scholarly journals Interaction between dolutegravir and folate transporters and receptor in human and rodent placenta

EBioMedicine ◽  
2022 ◽  
Vol 75 ◽  
pp. 103771
Author(s):  
Julian C. Gilmore ◽  
Md. Tozammel Hoque ◽  
Wanying Dai ◽  
Haneesha Mohan ◽  
Caroline Dunk ◽  
...  
Keyword(s):  
Folate Transporters

OR015: ROUX-EN-Y Gastric Bypass Surgery and Folate Deficiency: Contributions of Reduced Gene Expression of Folate Transporters (SLC19A1 AND SLC46A1) in Addition to Low Folate Intake

2015 ◽  
Vol 34 ◽  
pp. S5-S6
Author(s):  
P.C. Sala ◽  
G. Belarmino ◽  
N.M. Machado ◽  
M.M. Silva ◽  
R. Dippolito ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gastric Bypass ◽  
Bypass Surgery ◽  
Gastric Bypass Surgery ◽  
Folate Deficiency ◽  
Folate Intake ◽  
Folate Transporters

Expression of folate transporters in human placenta and implications for homocysteine metabolism

Placenta ◽  
2010 ◽  
Vol 31 (2) ◽  
pp. 134-143 ◽  
Author(s):  
N. Solanky ◽  
A. Requena Jimenez ◽  
S.W. D'Souza ◽  
C.P. Sibley ◽  
J.D. Glazier
Keyword(s):  
Human Placenta ◽  
Folate Transporters

scholarly journals STRUCTURAL COMPARISON OF PROKARYOTIC AND EUKARYOTIC FOLATE TRANSPORTERS BY COMPUTATIONAL APPROACH

2018 ◽  
Vol 11 (14) ◽  
pp. 19
Author(s):  
Deepa Agarwal ◽  
Alok Jha
Keyword(s):  
Homo Sapiens ◽  
New Drugs ◽  
Transmembrane Helices ◽  
Ramachandran Plot ◽  
Functional Prediction ◽  
Structural Comparison ◽  
Folate Receptors ◽  
Realistic Representation ◽  
Folate Transporters ◽  
Membrane Spanning

Objective: In silico approach has particularly drawn attention in providing a realistic representation needed to understand the fundamental molecular structure of a transporter. The importance of folate metabolism and role in the internalization of antifolates in eukaryotes have been studied extensively, but the structural study of folate transporters in Homo sapiens (HFT), Plasmodium falciparum (PFT), and Streptococcus sp. (SFT) is still lacking. This study was conducted to study and compare the structures of prokaryotic and eukaryotic folate transporters. Methods: HFT, PFT, and SFT were queried using blast and sequences were retrieved using National Center for Biotechnology and Information (NCBI) databases. This was superseded by structural and functional prediction of transporters. The structure has been generated using Swiss model which was visualized using PyMol and validated by Procheck and ERRAT analysis along with the values of different secondary structures mapping to diverse sections of the Ramachandran plot. The structural and functional comparison was performed by PROSO, ProFunc, TM Score, Porewalker, TMHMM, and Protscale. Result: All the parameters for structural comparison suggest that H. sapiens folate transporter is 16.67% and 17.72% identical to Plasmodium and Streptococcus whereas Plasmodium is 21.59% identical to Streptococcus. The evaluation of transmembrane helices and hydrophobicity resulted in the presence of 1, 4, and 12 membrane-spanning segments with predicted US, UDUD, and UDS as pore shape in Plasmodium, Streptococcus, and humans. Conclusion: Such folate receptors are the main targets for the specific conveyance of antifolates. The differences found between these species may offer possibilities for the development of new drugs in future. 

scholarly journals 401: The differential expression of folate transporters in the fetal liver during early gestation

