Biosensor analyzer for BOD index express control on the basis of the yeast microorganisms Candida maltosa, Candida blankii, and Debaryomyces hansenii

The occurrence of killer activity against a panel composed of 22 industrially and (or) medically important yeasts was investigated in 438 yeast and yeast-like cultures belonging to 96 species, isolated from different environments of the Brazilian rain forest. Altogether, 26% of ascomycetes, 56% of basidiomycetes, and 42% of yeast-like cultures exhibited killer activity against at least one of the panel yeasts. More than 15 species never reported before as toxin producers were found, with Pseudozyma antarctica, Trichosporon asteroides, and Geotrichum klebahnii, showing the broader activity spectra. Plasmid curing did not cure the killer phenotypes of Candida maltosa, Debaryomyces hansenii, G. klebahnii, Tr. asteroides, Cryptococcus laurentii, and Ps. antarctica.Key words: yeasts, killer activity, tropical environments.

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Large-scale screening of selected Candida maltosa, Debaryomyces hansenii and Pichia anomala killer toxin activity against pathogenic yeasts

Medical Mycology ◽

10.1080/714031067 ◽

2001 ◽

Vol 39 (6) ◽

pp. 479-482 ◽

Cited By ~ 17

Author(s):

P. Buzzini ◽

A. Martini

Keyword(s):

Large Scale ◽

Debaryomyces Hansenii ◽

Killer Toxin ◽

Pichia Anomala ◽

Candida Maltosa ◽

Large Scale Screening

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Characterisation of Lactobacillus rhamnosus VT1 and its effect on the growth of Candida maltosa YP1

Czech Journal of Food Sciences ◽

10.17221/680-cjfs ◽

2008 ◽

Vol 25 (No. 5) ◽

pp. 272-282 ◽

Cited By ~ 2

Author(s):

D. Liptáková ◽

Ľ. Valík ◽

A. Lauková ◽

V. Strompfová

Keyword(s):

Growth Rate ◽

Incubation Temperature ◽

Lactobacillus Rhamnosus ◽

Microbial Interactions ◽

Lag Phase ◽

Regression Equations ◽

Candida Maltosa ◽

Food Protection ◽

Spoilage Yeast ◽

Standard Response

The combined effect of initial amount of 18 h L. rhamnosus VT1 inoculum and incubation temperature on the growth of Candida maltosa YP1, an oxidative food spoilage yeast strain, was primarily modelled and studied by standard response surface methodology. This study resulted in the following linear regression equations characterising lag time and growth rate of C. maltosa YP1 in milk in competition with the potentially protective lactobacillus strain. Lag-phase of C. maltosa was strongly influenced by the amount of lactobacillus inoculum (V0) and incubation temperature (1/T). The synergic effect of both these factors was also evident as results from the equation lag = –33.50 + 186.38 × V0 × 1/T + 512.27 × 1/T – 5.511 × V0 (R2(λ) = 0.849). The growth rate was sufficiently described by the linear relation: GrCm = –0.00046 + 0.0033 × T – 0.0016 × V0 (R2(Gr) = 0.847). On the basis of these equations, the mutual microbial interactions and the potential application of the lactobacillus strains to food protection are discussed.

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Rock Surface Fungi in Deep Continental Biosphere—Exploration of Microbial Community Formation with Subsurface In Situ Biofilm Trap

Microorganisms ◽

10.3390/microorganisms9010064 ◽

2020 ◽

Vol 9 (1) ◽

pp. 64

Author(s):

Maija Nuppunen-Puputti ◽

Riikka Kietäväinen ◽

Lotta Purkamo ◽

Pauliina Rajala ◽

Merja Itävaara ◽

...

