scholarly journals Characterisation of corneas following different time and storage methods for their use as a source of stem-like limbal epithelial cells

2021 ◽  
pp. 108720
Author(s):  
Cristina Romo-Valera ◽  
Miguel Pérez-Garrastachu ◽  
Raquel Hernáez-Moya ◽  
Maddalen Rodriguez-Astigarraga ◽  
Paula Romano-Ruiz ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Storage Methods ◽  
And Storage

Comparison of capture and storage methods for aqueous macrobial eDNA using an optimized extraction protocol: advantage of enclosed filter

2016 ◽  
Vol 8 (5) ◽  
pp. 635-645 ◽  
Author(s):  
Johan Spens ◽  
Alice R. Evans ◽  
David Halfmaerten ◽  
Steen W. Knudsen ◽  
Mita E. Sengupta ◽  
...  
Keyword(s):  
Extraction Protocol ◽  
Storage Methods ◽  
And Storage

scholarly journals The expression and localization of V-ATPase and cytokeratin 5 during postnatal development of the pig epididymis

2020 ◽  
Vol 33 (7) ◽  
pp. 1077-1086 ◽  
Author(s):  
Yun-Jae Park ◽  
Ji-Hyuk Kim ◽  
Hack-Youn Kim ◽  
Hee-Bok Park ◽  
Juhui Choe ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Expression Patterns ◽  
Basal Cells ◽  
Cell Type ◽  
Clear Cells ◽  
Specific Distribution ◽  
Cytokeratin 5 ◽  
Cell Type Specific ◽  
Immunofluorescence Labeling ◽  
And Storage

Objective: We examined the localization and expression of H<sup>+ pumping vacuolar ATPase (V-ATPase) and cytokeratin 5 (KRT5) in the epididymis of pigs, expressed in clear and basal cells, respectively, during postnatal development.Methods: Epididymides were obtained from pigs at 1, 7, 21, 60, 120, and 180 days of age; we observed the localization and expression patterns of V-ATPase and KRT5 in the different regions of these organs, namely, the caput, corpus, and cauda. The differentiation of epididymal epithelial cells was determined by immunofluorescence labeling using cell-type-specific markers and observed using confocal microscopy.Results: At postnatal day 5 (PND5), the localization of clear cells commenced migration from the cauda toward the caput. Although at PND120, goblet-shaped clear cells were detected along the entire length of the epididymis, those labeled for V-ATPase had disappeared from the corpus to cauda and were maintained only in the caput epididymis in adult pigs. In contrast, whereas basal cells labeled for KRT5 were only present in the vas deferens at birth, they were detected in all regions of the epididymis at PND60. These cells were localized at the base of the epithelium; however, no basal cells characterized by luminally extending cell projections were observed in any of the adult epididymides examined.Conclusion: The differentiation of clear and basal cells progressively initiates in a retrograde manner from the cauda to the caput epididymis. The cell-type-specific distribution and localization of the epithelial cells play important roles in establishing a unique luminal environment for sperm maturation and storage in the pig epididymis.

scholarly journals Modern concepts of conversion and storage of energy by dispersed materials absorption

2002 ◽  
Vol 34 (3) ◽  
pp. 247-259
Author(s):  
Dragica Minic-Popovic ◽  
M.V. Susic
Keyword(s):  
Research And Development ◽  
Metal Hydride ◽  
Development Stage ◽  
Ease Of Use ◽  
Glass Microsphere ◽  
Carbon Clusters ◽  
Storage Method ◽  
Advantages And Disadvantages ◽  
Storage Methods ◽  
And Storage

Once hydrogen is generated, the question asked: How do we store hydrogen? Hydrogen can be stored in a variety of ways, each with specific advantages and disadvantages. The overall criteria for choosing a storage method should be safety and ease of use. Described in this paper and listed below are different storage methods available today (compressed hydrogen, liquid carrier storage, glass microsphere, chemically stored hydrogen) in addition to some techniques that are still in the research and development stage: power balls, metal hydride tanks and carbon clusters.

scholarly journals EFFECT OF PLANTING TIME AND STORAGE METHODS ON GRAIN WEEVILS, SITOPHILUS SPP (COLEOPTERA: CURCULIONIDAE) INFESTING WHEAT GRAINS

2017 ◽  
Vol 2 (2) ◽  
pp. 101-109
Author(s):  
M.B. Attia ◽  
A.I. Farag ◽  
Saadeia M. Saed ◽  
Nehal O. Swelam
Keyword(s):  
Wheat Grains ◽  
Planting Time ◽  
Storage Methods ◽  
And Storage
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