Human peripheral blood mononuclear cells as an ex vivo model to study the host parasite interaction in Toxoplasma gondii

2020 ◽  
Vol 219 ◽  
pp. 108020
Author(s):  
Alejandro Acosta-Dávila ◽  
Alejandra Acosta-Espinel ◽  
Alejandro Hernández-de-Los-Ríos ◽  
Jorge Enrique Gómez-Marín
Keyword(s):  
Toxoplasma Gondii ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Human Peripheral Blood ◽  
Ex Vivo Model ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells ◽  
Host Parasite

A pathway analysis of poly(I:C)-induced global gene expression change in human peripheral blood mononuclear cells

2006 ◽  
Vol 26 (2) ◽  
pp. 125-133 ◽  
Author(s):  
C. Chris Huang ◽  
Karen E. Duffy ◽  
Lani R. San Mateo ◽  
Bernard Y. Amegadzie ◽  
Robert T. Sarisky ◽  
...  
Keyword(s):  
Gene Expression ◽  
Viral Infection ◽  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Human Peripheral Blood ◽  
Poly I:C ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells

To gain global pathway perspective of ex vivo viral infection models using human peripheral blood mononuclear cells (PBMCs), we conducted expression analysis on PBMCs of healthy donors. RNA samples were collected at 3 and 24 h after PBMCs were challenged with the Toll-like receptor-3 (TLR3) agonist polyinosinic acid-polycytidylic acid [poly(I:C)] and analyzed by internally developed cDNA microarrays and TaqMan PCR. Our results demonstrate that poly(I:C) challenge can elicit certain gene expression changes, similar to acute viral infection. Hierarchical clustering revealed distinct immediate early, early-to-late, and late gene regulation patterns. The early responses were innate immune responses that involve TLR3, the NF-κB-dependent pathway, and the IFN-stimulated pathway, whereas the late responses were mostly cell-mediated immune response that involve activation of cell adhesion, cell mobility, and phagocytosis. Overall, our results expanded the utilities of this ex vivo model, which could be used to screen molecules that can modulate viral stress-induced inflammation, in particular those mediated via TLRs.

In vitro and ex vivo effect of tiaprofenic acid on human peripheral blood mononuclear cells

1992 ◽  
Vol 14 (7) ◽  
pp. 1279-1284
Author(s):  
Wilma Barcellini ◽  
Maria Orietta Borghi ◽  
Cristina Fain ◽  
Nicoletta Del Papa ◽  
Patrizia Favini ◽  
...  
Keyword(s):  
Peripheral Blood Mononuclear Cells ◽  
Peripheral Blood ◽  
Mononuclear Cells ◽  
Ex Vivo ◽  
Human Peripheral Blood ◽  
Tiaprofenic Acid ◽  
Peripheral Blood Mononuclear ◽  
Blood Mononuclear Cells
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