Expression of the transient receptor potential vanilloid 1 (TRPV1) in LNCaP and PC-3 prostate cancer cells and in human prostate tissue

2005 ◽  
Vol 515 (1-3) ◽  
pp. 20-27 ◽  
Author(s):  
María G. Sánchez ◽  
Ana M. Sánchez ◽  
Beatriz Collado ◽  
Sophie Malagarie-Cazenave ◽  
Nuria Olea ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Prostate Tissue ◽  
Human Prostate ◽  
Transient Receptor Potential Vanilloid ◽  
Prostate Cancer Cells ◽  
Human Prostate Tissue ◽  
Transient Receptor

scholarly journals Transient receptor potential melastatin 4 channel contributes to migration of androgen-insensitive prostate cancer cells

Oncotarget ◽  
2015 ◽  
Vol 6 (39) ◽  
pp. 41783-41793 ◽  
Author(s):  
Christian Holzmann ◽  
Sven Kappel ◽  
Tatiana Kilch ◽  
Marcus Martin Jochum ◽  
Sabine Katharina Urban ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Transient Receptor Potential ◽  
Receptor Potential ◽  
Prostate Cancer Cells ◽  
Transient Receptor Potential Melastatin ◽  
Transient Receptor

scholarly journals Therapeutic potential of TRPM8 antagonists in prostate cancer

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Marzia Di Donato ◽  
Carmine Ostacolo ◽  
Pia Giovannelli ◽  
Veronica Di Sarno ◽  
Isabel M. Gomez Monterrey ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Androgen Receptor ◽  
Cancer Cells ◽  
Cancer Cell ◽  
Transient Receptor Potential ◽  
Prostate Cancer Cell ◽  
Receptor Potential ◽  
Prostate Cancer Cells ◽  
Transient Receptor Potential Melastatin ◽  
Transient Receptor

AbstractTransient receptor potential melastatin-8 (TRPM8) represents an emerging target in prostate cancer, although its mechanism of action remains unclear. Here, we have characterized and investigated the effects of TRPM8 modulators in prostate cancer aggressiveness disclosing the molecular mechanism underlying their biological activity. Patch-clamp and calcium fluorometric assays were used to characterize the synthesized compounds. Androgen-stimulated prostate cancer-derived cells were challenged with the compounds and the DNA synthesis was investigated in a preliminary screening. The most effective compounds were then employed to inhibit the pro-metastatic behavior of in various PC-derived cells, at different degree of malignancy. The effect of the compounds was then assayed in prostate cancer cell-derived 3D model and the molecular targets of selected compounds were lastly identified using transcriptional and non-transcriptional reporter assays. TRPM8 antagonists inhibit the androgen-dependent prostate cancer cell proliferation, migration and invasiveness. They are highly effective in reverting the androgen-induced increase in prostate cancer cell spheroid size. The compounds also revert the proliferation of castrate-resistant prostate cancer cells, provided they express the androgen receptor. In contrast, no effects were recorded in prostate cancer cells devoid of the receptor. Selected antagonists interfere in non-genomic androgen action and abolish the androgen-induced androgen receptor/TRPM8 complex assembly as well as the increase in intracellular calcium levels in prostate cancer cells. Our results shed light in the processes controlling prostate cancer progression and make the transient receptor potential melastatin-8 as a ‘druggable’ target in the androgen receptor-expressing prostate cancers.

scholarly journals TRPM8 in prostate cancer cells: a potential diagnostic and prognostic marker with a secretory function?

2006 ◽  
Vol 13 (1) ◽  
pp. 27-38 ◽  
Author(s):  
Lei Zhang ◽  
Gregory J Barritt
Keyword(s):  
Prostate Cancer ◽  
Cancer Cells ◽  
Prognostic Marker ◽  
Cell Biology ◽  
Transient Receptor Potential ◽  
Sensory Nerve ◽  
Receptor Potential ◽  
Activation Mechanism ◽  
Prostate Cancer Cells ◽  
Genetic Interventions

