Expression of thymosin beta-4 in human periodontal ligament cells and mouse periodontal tissue and its role in osteoblastic/cementoblastic differentiation

2015 ◽  
Vol 90 (1-3) ◽  
pp. 16-26 ◽  
Author(s):  
Sang-Im Lee ◽  
Deok-Won Lee ◽  
Hyung-Mun Yun ◽  
Hee-Jae Cha ◽  
Cheol-Hyeon Bae ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Thymosin Beta 4 ◽  
Human Periodontal Ligament Cells

scholarly journals Effects of methanandamide on human periodontal ligament cells

2019 ◽  
Author(s):  
Fengqiu Zhang ◽  
Burcu Özdemir ◽  
Phuong Quynh Nguyen ◽  
Oleh Andrukhov ◽  
Xiaohui Rausch-Fan
Keyword(s):  
Periodontal Ligament ◽  
Cannabinoid Receptors ◽  
Endocannabinoid System ◽  
Periodontal Ligament Cells ◽  
Periodontal Tissue ◽  
Inflammatory Conditions ◽  
Human Periodontal Ligament Cells ◽  
Chemotactic Protein ◽  
Improved Stability ◽  
Porphyromonas Gingivalis Lipopolysaccharide

Abstract Background Endocannabinoid system is involved in the regulation of periodontal tissue homeostasis. Synthetic cannabinoid methanandamide (Meth-AEA) has an improved stability and affinity to cannabinoid receptors compared to its endogenous analogue anandamide. In the present study, we investigated the effect of methanandamide on the production of pro-inflammatory mediators in primary human periodontal ligament cells (hPdLCs). Methods hPdLCs were treated with Meth-AEA for 24 h and resulting production of interleukin (IL)-6, IL-8, and monocyte chemotactic protein (MCP)-1 was measured under normal condition as well as under inflammatory conditions mimicked by the presence of Porphyromonas gingivalis lipopolysaccharide (LPS). Additionally, the effect of Meth-AEA on the proliferation/viability of hPdLCs was measured by MTT method. Results Methanandamide at concentration of 10 µM significantly inhibited P. gingivalis LPS induced production of IL-6, IL-8, and MCP-1. Basal production of IL-6 and IL-8 was slightly enhanced by 10 µM Meth-AEA. No effect of Meth-AEA on the basal production of MCP-1 was observed. Proliferation/viability of hPdLCs was not affected by Meth-AEA in concentrations up to 10 µM and was significantly inhibited by 30 µM Meth-AEA. Conclusion Our study supports the influence of cannabinoid system on the inflammatory processes in periodontal tissue and its potential involvement in the progression of periodontal disease.

scholarly journals Thymosin Beta-4 Suppresses Osteoclastic Differentiation and Inflammatory Responses in Human Periodontal Ligament Cells

PLoS ONE ◽  
2016 ◽  
Vol 11 (1) ◽  
pp. e0146708 ◽  
Author(s):  
Sang-Im Lee ◽  
Jin-Kyu Yi ◽  
Won-Jung Bae ◽  
Soojung Lee ◽  
Hee-Jae Cha ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
Inflammatory Responses ◽  
Periodontal Ligament Cells ◽  
Thymosin Beta 4 ◽  
Human Periodontal Ligament Cells ◽  
Osteoclastic Differentiation

GDNF From Human Periodontal Ligament Cells Treated With Pro-Inflammatory Cytokines Promotes Neurocytic Differentiation of PC12 Cells

2016 ◽  
Vol 118 (4) ◽  
pp. 699-708
Author(s):  
Shinichiro Yoshida ◽  
Naohide Yamamoto ◽  
Naohisa Wada ◽  
Atsushi Tomokiyo ◽  
Daigaku Hasegawa ◽  
...  
Keyword(s):  
Pc12 Cells ◽  
Inflammatory Cytokines ◽  
Periodontal Ligament ◽  
Periodontal Ligament Cells ◽  
Human Periodontal Ligament Cells ◽  
Pro Inflammatory Cytokines

Potential of the propolis as storage medium to preserve the viability of cultured human periodontal ligament cells: an in vitro study

2011 ◽  
Vol 27 (2) ◽  
pp. 102-108 ◽  
Author(s):  
Payal Saxena ◽  
Vandana Aditya Pant ◽  
Kulvindar Kaur Wadhwani ◽  
Mahendra Pratap Kashyap ◽  
Saurabh Kumar Gupta ◽  
...  
Keyword(s):  
Periodontal Ligament ◽  
In Vitro Study ◽  
Vitro Study ◽  
Periodontal Ligament Cells ◽  
Storage Medium ◽  
Human Periodontal Ligament Cells
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