Mutagenic effects of mercury pollution as revealed by micronucleus test on three Amazonian fish species

Vigabatrin (VGB) is an antiepileptic drug thatincreases brain γ-aminobutyric acid (GABA) levels through irreversible inhibition of GABA transaminase. The aim of this study was to evaluate neurotoxicological effects of VGB measuring motor activity and genotoxic and mutagenic effects after a single and repeated administration. Male Wistar rats received saline, VGB 50, 100, or 250 mg/kg by gavage for acute and subchronic (14 days) treatments and evaluated in the rotarod task. Genotoxicity was evaluated using the alkaline version of the comet assay in samples of blood, liver, hippocampus, and brain cortex after both treatments. Mutagenicity was evaluated using the micronucleus test in bone marrow of the same animals that received subchronic treatment. The groups treated with VGB showed similar performance in rotarod compared with the saline group. Regarding the acute treatment, it was observed that only higher VGB doses induced DNA damage in blood and hippocampus. After the subchronic treatment, VGB did not show genotoxic or mutagenic effects. In brief, VGB did not impair motor activities in rats after acute and subchronic treatments. It showed a repairable genotoxic potential in the central nervous system since genotoxicity was observed in the acute treatment group.

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Mutagenic effects of combinations of chemical carcinogens and environmental pollutants in mice as shown by the micronucleus test

Mutation Research/Environmental Mutagenesis and Related Subjects ◽

10.1016/0165-1161(82)90018-8 ◽

1982 ◽

Vol 97 (1) ◽

pp. 43-48 ◽

Cited By ~ 37

Author(s):

Masao Watanabe ◽

Sachiko Honda ◽

Mikiko Hayashi ◽

Takeshi Matsuda

Keyword(s):

Micronucleus Test ◽

Environmental Pollutants ◽

Chemical Carcinogens ◽

Mutagenic Effects

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Antitumor Organometallics. IV. The Mutagenic Potential of Some Diphenylantimony(III) Dithiophosphorus Derivatives

Metal-Based Drugs ◽

10.1155/mbd.1994.291a ◽

1994 ◽

Vol 1 (4) ◽

pp. 291-297 ◽

Cited By ~ 21

Author(s):

Carmen Socaciu ◽

Ioan Pasca ◽

Cristian Silvestru ◽

Adela Bara ◽

Ionel Haiduc

Keyword(s):

Cytogenetic Analysis ◽

Micronucleus Test ◽

Ascites Tumor ◽

Short Term ◽

Mutagenic Potential ◽

Tumor Bearing ◽

Ehrlich Ascites ◽

Antitumor Properties ◽

Mutagenic Effects ◽

Derivatives Of

Two diphenylantimony(III) derivatives of dithiophosphorus ligands, i.e. Ph2SbS2PPh2 and Ph2SbS2P(OPr-i)2, which were previously found to exhibit antitumor properties, have been now investigated for potential mutagenic effects in healthy and Ehrlich ascites tumor-bearing mice. Two short-term tests, i.e. the micronucleus test and the cytogenetic analysis, were used as end-points for mutagenicity. The results are consistent with a mutagenic potential for both organoantimony(III) compounds tested, the effect being higher for the phosphorodithioato derivative.

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Assessment of the mutagenicity of the technical product of N-(1-ethylpropyl)-2,6-dinitro -3,4-xylidinen

Hygiene and Sanitation ◽

10.47470/0016-9900-2020-99-4-418-424 ◽

2020 ◽

Vol 99 (4) ◽

pp. 418-424

Author(s):

О. В. Егорова ◽

Наталия Алексеевна Илюшина ◽

Н. С. Аверьянова ◽

Г. В. Масальцев ◽

О. О. Дмитричева

Keyword(s):

Bone Marrow ◽

Micronucleus Test ◽

Fold Increase ◽

Negative Control ◽

Mouse Bone Marrow ◽

Technical Product ◽

Mutagenic Property ◽

Mutagenic Effects ◽

Technical Products

Introduction. Evaluation of genotoxicity of the pesticide technical products is one of the mandatory requirements for their toxicological and hygienic assessment. The data about mutagenic property is ambiguous for some pesticides. This may be due to the use of various active ingredients of technical products of the pesticide for testing, as they may have different profiles of relevant impurities, some of which may be potentially genotoxic. Material and methods. A technical product of N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine was tested using the bacterial reverse mutation method with Salmonella typhimurium (Ames test) and the in vivo mammalian micronucleus analysis in mouse bone marrow erythrocytes. Results. Statistically significant dose-dependent mutagenic effects of the technical product of N-(1-ethylpropyl)-2,6-dinitro-3,4-xylidine were revealed for TA97 (+S9 / -S9); TA100 (+S9 / -S9); TA102 (+S9 / -S9) and TA98 (+S9 / -S9) strains. In all cases, the fold increase of the revertant numbers mediated by the tested substance compared with the concurrent negative control was > 2 except TA98 in the presence of S9. In the micronucleus test, the technical product did not induce a statistically significant increase in the frequency of the micronucleated polychromatophilic erythrocytes in CD-1 mouse bone marrow up to 2000 mg/kg bw. Conclusion. The data suggest all technical products of pesticides entering the market should be tested for the potential genotoxicity. In such a case it is necessary to use at least two methods on different test systems for obtaining reliable results.

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Micronucleus frequencies in Astyanax bimaculatus (Characidae) treated with cyclophosphamide or vinblastine sulfate

Genetics and Molecular Biology ◽

10.1590/s1415-47572000000200041 ◽

2000 ◽

Vol 23 (2) ◽

pp. 489-492 ◽

Cited By ~ 29

Author(s):

F.E. Matsumoto ◽

I.M.S. Cólus

Keyword(s):

Body Weight ◽

Fish Species ◽

Micronucleus Test ◽

Positive Control ◽

Native Fish ◽

Toxicity Studies ◽

Vinblastine Sulfate ◽

Positive Controls

Two known mutagenic drugs, cyclophosphamide and vinblastine sulfate, were tested using the micronucleus test in the native fish species, Astyanax bimaculatus, in order to determine which of these drugs and the doses which would be the most adequate for use as positive controls in this species. This Brazilian fish species was chosen because few toxicity studies have used native fish species and this particular species is widely consumed in various regions of Brazil. Three thousand erythrocytes per specimen were scored. Doses of 16 and 8 mg/kg body weight of cyclophosphamide and vinblastine sulfate, respectively, were the most effective in causing micronuclei. Cyclophosphamide was the most mutagenic of the two drugs and is recommended for use as a positive control in A. bimaculatus.

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