Gravimetric Procedure for the Quantitation and Identification of Polysorbate 80 in Pickle Products

Abstract A qualitative and quantitative gravimetric procedure for Polysorbate 80 in pickle products involves acid hydrolysis of the comminuted sample, saponification, extraction of the glycol portion of the molecule with chloroform, and removal of interfering fatty acids with petroleum ether. The glycol is finally precipitated with silicotungstic acid, determined gravimetrically, and identified by infrared spectroscopy. Recoveries from commercially prepared pickle relish and dill pickles containing 0.01 to 0.02% Polysorbate 80 ranged from 80 to 98.5%

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Method for Isolation and Detection of Dioctyl Phthalate from Milk Lipids

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/50.3.646 ◽

1967 ◽

Vol 50 (3) ◽

pp. 646-650

Author(s):

J Cerbulis ◽

J S Ard

Keyword(s):

Fatty Acids ◽

Infrared Spectroscopy ◽

Petroleum Ether ◽

Petroleum Ether Extract ◽

Dairy Farm ◽

Dioctyl Phthalate ◽

Ether Extract ◽

Alumina Column ◽

Milk Lipids ◽

Layer Chromatography

Abstract A method has been developed to isolate dioctyl phthalate (DOP, di-(2-ethyIhexyI) phthalate) from milk. Milk from an individual dairy farm was dialyzed and evaporated to dryness, and the dry residue was extracted with petroleum ether. The petroleum ether extract of dried milk was chromatographed on an alumina column as described by Hanahan. The fraction containing free fatty acids and DOP was separated by thin layer chromatography (TLC). DOP was distinguished from other phthalates by TLC and infrared spectroscopy

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1-(O-β-Glucosaminyl)-2,3-diglyceride in Bacillus megaterium

Biochemical Journal ◽

10.1042/bj1260499 ◽

1972 ◽

Vol 126 (3) ◽

pp. 499-502 ◽

Cited By ~ 9

Author(s):

P. J. R. Phizackerley ◽

J. C. MacDougall ◽

R. A. Moore

Keyword(s):

Fatty Acids ◽

Acid Hydrolysis ◽

Bacillus Megaterium ◽

Total Lipid ◽

Alkaline Hydrolysis ◽

Partial Acid Hydrolysis ◽

Molar Proportion ◽

Ester Linkage ◽

Hydrolysis Of

1. A lipid that contains glucosamine but not phosphorus has been isolated from Bacillus megaterium. It constitutes about 5% of the total lipid glucosaminide in this organism and can be distinguished chromatographically from 2′-(O-β-glucosaminyl)phosphatidylglycerol and 3′-(O-β-glucosaminyl)phosphatidylglycerol, which are also present. 2. The lipid contains glycerol, fatty acids and glucosamine in the molar proportion 1:2:1. The fatty acids are bound by an ester linkage and are similar to those found in other lipids of this organism. Partial acid hydrolysis or alkaline hydrolysis of the lipid yields 1-(O-β-glucosaminyl)glycerol and degradation with nitrite yields 2,5-anhydromannose and diglyceride. 3. The lipid has been identified as 1-(O-β-glucosaminyl)-2,3-diglyceride.

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THE STRUCTURE OF A SYNTHETIC GLUCAN: I. GENERAL STRUCTURAL FEATURES

Canadian Journal of Chemistry ◽

10.1139/v62-182 ◽

1962 ◽

Vol 40 (6) ◽

pp. 1196-1200 ◽

Cited By ~ 13

Author(s):

G. G. S. Dutton ◽

A. M. Unrau

Keyword(s):

Petroleum Ether ◽

Acid Hydrolysis ◽

Direct Evidence ◽

Periodate Oxidation ◽

Structural Features ◽

Degree Of Polymerization ◽

Molar Ratio ◽

Partial Hydrolysis ◽

Large Numbers ◽

Hydrolysis Of

Only D-glucose was obtained on acid hydrolysis of the glucan. Periodate oxidation released formaldehyde, which was believed to arise from C6 of D-glucofuranose units. From the additional formaldehyde liberated from the borohydride-reduced glucan the degree of polymerization was estimated to be about 165. Complete hydrolysis of the derived polyalcohol gave glycerol, erythritol, D-glucose, and D-xylose. Partial hydrolysis gave glycerol, erythritol, and at least seven non-reducing oligosaccharides. Direct evidence for the existence of relatively large numbers of 1 → 6 and 1 → 4 linkages was found, together with smaller numbers of 1 → 2 linkages. The methylated glucan was freely soluble in chloroform – petroleum ether (5:95), and hydrolysis gave tetra, tri, di, and mono-O-methyl-D-glucoses in a 6:6:3:1 molar ratio.

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Rapid thermal-acid hydrolysis of spiramycin by silicotungstic acid under microwave irradiation

Environmental Pollution ◽

10.1016/j.envpol.2019.02.074 ◽

2019 ◽

Vol 249 ◽

pp. 36-44 ◽

Cited By ~ 3

Author(s):

Zheng Chen ◽

Xiaomin Dou ◽

Yu Zhang ◽

Min Yang ◽

Dongbin Wei

Keyword(s):

Microwave Irradiation ◽

Acid Hydrolysis ◽

Silicotungstic Acid ◽

Thermal Acid Hydrolysis ◽

Hydrolysis Of

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Regulation of acid hydrolysis of sucrose in acid lime juice sac cells

Physiologia Plantarum ◽

10.1034/j.1399-3054.1991.810108.x ◽

1991 ◽

Vol 81 (1) ◽

pp. 51-54 ◽

Cited By ~ 1

Author(s):

Ed Echeverria

Keyword(s):

Acid Hydrolysis ◽

Lime Juice ◽

Acid Lime ◽

Hydrolysis Of

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DETERMINATION OF MICRO-AMOUNTS OF 5β-PREGNAN-3α-OL-20-ONE IN URINE USING INFRARED-SPECTROMETRY

Acta Endocrinologica ◽

10.1530/acta.0.0410234 ◽

1962 ◽

Vol 41 (2) ◽

pp. 234-246 ◽

Cited By ~ 5

Author(s):

H. J. van der Molen

Keyword(s):

Infrared Spectrum ◽

Quantitative Determination ◽

Acid Hydrolysis ◽

Chromatographic Separation ◽

Pure Form ◽

Infrared Spectrometry ◽

Hydrolysis Of

ABSTRACT A procedure for the quantitative determination of 5β-pregnan-3α-ol-20-one in urine is described. After acid hydrolysis of the pregnanolone-conjugates in urine, the free steroids are extracted with toluene. Pregnanolone is isolated in a pure form as its acetate; after chromatographic separation of the free steroids on alumina, the fraction containing pregnanolone is acetylated and rechromatographed on alumina. Quantitative determination of the isolated pregnanolone-acetate is carried out with the aid of the infrared spectrum recorded by a micro KBr-wafermethod. The reliability of the method under various conditions is discussed under the headings, specificity, accuracy, precision and sensitivity. It is possible to determine 30–40 μg pregnanolone in a 24-hours urine portion with a precision of 25%.

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