Multi-omics analyses on the response mechanisms of ‘Shine Muscat’ grapevine to low degree of excess copper stress (Low-ECS)

AbstractThe aim of the study was to investigate the potential beneficial effects of exogenous application of methyl jasmonate (MeJa) and phenylurea cytokinin (4PU-30) on zucchini (Cucurbita pepo L.) cotyledons subjected to high temperature (HT) and combined HT plus excess copper (Cu2+) stress. Compared to HT stressed zucchini cotyledons, exposure to excess Cu2+ prior to HT treatment inhibited the growth, decreased superoxide dismutase (SOD), glutathione peroxidase (GPx) and glutathione reductase (GR) activities, increased glutathione-Stransferase (GST) and peroxidase (Px) total activities, and induced new enzyme isoforms (GST4, GST8, and Px4). The effect of MeJa on the double-stressed plants was to increase SOD, GPx and Px total activities and stimulate the activity of GST8, GST9, GST10, SOD5, Px3 and Px8 isoenzymes. In addition to SOD, GPx and Px, 4PU-30 increased GST total activity, up-regulated Px1 and GR1, and had a higher stimulatory effect on SOD5, Px3, GPx3, GST9 and GST10 activities. Copper, 4PU-30 and MeJa treatments changed the abundance and pattern (MeJa) of soluble zucchini polypeptides in a specific manner. MeJa and especially 4PU-30 applied alone, or in combination, stimulated the growth of later HT-treated cotyledons. 4PU-30 counteracted better than MeJa in the HT+heavy metal-caused inhibition of the growth. The data indicated that both MeJa and 4PU- 30 may protect the zucchini plants from high temperature and combined high temperature plus excess copper stress, but 4PU-30 was more effective; the protective effect was related to the changes in the gene expression, total antioxidant enzyme activities and the activity of individual isoforms of the enzymes.

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Excess copper induces structural changes in cultured photosynthetic soybean cells

Functional Plant Biology ◽

10.1071/fp06174 ◽

2006 ◽

Vol 33 (11) ◽

pp. 1001 ◽

Cited By ~ 18

Author(s):

María Bernal ◽

Pilar Sánchez-Testillano ◽

María del Carmen Risueño ◽

Inmaculada Yruela

Keyword(s):

Cell Wall ◽

Structural Changes ◽

Copper Tolerance ◽

Copper Stress ◽

Induced Response ◽

Cytoplasmic Vacuole ◽

Cell Nuclei ◽

Short Term ◽

Excess Copper

Soybean [Glycine max (L.) Merr.] cell suspensions have the capacity to develop tolerance to excess copper, constituting a convenient system for studies on the mechanisms of copper tolerance. The functional cell organisation changes observed in these cell cultures after both short-term (stressed cells) and long-term (acclimated cells) exposure to 10 μm CuSO4 are reported from structural, cytochemical and microanalytical approaches. Cells grown in the presence of 10 μm CuSO4 shared some structural features with untreated cells, such as: (i) a large cytoplasmic vacuole, (ii) chloroplasts along the thin layer of cytoplasm, (iii) nucleus in a peripheral location exhibiting circular-shaped nucleolus and a decondensed chromatin pattern, and (iv) presence of Cajal bodies in the cell nuclei. In addition, cells exposed to 10 μm CuSO4 exhibited important differences compared with untreated cells: (i) chloroplasts displayed rounded shape and smaller size with denser-structured internal membranes, especially in copper-acclimated cells; (ii) no starch granules were found within chloroplasts; (iii) the cytoplasmic vacuole was larger, especially after long-term copper exposure; (iv) the levels of citrate and malate increased. Extracellular dark-coloured deposits with high copper content attached at the outer surface of the cell wall were observed only in cells exposed to a short-term copper stress. Structural cell modifications, mainly affecting chloroplasts, accompanied the short-term copper-induced response and were maintained as stable characters during the period of adaptation to excess copper. Vacuolar changes accompanied the long-term copper response. The results indicate that the first response of soybean cells to excess copper prevents its entry into the cell by immobilising it in the cell wall, and after an adaptive period, acclimation to excess copper may be mainly due to vacuolar sequestration.

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Hydrilla verticillata employs two different ways to affect DNA methylation under excess copper stress

Aquatic Toxicology ◽

10.1016/j.aquatox.2017.10.007 ◽

2017 ◽

Vol 193 ◽

pp. 97-104 ◽

Cited By ~ 11

Author(s):

Danlu Shi ◽

Kai Zhuang ◽

Yan Xia ◽

Changhua Zhu ◽

Chen Chen ◽

...