During the past 5 years it has emerged that the transient receptor potential (TRP) family of Ca2+-and Na+-permeable channels plays a diverse and important role in cell biology and in pathology. One member of this family, TRPM8, is highly expressed in prostate cancer cells but the physiological and pathological functions of TRPM8 in these cells are not known. Here we address these questions, and the issue of whether or not TRPM8 is an effective diagnostic and prognostic marker in prostate cancer. TRPM8 is known to be activated by cool stimuli (17–25 °C) and cooling compounds such as menthol. The activation mechanism(s) involves voltage sensing of membrane potential, phosphatidylinositol 4,5-bisphosphate and Ca2+. In addition to prostate cancer cells, TRPM8 is expressed in sensory neurons where it acts as a sensor of cold. In prostate epithelial cells, expression of TRPM8 is regulated by androgen and is elevated in androgen-sensitive cancerous cells compared with normal cells. While there is some evidence that in prostate cancer cells Ca2+ and Na+ inflow through TRPM8 is necessary for survival and function, including secretion at the apical membrane, the function of TRPM8 in these cells is not really known. It may well differ from the role of TRPM8 as a cool sensor in sensory nerve cells. Androgen unresponsive prostate cancer is difficult to treat effectively and there are limited diagnostic and prognostic markers available. TRPM8 is a potential tissue marker in differential diagnosis and a potential prognostic marker for androgen-unresponsive and metastatic prostate cancer. As a consequence of its ability to convey Ca2+ and Na+ and its expression in only a limited number of cell types, TRPM8 is considered to be a promising target for pharmaceutical, immunological and genetic interventions for the treatment of prostate cancer.

scholarly journals The Antitumor Effect of Capsaicin on Prostate Cancer Cells Depends on AMPK Activation Through LKB1 Kinase and TRPV1 Receptor

2021 ◽  
Author(s):  
Belén G. Sánchez ◽  
Alicia Bort ◽  
Ines Diaz-Laviada
Keyword(s):  
Prostate Cancer ◽  
Cell Death ◽  
Cancer Cells ◽  
Cell Fate ◽  
Transient Receptor Potential ◽  
Therapeutic Option ◽  
Receptor Potential ◽  
Ampk Activation ◽  
Prostate Cancer Cells ◽  
Null Cell

Abstract Recently, natural compounds and their derivatives have been reported to have anti-cancer effects. Herein, we investigated the mechanism whereby the natural vanilloid capsaicin exerts anti-tumor effects on prostate cancer cells. We found that capsaicin activates the AMP-activated kinase (AMPK) and promotes cell death in the LKB1 expressing prostate cancer cell lines LNCaP and PC3 but not in the LKB1-null cell line DU-145. Capsaicin treatment stimulated LKB1 phosphorylation and activated AMPK in LKB1 expressing cells. In addition, overexpression of LKB1 by lentiviral infection in DU-145 cells, induced capsaicin-triggered AMPK activation and apoptosis while LKB1 silencing in LNCaP and PC3 cells, increased capsaicin-promoted cell death. Capsaicin-induced LKB1 phosphorylation, was dependent on the transient receptor potential cation channel subfamily V member 1 (TRPV1), since TRPV1 knocked down by shRNA, abolished LKB1 and AMPK phosphorylation and inhibited apoptosis. Altogether, our results show that capsaicin affect AMPK activity in an LKB1- and TRPV1-dependent fashion, linking TRPV1 with cell fate. These data also suggest that capsaicin may be a rational therapeutic option for prostate tumors.

scholarly journals TRP Channels in Human Prostate

2010 ◽  
Vol 10 ◽  
pp. 1597-1611 ◽  
Author(s):  
Carl Van Haute ◽  
Dirk De Ridder ◽  
Bernd Nilius
Keyword(s):  
Prostate Cancer ◽  
Transient Receptor Potential ◽  
Trp Channels ◽  
Current Knowledge ◽  
Receptor Potential ◽  
Human Prostate ◽  
Prostate Carcinogenesis ◽  
Potential Impact ◽  
Transient Receptor ◽  
Prostate Diseases

This review gives an overview of morphological and functional characteristics in the human prostate. It will focus on the current knowledge about transient receptor potential (TRP) channels expressed in the human prostate, and their putative role in normal physiology and prostate carcinogenesis. Controversial data regarding the expression pattern and the potential impact of TRP channels in prostate function, and their involvement in prostate cancer and other prostate diseases, will be discussed.

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