Copper inducing effect on laccase production of white rot fungi native from Misiones (Argentina)

2010 ◽  
Vol 46 (6) ◽  
pp. 534-539 ◽  
Author(s):  
María I. Fonseca ◽  
Ernesto Shimizu ◽  
Pedro D. Zapata ◽  
Laura L. Villalba
Keyword(s):  
White Rot Fungi ◽  
White Rot ◽  
Laccase Production

scholarly journals A Novel Approach in Crude Enzyme Laccase Production and Application in Emerging Contaminant Bioremediation

Processes ◽  
2020 ◽  
Vol 8 (6) ◽  
pp. 648
Author(s):  
Luong N. Nguyen ◽  
Minh T. Vu ◽  
Md Abu Hasan Johir ◽  
Nirenkumar Pathak ◽  
Jakub Zdarta ◽  
...  
Keyword(s):  
Steroid Hormones ◽  
Liquid Culture ◽  
Emerging Contaminants ◽  
White Rot Fungi ◽  
Enzyme Concentration ◽  
Crude Enzyme ◽  
White Rot ◽  
Laccase Production ◽  
Solid Culture ◽  
Novel Approach

Laccase enzyme from white-rot fungi is a potential biocatalyst for the oxidation of emerging contaminants (ECs), such as pesticides, pharmaceuticals and steroid hormones. This study aims to develop a three-step platform to treat ECs: (i) enzyme production, (ii) enzyme concentration and (iii) enzyme application. In the first step, solid culture and liquid culture were compared. The solid culture produced significantly more laccase than the liquid culture (447 vs. 74 µM/min after eight days), demonstrating that white rot fungi thrived on a solid medium. In the second step, the enzyme was concentrated 6.6 times using an ultrafiltration (UF) process, resulting in laccase activity of 2980 µM/min. No enzymatic loss due to filtration and membrane adsorption was observed, suggesting the feasibility of the UF membrane for enzyme concentration. In the third step, concentrated crude enzyme was applied in an enzymatic membrane reactor (EMR) to remove a diverse set of ECs (31 compounds in six groups). The EMR effectively removed of steroid hormones, phytoestrogen, ultraviolet (UV) filters and industrial chemical (above 90%). However, it had low removal of pesticides and pharmaceuticals.

scholarly journals Enhanced Production of Ligninolytic Enzymes by a Mushroom Stereum ostrea

2014 ◽  
Vol 2014 ◽  
pp. 1-9 ◽  
Author(s):  
K. Y. Usha ◽  
K. Praveen ◽  
B. Rajasekhar Reddy
Keyword(s):  
Solid State ◽  
Copper Sulphate ◽  
White Rot Fungi ◽  
Ligninolytic Enzymes ◽  
Tween 80 ◽  
Veratryl Alcohol ◽  
White Rot ◽  
Tween 20 ◽  
Laccase Production ◽  
Enhanced Production

The white rot fungi Stereum ostrea displayed a wide diversity in their response to supplemented inducers, surfactants, and copper sulphate in solid state fermentation. Among the inducers tested, 0.02% veratryl alcohol increased the ligninolytic enzyme production to a significant extent. The addition of copper sulphate at 300 μM concentration has a positive effect on laccase production increasing its activity by 2 times compared to control. Among the surfactants, Tween 20, Tween 80, and Triton X 100, tested in the studies, Tween 80 stimulated the production of ligninolytic enzymes. Biosorption of dyes was carried out by using two lignocellulosic wastes, rice bran and wheat bran, in 50 ppm of remazol brilliant blue and remazol brilliant violet 5R dyes. These dye adsorbed lignocelluloses were then utilized for the production of ligninolytic enzymes in solid state mode. The two dye adsorbed lignocelluloses enhanced the production of laccase and manganese peroxidase but not lignin peroxidase.

scholarly journals Comparative study on laccase activity of white rot fungi under submerged fermentation with different lignocellulosic wastes

BioResources ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. 9166-9179
Author(s):  
Qi An ◽  
Jie Qiao ◽  
Lu-Sen Bian ◽  
Mei-Ling Han ◽  
Xun-You Yan ◽  
...  
Keyword(s):  
Submerged Fermentation ◽  
Laccase Activity ◽  
White Rot Fungi ◽  
Industrial Applications ◽  
Culture Conditions ◽  
White Rot ◽  
Laccase Production ◽  
Poplar Wood ◽  
Lignocellulosic Wastes ◽  
Cottonseed Hull

Different Pleurotus ostreatus and Flammulina velutipes species were compared relative to their ability to produce laccase in submerged fermentation of various lignocellulosic wastes. Fungi cultivation in identical culture conditions revealed wide differences among both species and strains of the same species. The laccase secretion ability of P. ostreatus strains was superior to F. velutipes strains. Maximum laccase production on cottonseed hull, corncob, and poplar wood was secreted by P. ostreatus CY 568, P. ostreatus CCEF 89, and P. ostreatus CY 568, respectively. The nature of lignocellulosic materials played an important role in determining the expression of laccase potential of fungi. The presence of cottonseed hull improved laccase activity and accelerated the rate of enzyme production. Maximum laccase production on cottonseed hull was nearly 1.29-fold and 1.53-fold higher than that on corncob and poplar wood, respectively. Laccase activity was detected in almost all tested strains on cottonseed hull on the first day, while only a few strains on poplar wood and corncob were detected on the first day. These findings will be helpful for selecting the appropriate strain in industrial applications and for optimization of integrated industrial laccase production.

