Acyclovir lipid nanocapsules gel for oromucosal delivery: A preclinical evidence of efficacy in the chicken pouch membrane model

Abstract An analytical membrane model is used to study how wheel imperfections are converted into radial force variation of the tire-wheel assembly. This model indicates that the radial run-out of the rim generates run-out of the tire-wheel assembly at slightly less than the one to one ratio that was expected. Lateral run-out of the rim is found to generate radial run-out of the tire-wheel assembly at a ratio that is dependent on the tire design and the wheel width. Finite element studies of a production tire validate and quantify the results of the membrane model. Experiments using a specially constructed precision wheel demonstrate the behavior predicted by the models. Finally, a population of production tires and wheels show that the lateral run-out of the rims contribute a significant portion to the assembly radial force variation. These findings might be used to improve match-mounting results by taking lateral rim run-out into account.

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Folic acid and zinc inhibit angiogenesis in chicken chorioallontoic membrane model via angiogenic factor genes

GULHANE MEDICAL JOURNAL ◽

10.26657/gulhane.00024 ◽

2018 ◽

Vol 60 (3) ◽

pp. 67 ◽

Cited By ~ 1

Author(s):

Şefik Güran ◽

Zehra Dilşad Çoban ◽

Orhan Fermanlı ◽

Emre Aydıngöz ◽

Emre İpek

Keyword(s):

Folic Acid ◽

Membrane Model ◽

Angiogenic Factor

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Faculty Opinions recommendation of Molecular dynamics simulations of large integral membrane proteins with an implicit membrane model.

Faculty Opinions – Post-Publication Peer Review of the Biomedical Literature ◽

10.3410/f.1031397.363724 ◽

2006 ◽

Author(s):

D Peter Tieleman

Keyword(s):

Molecular Dynamics ◽

Membrane Proteins ◽

Molecular Dynamics Simulations ◽

Integral Membrane Proteins ◽

Membrane Model ◽

Dynamics Simulations

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Kinetic Membrane Model of Outer Hair Cells

Biophysical Journal ◽

10.1016/j.bpj.2020.11.017 ◽

2021 ◽

Vol 120 (1) ◽

pp. 122-132

Author(s):

Kuni H. Iwasa

Keyword(s):

Hair Cells ◽

Outer Hair Cells ◽

Membrane Model

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Biophysical characterization of polymyxin b interaction with LPS aggregates and membrane model systems

Biopolymers ◽

10.1002/bip.22095 ◽

2012 ◽

Vol 98 (4) ◽

pp. 338-344 ◽

Cited By ~ 69

Author(s):

Marco M. Domingues ◽

Rita G. Inácio ◽

José M. Raimundo ◽

Miguel Martins ◽

Miguel A. R. B. Castanho ◽

...

Keyword(s):

Polymyxin B ◽

Model Systems ◽

Membrane Model ◽

Biophysical Characterization

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New In Vitro Coculture Model for Evaluating Intestinal Absorption of Different Lipid Nanocapsules

Pharmaceutics ◽

10.3390/pharmaceutics13050595 ◽

2021 ◽

Vol 13 (5) ◽

pp. 595

Author(s):

Norraseth Kaeokhamloed ◽

Emillie Roger ◽

Jérôme Béjaud ◽

Nolwenn Lautram ◽

Florence Manero ◽

...

Keyword(s):