2011 ◽  
Vol 204 (1) ◽  
pp. S162-S163
Author(s):  
Devika Maulik ◽  
Bradley Thomas ◽  
Roger Gaedigk ◽  
J. Steven Leeder
Keyword(s):  
Differential Expression ◽  
Fetal Liver ◽  
Early Gestation ◽  
Folate Transporters

scholarly journals Regulatory mechanisms of intestinal folate uptake in a rat model of folate oversupplementation

2010 ◽  
Vol 105 (6) ◽  
pp. 827-835 ◽  
Author(s):  
Som Dev ◽  
Nissar Ahmad Wani ◽  
Jyotdeep Kaur
Keyword(s):  
Folic Acid ◽  
Membrane Vesicles ◽  
Mrna Levels ◽  
Uptake System ◽  
Folate Transport ◽  
Down Regulation ◽  
Ph Optimum ◽  
Nucleotide Biosynthesis ◽  
Simple Diffusion ◽  
Folate Transporters

Folic acid is essential for numerous biological functions, ranging from nucleotide biosynthesis to the remethylation of homocysteine. Folic acid is unable to cross the biological membranes by simple diffusion, so there exists a well-developed epithelial folate transport system for the regulation of normal folate homeostasis in the intestine. Any perturbances in the folate uptake system might lead to a state of folate deficiency, which in turn is strongly associated with the risk of various cancers, birth defects and CVD. Countries with obligatory folate fortification of food (USA and Canada) have documented a significant decrease in neural tube defects in newborns. However, the effect of folate oversupplementation on the intestinal absorption of folic acid has not been studied. We studied the process of folate transport and the expression of folate transporters in the rat intestine after folate oversupplementation. Rats were oversupplemented with tenfold the normal requirement of folic acid for periods of 10 and 60 d. Folate uptake in intestinal brush-border membrane vesicles followed saturable kinetics with pH optimum at 5·5. Acute, but not chronic, folate oversupplementation led to a significant down-regulation in intestinal folate uptake at acidic pH optima and was associated with a decrease in Vmax without any significant change in the Km of the folate uptake process. The decrease in folate uptake was also associated with the down-regulation in the protein levels of major folate transporters, proton-coupled folate transporter (PCFT) and reduced folate carrier (RFC), without altering their mRNA levels. Hence, it was concluded that acute folate oversupplementation results in a significant decrease in intestinal folate uptake by down-regulating the expressions of RFC and PCFT, via some post-transcriptional or translational mechanisms.

Increased synthesis of folate transporters regulates folate transport in conditions of ethanol exposure and folate deficiency

2015 ◽  
Vol 411 (1-2) ◽  
pp. 151-160 ◽  
Author(s):  
Shilpa Thakur ◽  
Deepti More ◽  
Beenish Rahat ◽  
Krishan Lal Khanduja ◽  
Jyotdeep Kaur
Keyword(s):  
Folate Deficiency ◽  
Ethanol Exposure ◽  
Folate Transport ◽  
Folate Transporters

scholarly journals Folate Supplementation during Oocyte Maturation Positively Impacts the Folate-Methionine Metabolism in Pre-Implantation Embryos

2021 ◽  
Author(s):  
Sikander Saini ◽  
Vishal Sharma ◽  
Shama Ansari ◽  
Amit Kumar ◽  
Abhishek Thakur ◽  
...  
Keyword(s):  
Folic Acid ◽  
Production Rate ◽  
Oocyte Maturation ◽  
Folic Acid Supplementation ◽  
Folate Metabolism ◽  
Acid Supplementation ◽  
Maturation Medium ◽  
Metabolism Enzymes ◽  
Methionine Cycle ◽  
Folate Transporters