Bioremediation of a wine distillery wastewater using white rot fungi and the subsequent production of laccase

2007 ◽  
Vol 56 (2) ◽  
pp. 179-186 ◽  
Author(s):  
P.J. Strong ◽  
J.E. Burgess
Keyword(s):  
Biological Treatment ◽  
Oxygen Demand ◽  
White Rot Fungi ◽  
White Rot ◽  
White Rot Fungus ◽  
Laccase Production ◽  
Total Phenolic ◽  
Pycnoporus Cinnabarinus ◽  
Distillery Wastewater

The aim of this work was to ascertain whether a submerged culture of a white rot fungus could be used to treat distillery wastewater, and whether the compounds present in the wastewater would stimulate laccase production. Trametes pubescens MB 89, Ceriporiopsissubvermispora, Pycnoporus cinnabarinus and UD4 were screened for their ability for the bioremediation of a raw, untreated distillery wastewater as well as distillery wastewater that had been pretreated by polyvinylpolypyrrolidone. Suitability of each strain was measured as a function of decreasing the chemical oxygen demand (COD) and total phenolic compounds concentration and the colour of the wastewater, while simultaneously producing laccase in high titres. After screening, T. pubescens MB 89 was used further in flask cultures and attained 79±1.1% COD removal, 80±4.6% total phenols removal, 71±1.6% decrease in colour at an absorbance of 500 nm and increased the pH from 5.3 to near-neutral. Laccase activity in flask cultures peaked at 4,644±228 units/l, while the activity in a 50 l bubble lift reactor peaked at 12,966±71 units/l. Trametes pubescens MB 89 greatly improved the quality of a wastewater known for toxicity towards biological treatment systems, while simultaneously producing an industrially relevant enzyme.

scholarly journals Evaluation of laccase production by two white-rot fungi using solid-state fermentation with different agricultural and forestry residues

BioResources ◽  
2021 ◽  
Vol 16 (3) ◽  
pp. 5287-5300
Author(s):  
Qi An ◽  
Cong-Sheng Li ◽  
Jing Yang ◽  
Si-Yu Chen ◽  
Kai-Yue Ma ◽  
...  
Keyword(s):  
Solid State ◽  
Solid State Fermentation ◽  
Low Cost ◽  
White Rot Fungi ◽  
White Rot ◽  
Laccase Production ◽  
Sophora Japonica ◽  
Significant Difference ◽  
Cottonseed Hull ◽  
Forestry Residues

Pleurotus ostreatus and a newly isolated Ganoderma lingzhi strain were evaluated for their laccase secretion capacity by solid-state fermentation with different agricultural and forestry residues. There was a significant difference among fungi for biosynthetic potential. In principle, the laccase secretion capacity of P. ostreatus CY 568 was stronger than that from G. lingzhi Han 500. Different species of fungi had a preference for agricultural and forestry residues. The presence of cottonseed hull and Populus beijingensis were helpful for accelerating the rate of laccase enzyme production of P. ostreatus CY 568. Cottonseed hull and corncob were useful for improving the production of laccase from G. lingzhi Han 500. Continuous and stable laccase production was found on cottonseed hull by P. ostreatus CY 568 and G. lingzhi Han 500. Maximum laccase activity obtained from P. ostreatus CY 568 on Toona sinensis, Sophora japonica, Salix babylonica, Populus beijingensis, corncob, cottonseed hull, and straw of Oryza sativa was higher than that from G. lingzhi Han 500, and was nearly 1.16-fold, 1.59-fold, 3.32-fold, 1.39-fold, 1.08-fold, 1.08-fold, and 1.36-fold, respectively. These findings will be helpful for developing new productive strains and expanding more species for industrial application to obtain efficient and low-cost laccase.

Optimized production, purification and molecular characterization of fungal laccase through Alternaria alternata

2018 ◽  
Vol 43 (6) ◽  
pp. 613-622 ◽  
Author(s):  
Muhammad Irfan ◽  
Sajid Mehmood ◽  
Muhammad Irshad ◽  
Zahid Anwar
Keyword(s):  
Molecular Weight ◽  
Alternaria Alternata ◽  
Polyacrylamide Gel Electrophoresis ◽  
Sodium Dodecyl ◽  
Research Work ◽  
White Rot Fungi ◽  
Industrial Applications ◽  
White Rot ◽  
Laccase Production ◽  
Sds Page

Abstract Objective Industrial effluents and agriculture biomass are main environmental hazards which are facing by developing country like Pakistan. Along with various other industrial applications, laccases are also involved in the oxidation of various industrial hazardous compounds to detoxify them. This study was designed to produce and purify laccase from ascomyceteous fungi, i.e. Alternaria alternata through solid stat fermentation. Materials and methods Abundantly available Sarkanda grass “Saccharum spontaneum” was used as agro-waste substrate for laccase production from fungus A. alternata. Previously only white rot fungi are familiar for laccase production and almost no work has been done on laccase production by A. alternata. In this research work, different physical and chemical parameters were optimized for maximum laccase production through solid state fermentation (SSF). Results Enzyme was purified and its molecular weight was determined by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Maximum laccase activity (21.87±0.0115 unit/mL) was detected on 7th day of incubation having pH 5 of the medium at 35°C. None of the added metal ions increased laccase production. Galactose and “yeast extract” used as optimum carbon and nitrogen source for highest laccase production. Conclusion A monomeric protein (laccase) having approximately 51 kDa molecular weight obtained after SDS-PAGE.

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