Keyword(s):

Significant Proportion ◽

Debaryomyces Hansenii ◽

Fungal Communities ◽

Fungal Colonization ◽

Rock Surface ◽

Mica Schist ◽

Deep Subsurface ◽

Bacterial Phyla ◽

In Situ Conditions

Fungi have an important role in nutrient cycling in most ecosystems on Earth, yet their ecology and functionality in deep continental subsurface remain unknown. Here, we report the first observations of active fungal colonization of mica schist in the deep continental biosphere and the ability of deep subsurface fungi to attach to rock surfaces under in situ conditions in groundwater at 500 and 967 m depth in Precambrian bedrock. We present an in situ subsurface biofilm trap, designed to reveal sessile microbial communities on rock surface in deep continental groundwater, using Outokumpu Deep Drill Hole, in eastern Finland, as a test site. The observed fungal phyla in Outokumpu subsurface were Basidiomycota, Ascomycota, and Mortierellomycota. In addition, significant proportion of the community represented unclassified Fungi. Sessile fungal communities on mica schist surfaces differed from the planktic fungal communities. The main bacterial phyla were Firmicutes, Proteobacteria, and Actinobacteriota. Biofilm formation on rock surfaces is a slow process and our results indicate that fungal and bacterial communities dominate the early surface attachment process, when pristine mineral surfaces are exposed to deep subsurface ecosystems. Various fungi showed statistically significant cross-kingdom correlation with both thiosulfate and sulfate reducing bacteria, e.g., SRB2 with fungi Debaryomyces hansenii.

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Debaryomyces hansenii F39A as biosorbent for textile dye removal

Revista Argentina de Microbiología ◽

10.1016/j.ram.2020.10.004 ◽

2021 ◽

Author(s):

Florencia Ruscasso ◽

Brenda Bezus ◽

Gabriela Garmendia ◽

Silvana Vero ◽

Gustavo Curutchet ◽

...

Keyword(s):

Dye Removal ◽

Debaryomyces Hansenii ◽

Textile Dye

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Residual Brewing Yeast as Substrate for Co-Production of Cell Biomass and Biofilm Using Candida maltosa SM4

Fermentation ◽

10.3390/fermentation7020084 ◽

2021 ◽

Vol 7 (2) ◽

pp. 84

Author(s):

Vidal Flores-Copa ◽

Luis Romero-Soto ◽

Danitza Romero-Calle ◽

María Teresa Alvarez-Aliaga ◽

Felipe Orozco-Gutierrez ◽

...

Keyword(s):

Logistic Model ◽

Water Retention ◽

Candida Maltosa ◽

Brewing Yeast ◽

Cell Biomass ◽

Glycosidic Bonds ◽

Biofilm Production ◽

High Degree ◽

Maximal Production ◽

Different Levels

Candida maltosa was cultivated in the liquid phase of residual brewing yeast, a major brewery residue, to produce biomass and biofilm. Using response surface methodology, the effect of two variables at two different levels was investigated. The independent variables were agitation speed (at 100 and 200 rpm), and aeration (at 1 and 3 L min−1). Aeration was identified to be important for the production of both biomass and biofilm, while agitation was the only factor significantly affecting biofilm production. The maximal production of biofilm (2.33 g L−1) was achieved for agitation of 200 rpm and aeration of 1 L min−1, while the maximum for biomass (16.97 g L−1) was reached for 100 rpm agitation and 3 L min−1 air flow. A logistic model applied to predict the growth of C. maltosa in the exponential phase and the biofilm production, showed a high degree of agreement between the prediction and the actual biomass measured experimentally. The produced biofilms were further characterized using Fourier-transform infrared spectroscopy (FTIR), Scanning Electron Microscopy (SEM) and Thermogravimetric Analysis (TGA). FTIR allowed the identification of methyl, carbonyl ester and sulfate groups, and revealed the presence of uronic acid moieties and glycosidic bonds. Water-retention ability up to relatively high temperatures was revealed by TGA, and that makes the produced biofilm suitable for production of hydrogels. SEM also gave indications on the hydrogel-forming potential of the biofilm.

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