was conducted with the aim of providing information on the wheat response in three research areas, i.e., physiology, breeding, and advances in genetics, which could assist the researchers in improving thermotolerance. The optimum temperature for wheat growth at the heading, anthesis, and grain filling duration is 16 ± 2.3 °C, 23 ± 1.75 °C, and 26 ± 1.53 °C, respectively. The high temperature adversely influences the crop phenology, growth, and development. The pre-anthesis high temperature retards the pollen viability, seed formation, and embryo development. The post-anthesis high temperature declines the starch granules accumulation, stem reserve carbohydrates, and translocation of photosynthates into grains. A high temperature above 40 °C inhibits the photosynthesis by damaging the photosystem-II, electron transport chain, and photosystem-I. Our review work highlighted that genotypes which can maintain a higher accumulation of proline, glycine betaine, expression of heat shock proteins, stay green and antioxidant enzymes activity viz., catalase, peroxidase, super oxide dismutase, and glutathione reductase can tolerate high temperature efficiently through sustaining cellular physiology. Similarly, the pre-anthesis acclimation with heat treatment, inorganic fertilizer such as nitrogen, potassium nitrate and potassium chloride, mulches with rice husk, early sowing, presoaking of a 6.6 mM solution of thiourea, foliar application of 50 ppm dithiothreitol, 10 mg per kg of silicon at heading and zinc ameliorate the crop against the high temperature. Finally, it has been suggested that modern genomics and omics techniques should be used to develop thermotolerance in wheat.

PHYSIOLOGICAL RESPONSE AND POLLEN VIABILITY OF PISUM SATIVUM GENOTYPES UNDER HIGH TEMPERATURE INFLUENCE

2009 ◽  
pp. 665-672 ◽  
Author(s):  
V. Petkova ◽  
V. Nikolova ◽  
S.H. Kalapchieva ◽  
V. Stoeva ◽  
E. Topalova ◽  
...  
Keyword(s):  
High Temperature ◽  
Pisum Sativum ◽  
Physiological Response ◽  
Pollen Viability ◽  
Temperature Influence

scholarly journals Cosegregation of Single Genes Associated with Fertility Restoration and Transcript Processing of Sorghum Mitochondrial orf107 and urf209

Genetics ◽  
1998 ◽  
Vol 150 (1) ◽  
pp. 383-391 ◽  
Author(s):  
Hoang V Tang ◽  
Ruying Chang ◽  
Daryl R Pring
Keyword(s):  
Fertility Restoration ◽  
Mitochondrial Gene ◽  
Pollen Viability ◽  
Single Gene ◽  
Dominant Gene ◽  
Nuclear Gene ◽  
Male Sterile ◽  
Reading Frame ◽  
Transcript Processing ◽  
Backcross Populations

Abstract Defective nuclear-cytoplasmic interactions leading to aberrant microgametogenesis in sorghum carrying the IS1112C male-sterile cytoplasm occur very late in pollen maturation. Amelioration of this condition, the restoration of pollen viability, involves a novel two-gene gametophytic system, wherein genes designated Rf3 and Rf4 are required for viability of individual gametes. Rf3 is tightly linked to, or represents, a single gene that regulates a transcript processing activity that cleaves transcriptsof orf107, a chimeric mitochondrial open reading frame specific to IS1112C. The mitochondrial gene urf 209 is also subject to nucleus-specific enhanced transcript processing, 5′ to the gene, conferred by a single dominant gene designated Mmt1. Examinations of transcript patterns in F2 and two backcross populations indicated cosegregation of the augmented orf107 and urf209 processing activities in IS1112C. Several sorghum lines that do not restore fertility or confer orf107 transcript processing do exhibit urf209 transcript processing, indicating that the activities are distinguishable. We conclude that the nuclear gene(s) conferring enhanced orf107 and urf209 processing activities are tightly linked in IS1112C. Alternatively, the similarity in apparent regulatory action of the genes may indicate allelic differences wherein the IS1112C Rf3 allele may differ from alleles of maintainer lines by the capability to regulate both orf107 and urf209 processing activities.

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