scholarly journals Prepatterning of Developmental Gene Expression by Modified Histones before Zygotic Genome Activation

2011 ◽  
Vol 21 (6) ◽  
pp. 993-1004 ◽  
Author(s):  
Leif C. Lindeman ◽  
Ingrid S. Andersen ◽  
Andrew H. Reiner ◽  
Nan Li ◽  
Håvard Aanes ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zygotic Genome Activation ◽  
Developmental Gene ◽  
Developmental Gene Expression ◽  
Genome Activation ◽  
Zygotic Genome

scholarly journals Combinatorial action of NF–Y and TALE at embryonic enhancers defines distinct gene expression programs during zygotic genome activation in zebrafish

2020 ◽  
Vol 459 (2) ◽  
pp. 161-180 ◽  
Author(s):  
William Stanney ◽  
Franck Ladam ◽  
Ian J. Donaldson ◽  
Teagan J. Parsons ◽  
René Maehr ◽  
...  
Keyword(s):  
Gene Expression ◽  
Zygotic Genome Activation ◽  
Distinct Gene ◽  
Genome Activation ◽  
Zygotic Genome

scholarly journals TALE and NF-Y co-occupancy marks enhancers of developmental control genes during zygotic genome activation in zebrafish

2019 ◽  
Author(s):  
William Stanney ◽  
Franck Ladam ◽  
Ian J. Donaldson ◽  
Teagan J. Parsons ◽  
René Maehr ◽  
...  
Keyword(s):  
Gene Expression ◽  
Transcriptional Regulation ◽  
Transcription Factors ◽  
Maternal Factors ◽  
Zygotic Genome Activation ◽  
Enhancer Activity ◽  
Genome Activation ◽  
Expression Program ◽  
Zygotic Genome

SUMMARYAnimal embryogenesis is initiated by maternal factors, but zygotic genome activation (ZGA) shifts control to the embryo at early blastula stages. ZGA is thought to be mediated by specialized maternally deposited transcription factors (TFs), but here we demonstrate that NF-Y and TALE – TFs with known later roles in embryogenesis – co-occupy unique genomic elements at zebrafish ZGA. We show that these elements are selectively associated with early-expressed genes involved in transcriptional regulation and possess enhancer activity in vivo. In contrast, we find that elements individually occupied by either NF-Y or TALE are associated with genes acting later in development – such that NF-Y controls a cilia gene expression program while TALE TFs control expression of hox genes. We conclude that NF-Y and TALE have a shared role at ZGA, but separate roles later during development, demonstrating that combinations of known TFs can regulate subsets of key developmental genes at vertebrate ZGA.

scholarly journals The histone deacetylase activity of HDAC1/2 is required to safeguard zygotic genome activation in mice and cattle

2021 ◽  
Author(s):  
Yanna Dang ◽  
Shuang Li ◽  
Panpan Zhao ◽  
Lieying Xiao ◽  
Lefeng Wang ◽  
...  
Keyword(s):  
Gene Expression ◽  
Histone Deacetylase ◽  
Dynamic Change ◽  
Ectopic Expression ◽  
Gene Expression Pattern ◽  
Subsequent Development ◽  
Dominant Negative Mutant ◽  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome

ABSTRACTThe genome is transcriptionally inert at fertilization and must be activated through a remarkable developmental process called zygotic genome activation (ZGA). The gene expression pattern formed over the course of ZGA is required for establishing totipotency in early embryos and subsequent development. Substantial epigenetic reprogramming contributes significantly to the pronounced change in gene expression during ZGA, however the mechanism has yet to be resolved. Here, we find histone deacetylase 1 and 2 (HDAC1/2) are critical histone modifiers that regulate ZGA through the histone deacetylase activity. Notably, we show that H3K27ac level declines dramatically during ZGA with a dynamic change in its genome-wide distribution. In mouse embryos, ectopic expression of HDAC1/2 dominant negative mutant leads to a failure of H3K27ac removal and a developmental arrest at 2-cell stage. RNA-seq results reveal a remarkable transcriptomic change with 6565 differentially expressed genes identified. Further analysis shows 64% of down-regulated genes are ZGA genes and 49% of up-regulated genes are developmental genes. Low input ChIP-seq analysis exhibits an increase and decrease of H3K27ac enrichment at the promoter region of up- and down-regulated genes, respectively. Moreover, HDAC1 mutants prohibited removal of broad H3K4me3 domain via impeding the expression of Kdm5s during ZGA. Importantly, the developmental block can be greatly overcome through injection of Kdm5b mRNA and expression of the majority of dysregulated genes partially corrected. Similar functional significance of HDAC1/2 in ZGA is conserved in bovine embryos. Together, we propose that HDAC1/2 is indispensable for mouse and bovine ZGA via creating correct transcriptional repressive and active states.

scholarly journals Maternal Style Selectively Shapes Amygdalar Development and Social Behavior in Rats Genetically Prone to High Anxiety

