P.0111 Altered nitric oxide concentration as the potential link between nitric oxide synthase genetic variation and mental illness pathogenesis

2021 ◽  
Vol 53 ◽  
pp. S80-S81
Author(s):  
R. McNeill ◽  
M. Brum ◽  
K. Knopf ◽  
N. Brunkhorst-Kanaan ◽  
S. Etyemez ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Mental Illness ◽  
Genetic Variation ◽  
Nitric Oxide Synthase ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration ◽  
Potential Link ◽  
Oxide Synthase

scholarly journals New Modified Method for Determination of Nitric Oxide Synthase Activity in Plasma of Vitiligo Patients

2018 ◽  
Vol 34 (5) ◽  
pp. 2502-2509
Author(s):  
Jwan Abdulmohsin Zainulabdeen ◽  
Aymen Abdulsattar Al-kinani
Keyword(s):  
Oxidative Stress ◽  
Nitric Oxide ◽  
Nitric Oxide Synthase ◽  
Healthy Individuals ◽  
Modified Method ◽  
Oxide Concentration ◽  
Nitric Oxide Concentration ◽  
And Gender ◽  
Oxide Synthase

Vitiligo is a non-contagious skin disorder that characterized by depigmentation of skin due to melanocyte impairment which may be caused to increase levels of free radicals (such as superoxide and nitric oxide) that causing an increase in oxidative stress. The purpose of this study was measured by the activity of oxide synthase (NOS) by our modified method and nitric oxide concentration in plasma of vitiligo patients. The activity of nitric oxide synthase was determined via a modified method by coupling two methods; the first method was based on converting L-arginine to L-citrulline and nitric oxide and the second was used to measure the concentration of nitric oxide. This modified method was applied to patients with vitiligo disease and healthy individuals who matched in age and gender with patients. The condition of this modified method was optimized and the results revealed the following: the activity of NOS was higher in a solution that contains: Tris buffer (50mM), arginine (100mM), calcium chloride (20mM), and NADPH (5mM) during 30 minutes, meanwhile the precision of this method was 2.03. In the current study, the results show that the levels of NOS activity and nitric oxide were affected by the disease in which both parameters appeared highly significant increases in vitiligo patients (p=0.000 and 0.002 respectively) in comparison with the healthy individuals. Results of the experiments proved that it is possible to depend on the modified method to measure the activity of nitric oxide synthase (NOS). Also, the increased levels of NOS activity and nitric oxide concentration in vitiligo patients support the autocytotoxic hypothesis which suggests that oxidative stress may have a role in melanocyte impairment.

scholarly journals Endothelial Nitric Oxide Synthase Haplotypes Are Associated with Features of Metabolic Syndrome

2007 ◽  
Vol 53 (1) ◽  
pp. 91-97 ◽  
Author(s):  
José L González-Sánchez ◽  
María T Martínez-Larrad ◽  
María E Sáez ◽  
Carina Zabena ◽  
María J Martínez-Calatrava ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Insulin Resistance ◽  
Metabolic Syndrome ◽  
Genetic Variation ◽  
Nitric Oxide Synthase ◽  
Endothelial Nitric Oxide Synthase ◽  
Hdl Cholesterol ◽  
Enos Gene ◽  
Oxide Synthase ◽  
Endothelial Nitric Oxide

Abstract Background: The metabolic syndrome, a cluster of several metabolic disorders, is increasingly being recognized as a risk factor for cardiovascular disease. Endothelium-derived nitric oxide facilitates skeletal muscle glucose uptake, and data from animal models indicate that endothelial nitric oxide synthase (eNOS) gene–null mice present with a phenotype of insulin resistance, hypertension, and hypertriglyceridemia, much like that observed in humans with metabolic syndrome. We used haplotype tagging single nucleotide polymorphisms (htSNPs) to investigate the role of genetic variation in the eNOS gene (NOS3) in metabolic syndrome in humans. Methods: We recruited 738 unrelated persons from a cross-sectional population-based epidemiological survey in the province of Segovia in Central Spain (Castille). Metabolic syndrome was defined according to the recently modified National Cholesterol Education Program Adult Treatment Panel III guidelines. Results: Haplotype analysis showed a statistically significant association between some NOS3 gene variants and features of metabolic syndrome. Relative to the most common haplotype, 121, the haplotype 212 was associated with an increased odds ratio (OR) for metabolic syndrome [OR = 1.81, 95% confidence interval (CI) 1.15–2.84], and for decreased HDL-cholesterol concentrations (OR 1.52, 95% CI 1.01–2.29), and with increased mean values for the homeostasis model assessment of insulin resistance (P = 0.043), and triglycerides (P = 0.026). Conclusions: Our results suggest that genetic variation at the eNOS locus is associated with features of metabolic syndrome, and might represent a new genetic susceptibility component for insulin resistance, hypertriglyceridemia, and low HDL-cholesterol concentrations.

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