Rapid thermal-acid hydrolysis of spiramycin by silicotungstic acid under microwave irradiation

Abstract A qualitative and quantitative gravimetric procedure for Polysorbate 80 in pickle products involves acid hydrolysis of the comminuted sample, saponification, extraction of the glycol portion of the molecule with chloroform, and removal of interfering fatty acids with petroleum ether. The glycol is finally precipitated with silicotungstic acid, determined gravimetrically, and identified by infrared spectroscopy. Recoveries from commercially prepared pickle relish and dill pickles containing 0.01 to 0.02% Polysorbate 80 ranged from 80 to 98.5%

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Salt-assisted acid hydrolysis of chitosan to oligomers under microwave irradiation

Carbohydrate Research ◽

10.1016/j.carres.2005.06.028 ◽

2005 ◽

Vol 340 (13) ◽

pp. 2150-2153 ◽

Cited By ~ 73

Author(s):

Ronge Xing ◽

Song Liu ◽

Huahua Yu ◽

Zhanyong Guo ◽

Pibo Wang ◽

...

Keyword(s):

Microwave Irradiation ◽

Acid Hydrolysis ◽

Hydrolysis Of

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Regulation of acid hydrolysis of sucrose in acid lime juice sac cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1991.810108.x ◽

1991 ◽

Vol 81 (1) ◽

pp. 51-54 ◽

Cited By ~ 1

Author(s):

Ed Echeverria

Keyword(s):

Acid Hydrolysis ◽

Lime Juice ◽

Acid Lime ◽

Hydrolysis Of

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DETERMINATION OF MICRO-AMOUNTS OF 5β-PREGNAN-3α-OL-20-ONE IN URINE USING INFRARED-SPECTROMETRY

Acta Endocrinologica ◽

10.1530/acta.0.0410234 ◽

1962 ◽

Vol 41 (2) ◽

pp. 234-246 ◽

Cited By ~ 5

Author(s):

H. J. van der Molen

Keyword(s):

Infrared Spectrum ◽

Quantitative Determination ◽

Acid Hydrolysis ◽

Chromatographic Separation ◽

Pure Form ◽

Infrared Spectrometry ◽

Hydrolysis Of

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

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