p38β-regulated induction of the heat shock response by carbon monoxide releasing molecule CORM-2 mediates cytoprotection in lung cells in vitro

The occurrence of heat-shock proteins (HSPs) in response to high temperature stress is a universal phenomenon in higher plants and has been well documented. However, in agriculturally important species, less is known about the expression of HSPs under natural environments. A review of the heat-shock response in wheat (Triticum aestivum L.) is presented and recent results on the expression of wheat HSPs under diurnal stress and field conditions are reported. In the field experiment, flag leaf blade temperatures were obtained and leaf blades collected for northern blot analysis using HSP 16.9 cDNA as a probe. Temperatures of leaf blades ranged from 32 to 35�C under the tested field conditions at New Deal near Lubbock, Texas. Messenger RNAs encoding a major class of low molecular weight HSPs, HSP 16.9, were detected in all wheat genotypes examined. The results suggested that HSPs are synthesised in response to heat stress under agricultural production, and furthermore, that HSPs are produced in wheats differing in geographic background. In the controlled growth chamber experiment, HSP expression in two wheat cultivars, Mustang (heat tolerant) and Sturdy (heat susceptible) were analysed to determine if wheat genotypes differing in heat tolerance differ in in vitro HSP synthesis (translatable HSP mRNAs) under a chronic, diurnal heat-stress regime. Leaf tissues were collected from seedlings over a time-course and poly (A)+RNAs were isolated for in vitro translation and 2-D gel electrophoresis. The protein profiles shown in the 2-D gel analysis revealed that there were not only quantitative differences of individual HSPs between these two wheat lines, but also some unique HSPs which were only found in the heat tolerant line. This data provides evidence of a correlation between HSP synthesis and heat tolerance in wheat under a simulated field environment and suggests that further genetic analysis of HSPs in a segregating population is worthy of investigation. In conclusion, the results of this study provide an impetus for the investigation of the roles of HSP genes in heat tolerance in wheat.

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P69 Investigating the effects of pharmacological up-regulation of the heat shock response in an in-vitro model of sporadic inclusion body myositis

Neuromuscular Disorders ◽

10.1016/s0960-8966(12)70077-9 ◽

2012 ◽

Vol 22 ◽

pp. S26

Author(s):

M. Ahmed ◽

A. Miller ◽

M.G. Hanna ◽

L. Greensmith

Keyword(s):

Heat Shock ◽

Inclusion Body ◽

Heat Shock Response ◽

Inclusion Body Myositis ◽

In Vitro Model ◽

Shock Response ◽

Sporadic Inclusion Body Myositis ◽

Vitro Model

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PONM17 Augmentation of the heat shock response in an in vitro model of sporadic inclusion body myositis

Journal of Neurology Neurosurgery & Psychiatry ◽

10.1136/jnnp.2010.226340.190 ◽

2010 ◽

Vol 81 (11) ◽

pp. e64-e64

Author(s):

A. Miller ◽

M. Ahmed ◽

M. G. Hanna ◽

L. Greensmith

Keyword(s):

Heat Shock ◽

Inclusion Body ◽

Heat Shock Response ◽

Inclusion Body Myositis ◽

In Vitro Model ◽

Shock Response ◽

Sporadic Inclusion Body Myositis ◽

Vitro Model

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Caloric restriction induces heat shock response and inhibits B16F10 cell tumorigenesis both in vitro and in vivo

Aging ◽

10.18632/aging.100732 ◽

2015 ◽

Vol 7 (4) ◽

pp. 233-240 ◽

Cited By ~ 3

Author(s):

Marta G. Novelle ◽

Ashley Davis ◽

Nathan L. Price ◽

Ahmed Ali ◽

Stefanie Fürer-Galvan ◽

...

Keyword(s):

Heat Shock ◽

Caloric Restriction ◽

Heat Shock Response ◽

B16f10 Cell ◽

Shock Response

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Abstract 16758: Cardiosphere Derived Cells From Pediatric End-Stage Heart Failure Patients Have Enhanced Functional Activity Due to the Heat Shock Response Regulating the Secretome

Circulation ◽

10.1161/circ.130.suppl_2.16758 ◽

2014 ◽

Vol 130 (suppl_2) ◽

Author(s):

Sudhish Sharma ◽

Rachana MIshra ◽

David Simpson ◽

Evan J Colletti ◽

savitha Deshmukh ◽

...

