Sequential bioethanol and biogas production from sugarcane bagasse based on high solids fed-batch SSF

Energy ◽  
2015 ◽  
Vol 90 ◽  
pp. 1199-1205 ◽  
Author(s):  
Yunyun Liu ◽  
Jingliang Xu ◽  
Yu Zhang ◽  
Zhenhong Yuan ◽  
Minchao He ◽  
...  
2015 ◽  
Vol 211 ◽  
pp. 5-9 ◽  
Author(s):  
Yunyun Liu ◽  
Jingliang Xu ◽  
Yu Zhang ◽  
Zhenhong Yuan ◽  
Jun Xie

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 3839-3849 ◽  
Author(s):  
Yunyun Liu ◽  
Yunqi Cao ◽  
Qiang Yu ◽  
Jingliang Xu ◽  
Zhenhong Yuan

Complementary enzymes can considerably enhance the hydrolysis effectiveness of cellulase. The influence of hemicellulase supplementation on high solids saccharification of alkali-pretreated sugarcane bagasse was assessed. Hemicellulase addition of 1200 IU/g substrate with cellulase loading of 10 FPU/g substrate achieved high sugars yield with glucose and xylose conversion efficiency of 95.4% and 87.4%, respectively. To further improve the substrate conversion efficiency based on high sugars production, fed-batch hydrolysis was employed with high solids loading of 20% (w/v) to 25% (w/v). After 96 h hydrolysis with 25% solids loading at cellulase and hemicellulase loading of 20 FPU/g and 1200 IU/g substrate, respectively, the obtained highest total sugars was 242 g/L, with glucose and xylose conversion efficiencies of 98.6% and 94.9%, respectively. An increase in substrate digestibility upon supplementation of mixture enzymes with high sugars production can be realized in high solids fed-batch system with proper cellulase and hemicellulase synergism.


Water ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Aiban Abdulhakim Saeed Ghaleb ◽  
Shamsul Rahman Mohamed Kutty ◽  
Gasim Hayder Ahmed Salih ◽  
Ahmad Hussaini Jagaba ◽  
Azmatullah Noor ◽  
...  

Man-made organic waste leads to the rapid proliferation of pollution around the globe. Effective bio-waste management can help to reduce the adverse effects of organic waste while contributing to the circular economy at the same time. The toxic oily-biological sludge generated from oil refineries’ wastewater treatment plants is a potential source for biogas energy recovery via anaerobic digestion. However, the oily-biological sludge’s carbon/nitrogen (C/N) ratio is lower than the ideal 20–30 ratio required by anaerobic digestion technology for biogas production. Sugarcane bagasse can be digested as a high C/N co-substrate while the oily-biological sludge acts as a substrate and inoculum to improve biogas production. In this study, the best C/N with co-substrate volatile solids (VS)/inoculum VS ratios for the co-digestion process of mixtures were determined empirically through batch experiments at temperatures of 35–37 °C, pH (6–8) and 60 rpm mixing. The raw materials were pre-treated mechanically and thermo-chemically to further enhance the digestibility. The best condition for the sugarcane bagasse delignification process was 1% (w/v) sodium hydroxide, 1:10 solid-liquid ratio, at 100 °C, and 150 rpm for 1 h. The results from a 33-day batch anaerobic digestion experiment indicate that the production of biogas and methane yield were concurrent with the increasing C/N and co-substrate VS/inoculum VS ratios. The total biogas yields from C/N 20.0 with co-substrate VS/inoculum VS 0.06 and C/N 30.0 with co-substrate VS/inoculum VS 0.18 ratios were 2777.0 and 9268.0 mL, respectively, including a methane yield of 980.0 and 3009.3 mL, respectively. The biogas and methane yield from C/N 30.0 were higher than the biogas and methane yields from C/N 20.0 by 70.04 and 67.44%, respectively. The highest biogas and methane yields corresponded with the highest C/N with co-substrate VS/inoculum VS ratios (30.0 and 0.18), being 200.6 mL/g VSremoved and 65.1 mL CH4/g VSremoved, respectively.


2021 ◽  
Vol 14 (1) ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu

Abstract Background Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 °C for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization. Conclusion This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.


2020 ◽  
Author(s):  
Lin Dai ◽  
Tian Huang ◽  
Kankan Jiang ◽  
Xin Zhou ◽  
Yong Xu

Abstract Background: Pretreatment is the key step for utilizing lignocellulosic biomass, which can extract cellulose from lignin and disrupt its recalcitrant crystalline structure to allow much more effective enzymatic hydrolysis; and organic acids pretreatment with dual benefic for generating xylooligosaccharides and boosting enzymatic hydrolysis has been widely used in adding values to lignocellulose materials. In this work, furoic acid, a novel recyclable organic acid as catalyst, was employed to pretreat sugarcane bagasse to recover the xylooligosaccharides fraction from hemicellulose and boost the subsequent cellulose saccharification. Results: The FA-assisted hydrolysis of sugarcane bagasse using 3% furoic acid at 170 oC for 15 min resulted in the highest xylooligosaccharides yield of 45.6%; subsequently, 83.1 g/L of glucose was harvested by a fed-batch operation with a solid loading of 15%. Overall, a total of 120 g of xylooligosaccharides and 335 g glucose could be collected from 1000 g sugarcane bagasse starting from the furoic acid pretreatment. Furthermore, furoic acid can be easily recovered by cooling crystallization.Conclusion: This work put forward a novel furoic acid pretreatment method to convert sugarcane bagasse into xylooligosaccharides and glucose, which provides a strategy that the sugar and nutraceutical industries can be used to reduce the production cost. The developed process showed that the yields of xylooligosaccharides and byproducts were controllable by shortening the reaction time; meanwhile, the recyclability of furoic acid also can potentially reduce the pretreatment cost and potentially replace the traditional mineral acids pretreatment.


2018 ◽  
Vol 119 ◽  
pp. 191-197 ◽  
Author(s):  
Javier Ulises Hernández-Beltrán ◽  
Héctor Hernández-Escoto

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