Effects of the secretome of human Wharton's jelly mesenchymal stem cells on the proliferation and apoptosis gene expression of the retinal pigmented epithelium

The present work described a bio-functionalized 3D fibrous construct, as an interactive teno-inductive graft model to study tenogenic potential events of human mesenchymal stem cells collected from Wharton’s Jelly (hWJ-MSCs). The 3D-biomimetic and bioresorbable scaffold was functionalized with nanocarriers for the local controlled delivery of a teno-inductive factor, i.e., the human Growth Differentiation factor 5 (hGDF-5). Significant results in terms of gene expression were obtained. Namely, the up-regulation of Scleraxis (350-fold, p ≤ 0.05), type I Collagen (8-fold), Decorin (2.5-fold), and Tenascin-C (1.3-fold) was detected at day 14; on the other hand, when hGDF-5 was supplemented in the external medium only (in absence of nanocarriers), a limited effect on gene expression was evident. Teno-inductive environment also induced pro-inflammatory, (IL-6 (1.6-fold), TNF (45-fold, p ≤ 0.001), and IL-12A (1.4-fold)), and anti-inflammatory (IL-10 (120-fold) and TGF-β1 (1.8-fold)) cytokine expression upregulation at day 14. The presented 3D construct opens perspectives for the study of drug controlled delivery devices to promote teno-regenerative events.

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Evaluation of NLR Family CARD Domain Containing 3 and NLR Family CARD Domain Containing 5 Gene Expression in Interferon Gamma–Treated Mesenchymal Stem Cells from Wharton’s Jelly of Human Umbilical Cord

Gene Cell and Tissue ◽

10.5812/gct.118882 ◽

2021 ◽

Vol In Press (In Press) ◽

Author(s):

Aida Nahumi ◽

Leila Pirdel ◽

Asadollah Asadi ◽

Arash Abdolmaleki

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Interferon Gamma ◽

Wharton’S Jelly ◽

Human Umbilical Cord ◽

Wharton's Jelly ◽

Card Domain ◽

Ifn Γ

Background: Human umbilical cord Wharton’s jelly has provided a new source for mesenchymal stem cells (MSCs). The highly proliferative capacity with low immunogenicity and multi-differentiation potential of its stem cells make them applicable for transplantation purposes. Nucleotide-binding oligomerization domain (NOD)-like receptors (NLRs) play various roles in antigen presentation of pathogens and damaged cells to suppress and/or modulate inflammation. Objectives: In this study, the expression levels of NLR family CARD domain containing 3 (NLRC3) and NLRC5 genes were analyzed and compared in both untreated and interferon gamma (IFN-γ)–treated Wharton’s jelly-derived MSCs (WJ-MSCs). Methods: MSCs were isolated from human umbilical cord Wharton’s jelly using standard tissue culture. The expression of NLRC5 and NLRC3 genes was analyzed in IFN-γ–treated WJ-MSCs (24 hours after treatment) and untreated WJ-MSCs (as a control) using quantitative real-time polymerase chain reaction (PCR). Results: It was found that IFN-γ treatment mimicking an inflammation scenario led to a statistically significant increase of NLRC3 and NLRC5 gene expression compared to untreated WJ-MSCs (P ≤ 0.05). Conclusions: It seems that higher expression of NLRC3 and NLRC5 genes in treated WJ-MSCs may make them a proper candidate to be used as a source for cell therapy in inflammatory conditions.

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Differential Expression of Osteogenic Differentiation in Human Umbilical Cord Wharton’s Jelly-Derived Mesenchymal Stem Cells Treated with Demineralized Bone

Advanced Materials Research ◽

10.4028/www.scientific.net/amr.55-57.697 ◽

2008 ◽

Vol 55-57 ◽

pp. 697-700 ◽

Cited By ~ 2

Author(s):

D. Dhitiseith ◽

S. Honsawek

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Wharton’S Jelly ◽

Human Umbilical Cord ◽

Activity Assay ◽

Rt Pcr ◽

Wharton's Jelly ◽

Osteogenic Lineage

Mesenchymal stem cells are multipotential cells capable of differentiating into osteoblasts, chondrocytes, adipocytes, tenocytes, and myoblasts. Wharton’s jelly consists of stem cells that are a rich source of primitive multipotent mesenchymal cells. Demineralized bone matrix (DBM) has been widely utilized as a biomaterial to promote new bone formation. We isolate and characterize umbilical cord Wharton’s Jelly-derived mesenchymal stem (UCMS) cells derived from Wharton’s jelly and examine the biological activity of DBM in this cell line. Osteoblast differentiation of the UCMS cells was determined using alkaline phosphatase (ALP) activity assay. To examine differential gene expression during osteogenic differentiation, total RNA was isolated from UCMS cells in the absence or presence of DBM on day7 and analyzed using osteogenesis cDNA gene array. The selected genes were verified using reverse transcriptase-polymerase chain reaction (RT-PCR) analyses. Wharton’s jelly derived cells could differentiate along an osteogenic lineage after treatment of DBM. The ALP activity assay showed that human UCMS cells could differentiate into osteogenic lineage. Gene expression of human UCMS cells treated with DBM for 7 days was analyzed by using cDNA array and RT-PCR analyses. We found that expression of RUNX2 and SMAD2 was upregulated whereas SMAD7 expression was downregulated as confirmed by RT-PCR. UCMS cells from a Wharton’s jelly of human umbilical cord could express osteogenesis genes for treatment with DBM. Wharton’s jelly from umbilical cord is a new source of mesenchymal stem cells that are readily available for application to bone tissue engineering.

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Influence of the Type of Delivery, Use of Oxytocin, and Maternal Age on POU5F1 Gene Expression in Stem Cells Derived from Wharton’s Jelly within the Umbilical Cord

Oxidative Medicine and Cellular Longevity ◽

10.1155/2019/1027106 ◽

2019 ◽

Vol 2019 ◽

pp. 1-8 ◽

Cited By ~ 1

Author(s):

Paulina Gil-Kulik ◽

Piotr Chomik ◽

Arkadiusz Krzyżanowski ◽

Elżbieta Radzikowska-Büchner ◽

Ryszard Maciejewski ◽

...

Keyword(s):

Gene Expression ◽

Stem Cells ◽

Mesenchymal Stem Cells ◽

Umbilical Cord ◽

Maternal Age ◽

Subsequent Pregnancy ◽

Wharton’S Jelly ◽

Proliferative Potential ◽

Younger Women ◽

Wharton's Jelly

The paper presents an evaluation of the POU5F1 gene expression in mesenchymal stem cells derived from Wharton’s jelly within the umbilical cord, collected from 36 patients during labor. The study is the first one to show that the expression of POU5F1 in mesenchymal stem cells has been dependent on maternal age, birth order, route of delivery, and use of oxytocin. Our research proves that the POU5F1 gene expression in mesenchymal stem cells decreases with each subsequent pregnancy and delivery. Wharton’s jelly stem cells obtained from younger women and during their first delivery, as well as patients treated with oxytocin, show higher POU5F1 gene expression when compared with the subsequent deliveries. This leads to a conclusion that they are characterized by a lower level of differentiation, which in turn results in their greater plasticity and greater proliferative potential. Probably, they are also clinically more useful.

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