Keyword(s):

Dna Methylation ◽

Hydrilla Verticillata ◽

Copper Stress ◽

Excess Copper

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Effects of proline on copper transport in rice seedlings under excess copper stress

Plant Science ◽

10.1016/j.plantsci.2003.08.015 ◽

2004 ◽

Vol 166 (1) ◽

pp. 103-111 ◽

Cited By ~ 67

Author(s):

Chien-Teh Chen ◽

Tsai-Huei Chen ◽

Kuo-Fu Lo ◽

Chih-Yu Chiu

Keyword(s):

Copper Transport ◽

Copper Stress ◽

Rice Seedlings ◽

Excess Copper

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Copper stress affect seed germination and seedling establishment of Schinus terebinthifolia Raddi

Hoehnea ◽

10.1590/2236-8906-50/2020 ◽

2020 ◽

Vol 47 ◽

Author(s):

Matheus Casarini Siqueira ◽

Armando Reis Tavares ◽

José Marcos Barbosa ◽

Nelson Augusto dos Santos Junior

Keyword(s):

Seed Germination ◽

Seedling Establishment ◽

Environmental Problem ◽

Copper Stress ◽

Control Treatment ◽

Tree Seedlings ◽

Excess Copper ◽

Pepper Tree ◽

Mean Germination Time ◽

Brazilian Pepper

ABSTRACT Pollution by the discharge of copper in the environment due to anthropogenic, industrial, and automobile activities is an environmental problem. Copper is also widely used in different forms as fertilizers and fungicides. Here, we aimed to evaluate seed germination and the establishment of Schinus terebinthifolia Raddi, popularly known as ‘Brazilian pepper tree’ seedlings submitted to different concentrations of copper. Five treatments were used, with four replicates and 20 seeds per replicate, totaling 400 seeds. Cu (CuSO4.5H2O) was added to the substrate at doses of 0; 60; 760; 2,100 or 10,000 mg kg-1. The variables percentage, speed, and mean germination time, as well as establishment of seedlings, were analyzed. Cu doses of 2,100 or 10,000 mg kg-1 inhibited seedling formation. Cu concentration of 10,000 mg kg-1 reduced the final values of germination and speed germination to about one third of those obtained in the control treatment. Compared to germination, establishment of seedlings was more affected by excess copper for the S. terebinthifolia. The species tolerated doses up to 760 mg kg-1 copper during seedling establishment, which indicates that it is a candidate for environmental restoration programs for soils with known copper contamination.

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Assessment of Damage to Human Platelets after Aggregation and Other Injuries by Microscopic Observation and Estimation of Serotonin Uptake

Thrombosis and Haemostasis ◽

10.1055/s-0038-1654139 ◽

1970 ◽

Vol 23 (02) ◽

pp. 261-275 ◽

Cited By ~ 3

Author(s):

G Zbinden ◽

J. N Mehrishi ◽

S Tomlin

Keyword(s):

Platelet Aggregation ◽

Electrophoretic Mobility ◽

Human Platelets ◽

Freezing And Thawing ◽

Low Degree ◽

Microscopic Evaluation ◽

Negative Surface ◽

Negative Surface Charge ◽

Charged Macromolecules ◽

5 Ht Uptake

SummaryThe severity of platelet damage induced by hyper- and hypotonic NaCl solutions and freezing and thawing was assessed by microscopic evaluation and measuring inhibition of 5-HT uptake. The same techniques were used to quantitate the effects of aggregating agents. The positively charged macromolecules PS, Poly-L und Poly-O reduced the net negative surface charge as determined by microelectrophoresis, caused platelet aggregation and inhibited 5-HT uptake. The damaging effects of Poly-L and Poly-O were more severe and more closely related to concentration than that of PS. The negatively charged macromolecules Poly-IC and NaPS increased the anodic electrophoretic mobility. Poly-IC and heparin caused a low degree of platelet clumping and no inhibition of 5-HT uptake. NaPS produced severe platelet damage with extensive clumping and complete inhibition of 5-HT uptake. Na laurate had the same effect, but did not alter electrophoretic mobility. ADP caused concentration-dependent platelet aggregation and inhibition of 5-HT uptake. The effects of ADP and NaPS were compared in agitated and non-agitated platelet samples containing identical concentrations of the 2 compounds. Agitation was found to increase the degree of platelet clumping and to reduce 5-HT uptake.

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