Surface Modification ◽

Intestinal Absorption ◽

Oral Absorption ◽

Membrane Integrity ◽

Microvascular Endothelial Cell ◽

Absorption Mechanism ◽

Apparent Permeability ◽

Lipid Nanocapsules ◽

Coculture Model

Standard models used for evaluating the absorption of nanoparticles like Caco-2 ignore the presence of vascular endothelium, which is a part of the intestinal multi-layered barrier structure. Therefore, a coculture between the Caco-2 epithelium and HMEC-1 (Human Microvascular Endothelial Cell type 1) on a Transwell® insert has been developed. The model has been validated for (a) membrane morphology by transmission electron microscope (TEM); (b) ZO-1 and β-catenin expression by immunoassay; (c) membrane integrity by trans-epithelial electrical resistance (TEER) measurement; and (d) apparent permeability of drugs from different biopharmaceutical classification system (BCS) classes. Lipid nanocapsules (LNCs) were formulated with different sizes (55 and 85 nm) and surface modifications (DSPE-mPEG (2000) and stearylamine). Nanocapsule integrity and particle concentration were monitored using the Förster resonance energy transfer (FRET) technique. The result showed that surface modification by DSPE-mPEG (2000) increased the absorption of 55-nm LNCs in the coculture model but not in the Caco-2. Summarily, the coculture model was validated as a tool for evaluating the intestinal absorption of drugs and nanoparticles. The new coculture model has a different LNCs absorption mechanism suggesting the importance of intestinal endothelium and reveals that the surface modification of LNCs can modify the in vitro oral absorption.

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Investigation on Heat and Mass transfer in a Dialyzer Membrane Model for the Development of Dialysate Temperature Controller

Proceedings of the 2018 5th International Conference on Bioinformatics Research and Applications - ICBRA '18 ◽

10.1145/3309129.3309135 ◽

2018 ◽

Author(s):

Mohamed Haroon Abdul Jabbar ◽

S. Anandan Shanmugam ◽

Poi Sim Khiew

Keyword(s):

Mass Transfer ◽

Heat And Mass Transfer ◽

Membrane Model ◽

Temperature Controller ◽

Dialysate Temperature

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Antiangiogenic potential of Jatropha curcas latex in the chick chorioallantoic membrane model

Scientia Medica ◽

10.15448/1980-6108.2019.1.32157 ◽

2019 ◽

Vol 29 (1) ◽

pp. 32157

Author(s):

Luciane Madureira Almeida ◽

Elisa Flávia Luiz Cardoso Bailão ◽

Illana Reis Pereira ◽

Fabrício Alves Ferreira ◽

Patrícia Lima D'Abadia ◽

...

Keyword(s):

Thermal Stability ◽

Jatropha Curcas ◽

Physicochemical Characterization ◽

Chorioallantoic Membrane ◽

Angiogenesis Inhibitor ◽

Negative Control ◽

New Drugs ◽

Membrane Model ◽

Chick Chorioallantoic Membrane

AIMS: To perform a physicochemical and phytochemical characterization of Jatropha curcas latex and to investigate its antiangiogenic potential. METHODS: We performed an initial physicochemical characterization of J. curcas latex using thermal gravimetric analyses and Fourier Transform Infrared spectroscopy. After that, phenols, tannins and flavonoids were quantified. Finally, the potential of J. curcas latex to inhibit angiogenesis was evaluated using the chick chorioallantoic membrane model. Five groups of 20 fertilized chicken eggs each had the chorioallantoic membrane exposed to the following solutions: (1) water, negative control; (2) dexamethasone, angiogenesis inhibitor; (3) Regederm®, positive control; (4) 25% J. curcas latex diluted in water; (5) 50% J. curcas latex diluted in water; and (6) J. curcas crude latex. Analysis of the newly-formed vascular net was made through captured images and quantification of the number of pixels. Histological analyses were performed to evaluate the inflammation, neovascularization, and hyperemia parameters. The results were statically analyzed with a significance level set at p ˂0.05.RESULTS: Physicochemical characterization showed that J. curcas latex presented a low amount of cis-1.4-polyisoprene, which reduced its elasticity and thermal stability. Phytochemical analyses of J. curcas latex identified a substantial amount of phenols, tannins, and flavonoids (51.9%, 11.8%, and 0.07% respectively). Using a chick chorioallantoic membrane assay, we demonstrated the antiangiogenic potential of J. curcas latex. The latex induced a decrease in the vascularization of the membranes when compared with neutral and positive controls (water and Regederm®). However, when compared with the negative control (dexamethasone), higher J. curcas latex concentrations showed no significant differences.CONCLUSIONS: J. curcas latex showed low thermal stability, and consisted of phenols, tannins, and flavonoids, but little or no rubber. Moreover, this latex demonstrated a significant antiangiogenic activity on a chick chorioallantoic membrane model. The combination of antimutagenic, cytotoxic, antioxidant and antiangiogenic properties makes J. curcas latex a potential target for the development of new drugs.

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