Genotypic consistency in the expression of leaf water potential in rice (Oryza sativa L.)

Twenty days after rice (Oryza sativaL. ‘IR28’) emergence, water was withheld from the stress treatment for 20 days while the control was kept well-watered by furrow irrigation. In the control plots, rice leaf water potential at 0800 hours was –5.5 bars and leaf length 30 cm on the 12th day of stress. In the stress treatment, leaf water potential decreased to –8 bars and leaf length to 20 cm, when no weeds competed with rice. With maximum weed competition, rice leaf water potential decreased to –18 bars and leaf length to 15.5 cm. on the 20th day of stress, dawn (0600 h) leaf water potential of rice in the stress treatment was reduced from –8 bars when no weeds competed with rice to –19 bars when competition was maximum. The weed species had higher water potential values than the rice in both control and water-stress treatments.

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KANDUNGAN AIR PADA SEGMEN DAUN TANAMAN PADI (Oryza sativa L.) LOKAL SULAWESI UTARA SAAT KEKERINGAN YANG DIINDUKSI DENGAN POLIETILEN GLIKOL 8000

JURNAL ILMIAH SAINS ◽

10.35799/jis.18.2.2018.20301 ◽

2018 ◽

Vol 18 (2) ◽

pp. 63

Author(s):

Risa Junita Mereh ◽

Nio Song Ai ◽

Parluhutan Siahaan

Keyword(s):

Oryza Sativa ◽

Water Potential ◽

Water Content ◽

Oryza Sativa L ◽

Leaf Water ◽

Leaf Segment ◽

Leaf Water Content ◽

North Sulawesi ◽

Leaf Segments ◽

Peg 8000

KANDUNGAN AIR PADA SEGMEN DAUN TANAMAN PADI (Oryza sativa L.) LOKAL SULAWESI UTARA SAAT KEKERINGAN YANG DIINDUKSI DENGAN POLIETILEN GLIKOL 8000 ABSTRAKPenelitian ini telah dilakukan untuk mengkaji kandungan air sebagai respon fisiologi pada segmen daun tanaman padi lokal Sulut (varietas Temo, Ombong, Burungan, dan Superwin) terhadap kekeringan yang diinduksi dengan PEG 8000 secara in vitro. Segmen daun padi dipotong-potong 1 cm x 1 cm dan diberi tiga macam perlakuan PEG 8000 (dengan potensial air/PA medium 0; -0,25; dan -0,5 MPa), dan empat waktu pengambilan sampel (0, 4, 8 dan 12 jam) dalam tiga kali ulangan. Hasil penelitian menunjukkan bahwa bahwa faktor varietas, faktor waktu perlakuan, faktor konsentrasi PEG 8000, dan interaksi antara ketiga faktor tersebut tidak menyebabkan perbedaan kandungan air segmen daun yang nyata.Kata kunci: Cekaman kekeringan, PEG 8000, Potensial air, Segmen daun LEAF WATER CONTENT IN LEAF SEGMENTS OF NORTH SULAWESI LOCAL RICE (Oryza sativa L.) UNDER POLYETHYLENE-GLYCOL- 8000-INDUCED DROUGHTED DROUGHTABSTRACTA study was conducted to evaluate leaf water content as one of physiological responses in leaf segments of North Sulawesi local rice (cv. Temo, Ombong, Burungan, and Superwin) under PEG 8000- induced-drought. The rice leaves were cut into 1 cm x 1 cm segments and treated withPEG 8000 solutions (medium water potential or PA of 0; -0.25; and -0.5 MPa),four sampling times (0, 4, 8 and 12 hours) and 3 replicates. The results indicated that factors of varieties, treatment period, concentration of PEG 8000, and interaction among these factors did not result in any differences in water content of rice leaf segments.Keywords: Drought stress, PEG 8000, Water potential, Leaf segment

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