Keyword(s):

Stem Cells ◽

Heat Shock ◽

Functional Recovery ◽

Heat Shock Response ◽

Functional Activity ◽

Loss Of Function ◽

Injured Myocardium ◽

Shock Response ◽

End Stage

Rationale: Results from pre-clinical studies and a Phase I clinical study demonstrates promising functional recovery by adult cardiosphere derived cells (CDCs) to repair damaged myocardium. We have previously demonstrated that age regulates the functional recovery of neonatal CDCs due to their more effective secretome. However, how advanced failure pediatric hearts regulate their derived CDC’s functional activity is still unknown. Objective: Our aim was to compare the functional potency between pediatric CDCs derived from normal myocardium of congenital heart disease (CHD) patients and from dysfunctional myocardium of pediatric end stage failing heart (ESHF) patients and determine the mechanisms involved. Methods and Results: ESHF derived hCDCs had a higher number of cardiac stem cells expressing c-kit+, Islet-1+, and Sca-1+. When transplanted in an infracted rodent model, ESHF derived hCDCs had a significantly higher preservation of ventricular function, prevented adverse remodeling, and enhanced angiogenesis when compared with CHD derived CDCs. The superior functional recovery of the ESHF derived hCDCs was mediated in part by increased SDF-1α and VEGF-A secretion which recruited more endogenous stem cells and proliferation of cardiomyocytes. The mechanism for the superior secretome is due to the heat shock response (HSR) which is supported by three lines of evidence. First, gain of function studies demonstrated that the HSR induced the low functioning CHD derived CDCs to significantly recover the injured myocardium to the same extent as ESHF derived CDCs. Secondly, loss-of function studies targeting the HSR down regulated the ability of the ESHF derived CDCs to functionally recover the injured myocardium. Finally, the HSR alone increased the number of the c-kit+ CSCs both in vitro and in vivo. Conclusion: These findings suggest that the HSR enhances the functional activity of ESHF derived CDCs by regulating their secretome.

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Monoterpenes induce the heat shock response in Arabidopsis

Zeitschrift für Naturforschung C ◽

10.1515/znc-2017-0116 ◽

2018 ◽

Vol 73 (5-6) ◽

pp. 177-184 ◽

Cited By ~ 3

Author(s):

Masakazu Hara ◽

Naoya Yamauchi ◽

Yoshiki Sumita

Keyword(s):

Heat Shock ◽

Heat Shock Protein ◽

Essential Oils ◽

Heat Tolerance ◽

Heat Shock Response ◽

Small Heat Shock Protein ◽

Shock Response ◽

Reporter Gene System ◽

Heat Shock Protein Genes

Abstract Monoterpenes are common constituents of essential oils produced by plants. Although it has been reported that monoterpenes enhanced the heat tolerance of plants, the mechanism has not been elucidated. Here, we tested whether 13 monoterpenes promoted the heat shock response (HSR) in Arabidopsis. To assess the HSR-inducing activity of monoterpenes, we produced transgenic Arabidopsis, which has the β-glucuronidase gene driven by the promoter of a small heat shock protein (HSP17.6C-CI) gene. Results indicated that two monocyclic and four bicyclic monoterpenes showed HSR-inducing activities using the reporter gene system. In particular, (−)-perillaldehyde, which is a monocyclic monoterpene, demonstrated the most potent HSR-inducing activity. (−)-Perillaldehyde significantly inhibited the reduction of chlorophyll content by heat shock in Arabidopsis seedlings. Our previous study indicated that chemical HSR inducers such as geldanamycin and sanguinarine inhibited the activity of plant chaperones in vitro. (−)-Perillaldehyde also inhibited chaperone activity, indicating that it might promote the expression of heat shock protein genes by inhibiting chaperones in the plant cell.

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