2015 ◽  
Vol 37 (3) ◽  
pp. 203-214 ◽  
Author(s):  
Joshua L. Cohen ◽  
Matthew E. Glover ◽  
Phyllis C. Pugh ◽  
Andrew D. Fant ◽  
Rebecca K. Simmons ◽  
...  
Keyword(s):  
Gene Expression ◽  
Psychological Health ◽  
Early Life ◽  
Developmental Trajectory ◽  
Emotional Behavior ◽  
Developmental Gene ◽  
Developmental Gene Expression ◽  
Maternal Style ◽  
Anxiety Depression ◽  
Cross Fostering

The early-life environment critically influences neurodevelopment and later psychological health. To elucidate neural and environmental elements that shape emotional behavior, we developed a rat model of individual differences in temperament and environmental reactivity. We selectively bred rats for high versus low behavioral response to novelty and found that high-reactive (bred high-responder, bHR) rats displayed greater risk-taking, impulsivity and aggression relative to low-reactive (bred low-responder, bLR) rats, which showed high levels of anxiety/depression-like behavior and certain stress vulnerability. The bHR/bLR traits are heritable, but prior work revealed bHR/bLR maternal style differences, with bLR dams showing more maternal attention than bHRs. The present study implemented a cross-fostering paradigm to examine the contribution of maternal behavior to the brain development and emotional behavior of bLR offspring. bLR offspring were reared by biological bLR mothers or fostered to a bLR or bHR mother and then evaluated to determine the effects on the following: (1) developmental gene expression in the hippocampus and amygdala and (2) adult anxiety/depression-like behavior. Genome-wide expression profiling showed that cross-fostering bLR rats to bHR mothers shifted developmental gene expression in the amygdala (but not hippocampus), reduced adult anxiety and enhanced social interaction. Our findings illustrate how an early-life manipulation such as cross-fostering changes the brain's developmental trajectory and ultimately impacts adult behavior. Moreover, while earlier studies highlighted hippocampal differences contributing to the bHR/bLR phenotypes, our results point to a role of the amygdala as well. Future work will pursue genetic and cellular mechanisms within the amygdala that contribute to bHR/bLR behavior either at baseline or following environmental manipulations.

scholarly journals Identification of Early Zygotic Genes in the Yellow Fever Mosquito Aedes aegypti and Discovery of a Motif Involved in Early Zygotic Genome Activation

PLoS ONE ◽  
2012 ◽  
Vol 7 (3) ◽  
pp. e33933 ◽  
Author(s):  
James K. Biedler ◽  
Wanqi Hu ◽  
Hongseok Tae ◽  
Zhijian Tu
Keyword(s):  
Aedes Aegypti ◽  
Yellow Fever ◽  
Yellow Fever Mosquito ◽  
Zygotic Genome Activation ◽  
Genome Activation ◽  
Zygotic Genome

Developmental gene expression in cancer

1979 ◽  
Vol 560 (2) ◽  
pp. 243-280 ◽  
Author(s):  
K Ibsen
Keyword(s):  
Gene Expression ◽  
Developmental Gene ◽  
Developmental Gene Expression

scholarly journals Zygotic Genome Activation Triggers the DNA Replication Checkpoint at the Midblastula Transition

Cell ◽  
2015 ◽  
Vol 160 (6) ◽  
pp. 1169-1181 ◽  
Author(s):  
Shelby A. Blythe ◽  
Eric F. Wieschaus
Keyword(s):  
Dna Replication ◽  
Zygotic Genome Activation ◽  
Midblastula Transition ◽  
Replication Checkpoint ◽  
Dna Replication Checkpoint ◽  
Genome Activation ◽  
Zygotic Genome

CLAMP regulates Zygotic Genome Activation in Drosophila embryos

Genetics ◽  
2021 ◽  
Author(s):  
Megan M Colonnetta ◽  
Juan E Abrahante ◽  
Paul Schedl ◽  
Daryl M Gohl ◽  
Girish Deshpande
Keyword(s):  
Temporal Dynamics ◽  
Embryonic Patterning ◽  
Zygotic Genome Activation ◽  
Promoter Sequences ◽  
Genome Wide ◽  
Zygotic Transcription ◽  
Pioneer Factor ◽  
Genome Activation ◽  
Drosophila Embryos ◽  
Zygotic Genome

Abstract Embryonic patterning is critically dependent on zygotic genome activation (ZGA). In Drosophila melanogaster embryos, the pioneer factor Zelda directs ZGA, possibly in conjunction with other factors. Here we have explored novel involvement of Chromatin-Linked Adapter for MSL Proteins (CLAMP) during ZGA. CLAMP binds thousands of sites genome-wide throughout early embryogenesis. Interestingly, CLAMP relocates to target promoter sequences across the genome when ZGA is initiated. Although there is a considerable overlap between CLAMP and Zelda binding sites, the proteins display distinct temporal dynamics. To assess whether CLAMP occupancy affects gene expression, we analyzed transcriptomes of embryos zygotically compromised for either clamp or zelda and found that transcript levels of many zygotically-activated genes are similarly affected. Importantly, compromising either clamp or zelda disrupted the expression of critical segmentation and sex determination genes bound by CLAMP (and Zelda). Furthermore, clamp knockdown embryos recapitulate other phenotypes observed in Zelda-depleted embryos, including nuclear division defects, centrosome aberrations, and a disorganized actomyosin network. Based on these data, we propose that CLAMP acts in concert with Zelda to regulate early zygotic transcription.

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