Abstract Folic acid is vital for DNA synthesis and methylations through one-carbon (C1) metabolism. Thus, it is essential for cell division during embryonic development. The present study investigated the effect of folic acid supplementation on oocyte maturation, blastocyst development and the expression of folate transporters as well as folate metabolism enzymes in oocytes and pre-implantation embryos of goat. Immature goat oocytes, matured in maturation medium comprising different folic acid concentrations (0, 10, 50, 100 and 150 µM), were in vitro fertilized and cultured. Cumulus expansion markers (Ptx3 and Ptgs2) in cumulus cells were highly upregulated after 50 µM folic acid supplementation indicating higher degree of maturation. Supplementation of 50 µM folic acid during oocyte maturation resulted in significantly higher blastocyst production rate, reduction in intracellular ROS levels as well as upregulation of the transcripts for folate transporters and key folate-methionine cycle enzymes in comparison to control. The present study demonstrates the existence of active folate-methionine cycle in oocytes and pre-implantation goat embryos. Supplementation of 50 µM folic acid in maturation medium increases the blastocyst production rate, improves oocyte maturation, reduces ROS production as well as upregulate the expression of Folr1 and folate metabolism enzyme, Mtr.

84 Folate-methionine cycle and folate transport in developing buffalo embryos

2020 ◽  
Vol 32 (2) ◽  
pp. 168
Author(s):  
S. Ansari ◽  
S. Jamwal ◽  
S. Saini ◽  
R. Singh ◽  
D. Malakar
Keyword(s):  
Folic Acid ◽  
Carbon Metabolism ◽  
Ovarian Tissue ◽  
Cumulus Cells ◽  
Folate Transport ◽  
Nucleotide Synthesis ◽  
Folate Concentration ◽  
Vitamin B9 ◽  
Methionine Cycle ◽  
Folate Transporters

Periconceptional folic acid is known to have a major role in the prevention of neural tube defects, leading to global recommendations for folic acid supplementation before and in early pregnancy. Maternal folate throughout pregnancy may have other roles in offspring health, including neurodevelopment and cognitive performance in childhood. Folate and folic acid (vitamin B9) act as a co-enzyme essential for single carbon metabolism, a network of pathways involved in several biological processes including nucleotide synthesis, DNA repair, and methylation reactions. In general, rapidly growing and multiplying cells require an adequate supply of folate. A primary deficiency of natural folate resulting in an increase of the total homocysteine concentration may be detrimental to the quality of the oocyte, subsequent fertilisation, embryogenesis, implantation, and fetal development. However, to date, folate-methionine metabolism and folate transport have not been studied in developing buffalo embryos. The present study details transcript expression for genes encoding key enzymes in the linked folate-methionine cycles in the ovary tissue, cumulus cells, immature oocytes, IVM oocytes, and pre-implantation embryos and also estimates the folate concentration in follicular fluid (FF) of buffalo. The FF was pooled and collected by aspiration of different sizes of surface follicles (2-8mm diameter). The total number of analysed samples was three, with different dilutions and estimation of folate in FF of buffalo done by chemiluminescence assay. Total RNA was extracted from oocytes, cumulus cells, ovarian tissue, and embryos produced from IVF. RT-PCR was performed to analyse the expression of folate-methionine cycle enzymes and folate transporters. Transcripts for all the enzymes of the folate-methionine cycle (i.e. SHMT, MTR, MTRR, MAT1A, MAT2B, GNMT, AHCY, CBS, and DHFR) and folate transporters (FOLR1, FOLR2) and reduced folate carrier (SLC19A1) were expressed in ovarian tissue, cumulus cells, oocytes, and pre-implantation embryos. Immunocytochemical analysis revealed FOLR2 and SLC19A1 protein expression on the plasma membrane and/or cytoplasm of the oocytes and embryos, and FOLR1 in the nucleus of pre-implantation embryos. The folate concentration in FF was 24ngmL−1. This is the first report to examine the concentration of folate in FF and revealed the identification of transcripts in different samples of buffalo species. The presence of these enzymes could have a profound effect on single-carbon metabolism within the ovary and pre-implantation embryo, therefore indicating that folate from FF is being disseminated through folate receptors within oocytes and embryos to participate in the folate pathway. This study advocates the necessity for examination of the result of folate supplementation throughout invitro embryo production for improving the quality and quantity of transferable blastocysts and subsequently live calf births in buffalo.

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