Immunomodulatory activity of Ulmus davidiana Planch (Ulmaceae) water and ethanolic extracts on bone cells: Stimulation of proliferation, alkaline phosphatase activity and type I collagen synthesis

2007 ◽  
Vol 23 (2) ◽  
pp. 154-161 ◽  
Author(s):  
Un-Do Lee ◽  
Seok-Jong Suh ◽  
Kap-Sung Kim ◽  
Dong-Soo Kim ◽  
Un-Ho Jin ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Alkaline Phosphatase Activity ◽  
Collagen Synthesis ◽  
Type I Collagen ◽  
Bone Cells ◽  
Immunomodulatory Activity ◽  
Type I ◽  
Ethanolic Extracts ◽  
Ulmus Davidiana ◽  
Stimulation Of

scholarly journals Cytocompatibility evaluation of hydroxyapatite/collagen composites doped with Zn+2

2007 ◽  
Vol 12 (2) ◽  
pp. 307-312 ◽  
Author(s):  
Maria Helena Santos ◽  
Ana Paula M. Shaimberg ◽  
Patricia Valerio ◽  
Alfredo M. Goes ◽  
Maria de Fátima Leite ◽  
...  
Keyword(s):  
Alkaline Phosphatase ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Orthophosphoric Acid ◽  
Type I Collagen ◽  
Enzymatic Digestion ◽  
Osteoblastic Cells ◽  
Type I ◽  
Post Test ◽  
Synthetic Hydroxyapatite

The cytocompatibility of synthetic hydroxyapatite/collagen composites alone or doped with Zn+2 was tested by using primary culture of osteoblasts. The hydroxyapatite (HAP) was synthesized having calcium hydroxide and orthophosphoric acid as precursors. A new HAP composite was developed adding 1.05 w% of Zn(NO3)2.6H2O forming HAPZn. The pure type I collagen (COL) was obtained from bovine pericardium by enzymatic digestion method. The HAP/COL and HAPZn/COL composites were developed and characterized by SEM/EDS. The cell viability and alkaline phosphatase activity in the presence of composites were evaluated by MTT assay and NBT-BCIP assay, respectively, and compared to osteoblastic cells of the control. Three individual experiments were accomplished in triplicates and submitted to the variance analysis and Bonferroni’s post-test with statistically significant at p<0.05. The HAPZn/COL composite did not stimulate the proliferation and increasing of alkaline phosphatase activity of the osteoblastic cells. The tested composites did not alter the cellular viability neither caused alterations in the cellular morphology in 72 h showing adequate properties for biological applications.

Effects of transforming growth factor-β on normal clonal bone cell populations

1991 ◽  
Vol 69 (2-3) ◽  
pp. 132-140 ◽  
Author(s):  
Rebecca Ber ◽  
Takao Kubota ◽  
Jaro Sodek ◽  
Jane E. Aubin
Keyword(s):  
Alkaline Phosphatase ◽  
Growth Factor ◽  
Phosphatase Activity ◽  
Alkaline Phosphatase Activity ◽  
Transforming Growth Factor ◽  
Bone Cells ◽  
Transforming Growth Factor Β ◽  
Prostaglandin E ◽  
Intracellular Camp ◽  
Type I

Although transforming growth factor-β (TGF-β) has been implicated in the local regulation of bone growth and remodelling, its specific effects on different subpopulations of bone cells have not been elucidated. Cells derived from bone are known to be heterogeneous and include both cells of different lineages and osteoblastic populations with different levels of expression of osteoblast-associated properties. Consequently, we have isolated clonal populations of bone cells to examine more precisely the effects of TGF-β on individual subpopulations. Several clonal populations were isolated by limiting dilution from cells derived from 21-day-old fetal rat calvaria. Two of these clones, RCA 11 and RCB 2, were used here. While the two clones responded similarly to parathyroid hormone (PTH) and isoproterenol (ISP) with increases in intracellular cAMP, prostaglandin E2 (PGE2) elicited a 10-fold higher response in RCB 2 cells compared with RCA 11. RCB 2 cells expressed a 10-fold higher alkaline phosphatase activity compared with RCA 11. Both clones synthesized a variety of bone matrix associated proteins, but only RCA 11 synthesized SPP-1 (osteopontin) constitutively. TGF-β stimulated growth of RCB 2 cells after 24 and 48 h of treatment, but had no effect on growth of RCA 11. TGF-β supported anchorage-independent growth of RCB 2 cells, but not that of RCA 11. A 24-h exposure to TGF-β decreased cAMP responsiveness to PTH and ISP slightly in both clones, but had no effect on PGE2 responses. Significant reductions in alkaline phosphatase activity were seen in both clones after 24- and 48-h treatments with TGF-β. Total protein synthesis as measured by [35S]methionine incorporation was stimulated significantly in both clones, but TGF-β selectively stimulated type I collagen compared with type III collagen. SPARC (osteonectin) and secreted phosphoprotein 1 (SPP-1; osteopontin) were stimulated by TGF-β in both RCA 11 and RCB 2 cells. These results indicate that individual clonal populations of cells within bone may be modulated differentially by TGF-β.Key words: transforming growth factor-β, osteoblasts, clonal cell lines, matrix synthesis.

Melatonin stimulates proliferation and type I collagen synthesis in human bone cells in vitro

1999 ◽  
Vol 27 (2) ◽  
pp. 106-110 ◽  
Author(s):  
Osamu Nakade ◽  
Hiroki Koyama ◽  
Hirohiko Ariji ◽  
Akihiro Yajima ◽  
Tohru Kaku
Keyword(s):  
Collagen Synthesis ◽  
Type I Collagen ◽  
Bone Cells ◽  
Human Bone ◽  
Type I

Effects of Ulmus davidiana Planch on mineralization, BMP-2, ALP, type I collagen and collagenase-1 in bone cells

2006 ◽  
Author(s):  
SUNG-KOO KANG ◽  
Kap-Sung Kim ◽  
You-Seok Byun ◽  
Kyung-Ho Kim ◽  
In-Sun Lee ◽  
...  
Keyword(s):  
Type I Collagen ◽  
Bone Cells ◽  
Type I ◽  
Ulmus Davidiana
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