Effect of Cryoprotective Additives-Reduced Glutathione, Acetyl-L-Carnitine on Sperm Membrane Lipid Peroxidation, DNA Integrity and Recovery of Motile Human Sperm

Boars having normal (71.1 ± 1.2%; n = 10) or low (35.12 &plusmn 3.9%; n = 10) sperm motility 24 h after collection were used, and semen was evaluated following storage in Beltsville Thawing Solution (BTS) for 24 h at 18°C. Sperm lipids were extracted and lipid peroxidation quantified. No differences were evident in fresh semen, but after 24 h, sperm motility, viability and membrane permeability in the low motility group were lower (P < 0.001) compared with the normal motility group. Sperm membrane lipid peroxidation was greater (P < 0.001) in the low motility group. A factor influencing sperm storability is membrane lipid peroxidation, which can be accurately assayed using a commercial kit.Key words: Boars, sperm motility, sperm quality, lipid peroxidation

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Influence of ejaculatory abstinence on seminal total antioxidant capacity and sperm membrane lipid peroxidation

Fertility and Sterility ◽

10.1016/j.fertnstert.2014.05.039 ◽

2014 ◽

Vol 102 (3) ◽

pp. 705-710 ◽

Cited By ~ 29

Author(s):

Paul B. Marshburn ◽

Allie Giddings ◽

Stephanie Causby ◽

Michelle L. Matthews ◽

Rebecca S. Usadi ◽

...

Keyword(s):

Lipid Peroxidation ◽

Antioxidant Capacity ◽

Total Antioxidant Capacity ◽

Membrane Lipid ◽

Membrane Lipid Peroxidation ◽

Total Antioxidant ◽

Sperm Membrane ◽

Ejaculatory Abstinence

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Increased erythrocyte antioxidant status protects against smoking induced hemolysis in moderate smokers

Human & Experimental Toxicology ◽

10.1177/0960327110396527 ◽

2011 ◽

Vol 30 (10) ◽

pp. 1475-1481 ◽

Cited By ~ 20

Author(s):

Padmavathi Pannuru ◽

Damodara Reddy Vaddi ◽

Rameswara Reddy Kindinti ◽

Nallanchakravarthula Varadacharyulu

Keyword(s):

Lipid Peroxidation ◽

Erythrocyte Membrane ◽

Antioxidant Status ◽

Reduced Glutathione ◽

Membrane Lipid ◽

Red Cell ◽

Membrane Lipid Peroxidation ◽

Free Radical Damage ◽

Human Red Blood Cells ◽

Nitrite Nitrate

Cigarette smoking is common in societies worldwide and has been identified as injurious to human health. Human red blood cells are important targets for electrophilic and oxidant foreign compounds. In the present study, the possible role of antioxidant status on smoking-induced erythrocyte hemolysis of smokers was studied. Erythrocyte superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx) activities, reduced glutathione (GSH) level, erythrocyte membrane lipid peroxidation, total cholesterol and phospholipids were determined. Further, nitrite/nitrate levels (NO2/NO3) in both plasma and erythrocyte lysate were measured. Results showed increased plasma and erythrocyte membrane lipid peroxidation and nitrite/nitrate levels in smokers. The activities of SOD, CAT and GPx were also increased with reduced glutathione (GSH) level in smokers. No significant change was observed in smokers red cell hemolysis and cholesterol/phospholipid (C/P) ratio compared to controls. Erythrocyte membrane lipid peroxidation was positively correlated with SOD ( r = 0.482, p < 0.01) and GPx ( r = 0.368, p < 0.018) in smokers. Increased levels of nitrite/nitrate and antioxidant status of erythrocytes might be playing a crucial role in protecting red cell from free radical damage induced by cigarette smoke.

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Mechanistic Insight into the Regulation of Lipoxygenase-Driven Lipid Peroxidation Events in Human Spermatozoa and Their Impact on Male Fertility

Antioxidants ◽

10.3390/antiox10010043 ◽

2020 ◽

Vol 10 (1) ◽

pp. 43

Author(s):

Jessica L. H. Walters ◽

Amanda L. Anderson ◽

Sarah J. Martins da Silva ◽

R. John Aitken ◽

Geoffry N. De Iuliis ◽

...

Keyword(s):

Lipid Peroxidation ◽

Male Infertility ◽

Human Sperm ◽

Protective Role ◽

Membrane Lipid ◽

Human Spermatozoa ◽

Reactive Carbonyl Species ◽

Sperm Membrane ◽

Infertility Patients ◽

Cellular Dysfunction

A prevalent cause of sperm dysfunction in male infertility patients is the overproduction of reactive oxygen species, an attendant increase in lipid peroxidation and the production of cytotoxic reactive carbonyl species such as 4-hydroxynonenal. Our previous studies have implicated arachidonate 15-lipoxygenase (ALOX15) in the production of 4-hydroxynonenal in developing germ cells. Here, we have aimed to develop a further mechanistic understanding of the lipoxygenase-lipid peroxidation pathway in human spermatozoa. Through pharmacological inhibition studies, we identified a protective role for phospholipase enzymes in the liberation of peroxidised polyunsaturated fatty acids from the human sperm membrane. Our results also revealed that arachidonic acid, linoleic acid and docosahexanoic acid are key polyunsaturated fatty acid substrates for ALOX15. Upon examination of ALOX15 in the spermatozoa of infertile patients compared to their normozoospermic counterparts, we observed significantly elevated levels of ALOX15 protein abundance in the infertile population and an increase in 4-hydroxynonenal adducts. Collectively, these data confirm the involvement of ALOX15 in the oxidative stress cascade of human spermatozoa and support the notion that increased ALOX15 abundance in sperm cells may accentuate membrane lipid peroxidation and cellular dysfunction, ultimately contributing to male infertility.

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