Peptidomic analysis of zebrafish embryos exposed to polychlorinated biphenyls and their impact on eye development

2019 ◽  
Vol 175 ◽  
pp. 164-172 ◽  
Author(s):  
Yue Jiang ◽  
Shuchun Zhang ◽  
Xin Zhang ◽  
Nan Li ◽  
Qingyu Zhang ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls ◽  
Eye Development ◽  
Zebrafish Embryos ◽  
Peptidomic Analysis

miR-21 is involved in skeletal deficiencies of zebrafish embryos exposed to polychlorinated biphenyls

2016 ◽  
Vol 24 (1) ◽  
pp. 886-891 ◽  
Author(s):  
Li Ju ◽  
Zhiwen Zhou ◽  
Bo Jiang ◽  
Yue Lou ◽  
Zhiqun Zhang
Keyword(s):  
Polychlorinated Biphenyls ◽  
Zebrafish Embryos

scholarly journals Early retinoic acid deprivation in developing zebrafish results in microphthalmia

2012 ◽  
Vol 29 (4-5) ◽  
pp. 219-228 ◽  
Author(s):  
HONG-GAM T. LE ◽  
JOHN E. DOWLING ◽  
D. JOSHUA CAMERON
Keyword(s):  
Retinoic Acid ◽  
Vitamin A ◽  
Eye Development ◽  
Vitamin A Deficiency ◽  
Vital Role ◽  
Zebrafish Embryos ◽  
Developmental Abnormalities ◽  
Optokinetic Reflex ◽  
Eye Size ◽  
And Behavior

AbstractVitamin A deficiency causes impaired vision and blindness in millions of children around the world. Previous studies in zebrafish have demonstrated that retinoic acid (RA), the acid form of vitamin A, plays a vital role in early eye development. The objective of this study was to describe the effects of early RA deficiency by treating zebrafish with diethylaminobenzaldehyde (DEAB), a potent inhibitor of the enzyme retinaldehyde dehydrogenase (RALDH) that converts retinal to RA. Zebrafish embryos were treated for 2 h beginning at 9 h postfertilization. Gross morphology and retinal development were examined at regular intervals for 5 days after treatment. The optokinetic reflex (OKR) test, visual background adaptation (VBA) test, and the electroretinogram (ERG) were performed to assess visual function and behavior. Early treatment of zebrafish embryos with 100 μM DEAB (9 h) resulted in reduced eye size, and this microphthalmia persisted through larval development. Retinal histology revealed that DEAB eyes had significant developmental abnormalities but had relatively normal retinal lamination by 5.5 days postfertilization. However, the fish showed neither an OKR nor a VBA response. Further, the retina did not respond to light as measured by the ERG. We conclude that early deficiency of RA during eye development causes microphthalmia as well as other visual defects, and that timing of the RA deficiency is critical to the developmental outcome.

Anisodamine affects the pigmentation, mineral density, craniofacial area, and eye development in zebrafish embryos

2021 ◽  
Author(s):  
Binjie Wang ◽  
Tianyi Chen ◽  
Anli Wang ◽  
Jiakai Fang ◽  
Jiye Wang ◽  
...  
Keyword(s):  
Eye Development ◽  
Zebrafish Embryos ◽  
Mineral Density

Fine structure of the retina of the giant scallop, Placopecten magellanicus

1984 ◽  
Vol 42 ◽  
pp. 312-313
Author(s):  
C.V.L. Powell
Keyword(s):  
Fine Structure ◽  
Electron Microscope ◽  
Light Intensity ◽  
Scanning Electron Microscope ◽  
Eye Development ◽  
Preliminary Observation ◽  
Columnar Epithelium ◽  
Placopecten Magellanicus ◽  
Environmental Light ◽  
Scanning Electron

The overall fine structure of the eye in Placopecten is similar to that of other scallops. The optic tentacle consists of an outer columnar epithelium which is modified into a pigmented iris and a cornea (Fig. 1). This capsule encloses the cellular lens, retina, reflecting argentea and the pigmented tapetum. The retina is divided into two parts (Fig. 2). The distal retina functions in the detection of movement and the proximal retina monitors environmental light intensity. The purpose of the present study is to describe the ultrastructure of the retina as a preliminary observation on eye development. This is also the first known presentation of scanning electron microscope studies of the eye of the scallop.

Pesticide and non-dioxin-like polychlorinated biphenyls (NDL-PCBs) residues in foodstuffs from Ismailia city, Egypt

2008 ◽  
pp. 1-9 ◽  
Author(s):  
N. Loutfy ◽  
M. Fuerhacker ◽  
C. Lesueur ◽  
M. Gartner ◽  
M. Tawfic Ahmed ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls

Mapping of accumulation of SeNPs in developing zebrafish embryos and larvae: a new method using SEM with energy dispersive X‐ray spectrometer

2017 ◽  
Vol 12 (7) ◽  
pp. 497-499
Author(s):  
Kalimuthu Kalishwaralal ◽  
Subhaschandrabose Jeyabharathi ◽  
Krishnan Sundar ◽  
Azhaguchamy Muthukumaran
Keyword(s):  
Energy Dispersive ◽  
New Method ◽  
Zebrafish Embryos ◽  
X Ray

Fatty Liver and Impaired Hepatic Metabolism Alters the Congener-Specific Distribution of Polychlorinated Biphenyls (PCBs) in Mice with a Liver-Specific Deletion of Cytochrome P450 Reductase

2020 ◽  
Author(s):  
Xueshu Li ◽  
Chun-Yun Zhang ◽  
Hans-Joachim Lehmler
Keyword(s):  
Cytochrome P450 ◽  
Polychlorinated Biphenyls ◽  
Fatty Liver ◽  
Partition Coefficients ◽  
Hepatic Metabolism ◽  
Gas Chromatography Mass Spectrometry ◽  
Cytochrome P450 Reductase ◽  
P450 Reductase ◽  
Specific Distribution ◽  
Specific Deletion

Polychlorinated biphenyls (PCBs) are persistent organic pollutants that are linked to adverse health outcomes. PCB tissue levels are determinants of PCB toxicity; however, it is unclear how factors, such as an altered metabolism and/or a fatty liver, affect PCB distribution in vivo. We determined the congener-specific disposition of PCBs in mice with a liver specific deletion of cytochrome P450 reductase (KO), a model of fatty liver with impaired hepatic metabolism, and wildtype (WT) mice. Male and female KO and WT mice were exposed orally to Aroclor 1254, a technical PCB mixture. PCBs were quantified in adipose, blood, brain and liver tissues by gas chromatography-mass spectrometry. PCB profiles and levels in tissues were genotype and sex dependent. PCB levels were higher in the liver from KO compared to WT mice. PCB profiles showed clear differences between tissues from the same exposure group. While experimental tissue : blood partition coefficients in KO and WT mice did not follow the trends predicted using a composition-based model, the agreement between experimental and calculated partition coefficients was still reasonable. Thus, a fatty liver and/or an impaired hepatic metabolism alter the distribution of PCBs in mice and the magnitude of the partitioning of PCBs from blood into tissues can be approximated using composition-based models.<br>

Determination of Polychlorinated Biphenyls (PCB) in Insulating Fluids

2017 ◽  
Vol 83 (11) ◽  
pp. 15-20
Author(s):  
E. S. Brodskii ◽  
◽  
A. A. Shelepchikov ◽  
G. A. Kalinkevich ◽  
E. Ya. Mir-Kadyrova ◽  
...  
Keyword(s):  
Polychlorinated Biphenyls
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