Survival rate of human oocytes and pregnancy outcome after vitrification using slush nitrogen in assisted reproductive technologies

Implantation failure and disorders of endometrial receptivity represent an essential cause of infertility; multiple parameters were needed to predict the uterine receptivity understanding that no sole parameter could predict the same. A score was termed as (Uterine Biophysical Profile) could be utilized as a predictor of endometrial receptivity. To evaluate the predictive potential of Uterine biophysical profile of both endometrial receptivity and pregnancy outcome in infertile women undergoing Intrauterine Insemination (IUI). The current cross-sectional study was conducted in the High Institute for Infertility Diagnosis and Assisted Reproductive Technologies in Al Nahrain University, Baghdad, Iraq from the 1st of Oct. 2018 till 1st of May 2019 involving seventy women of infertile couples with the same inclusion and exclusion criteria. Uterine biophysical profile was evaluated using a doppler ultrasound examination and then a score was calculated and correlated to pregnancy outcome. The mean Uterine Artery Pulsatility Index (UAPI) was significantly lower in women with positive pregnancy in comparison to women with negative pregnancy, 2.10±0.19 versus 2.47±0.65, respectively (P=0.032). Moreover, no women with Pulsatility Index (PI) score (0) succeeded to get pregnant and the higher the score, the higher the rate of pregnancy (P=0.006). Furthermore, Spearman correlation showed significant positive correlation between positive pregnancy outcome and UAPI (r=0.365; P=0.002). The mean total score was significantly higher for pregnant women than in women with negative pregnancy, 18.27±1.33 versus 16.35±2.47, respectively (P=0.005). The cutoff value was >17 with an acceptable accuracy level of 74.2. The sensitivity of that cutoff vale was 80 % and the specificity was 65.5%. Uterine artery pulsatility index and total uterine biophysical score are the principal predictors of positive pregnancy outcomes in infertile women undergoing IUI.

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Dynamic regulation of DNA methyltransferases in human oocytes and preimplantation embryos after assisted reproductive technologies

MHR Basic science of reproductive medicine ◽

10.1093/molehr/gau049 ◽

2014 ◽

Vol 20 (9) ◽

pp. 861-874 ◽

Cited By ~ 50

Author(s):

Laetitia Petrussa ◽

Hilde Van de Velde ◽

Martine De Rycke

Keyword(s):

Assisted Reproductive Technologies ◽

Reproductive Technologies ◽

Dna Methyltransferases ◽

Preimplantation Embryos ◽

Dynamic Regulation ◽

Human Oocytes

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Infertility and reproductive disorders: impact of hormonal and inflammatory mechanisms on pregnancy outcome

Human Reproduction Update ◽

10.1093/humupd/dmv044 ◽

2015 ◽

Vol 22 (1) ◽

pp. 104-115 ◽

Cited By ~ 103

Author(s):

Silvia Vannuccini ◽

Vicki L. Clifton ◽

Ian S. Fraser ◽

Hugh S. Taylor ◽

Hilary Critchley ◽

...

Keyword(s):

Pregnancy Outcome ◽

Pregnancy Outcomes ◽

Assisted Reproductive Technologies ◽

Negative Impact ◽

Polycystic Ovary ◽

Reproductive Technologies ◽

Obstetric Complications ◽

Reproductive Disorders ◽

Childbearing Age ◽

Increased Risk

Abstract BACKGROUND Reproductive disorders and infertility are associated with the risk of obstetric complications and have a negative impact on pregnancy outcome. Affected patients often require assisted reproductive technologies (ART) to conceive, and advanced maternal age is a further confounding factor. The challenge is to dissect causation, correlation and confounders in determining how infertility and reproductive disorders individually or together predispose women to poor pregnancy outcomes. METHODS The published literature, to June 2015, was searched using PubMed, summarizing all evidences concerning the perinatal outcome of women with infertility and reproductive disorders and the potential mechanisms that may influence poor pregnancy outcome. RESULTS Reproductive disorders (endometriosis, adenomyosis, polycystic ovary syndrome and uterine fibroids) and unexplained infertility share inflammatory pathways, hormonal aberrations, decidual senescence and vascular abnormalities that may impair pregnancy success through common mechanisms. Either in combination or alone, these disorders results in an increased risk of preterm birth, fetal growth restriction, placental pathologies and hypertensive disorders. Systemic hormonal aberrations, and inflammatory and metabolic factors acting on endometrium, myometrium, cervix and placenta are all associated with an aberrant milieu during implantation and pregnancy, thus contributing to the genesis of obstetric complications. Some of these features have been also described in placentas from ART. CONCLUSIONS Reproductive disorders are common in women of childbearing age and rarely occur in isolation. Inflammatory, endocrine and metabolic mechanisms associated with these disorders are responsible for an increased incidence of obstetric complications. These patients should be recognized as ‘high risk’ for poor pregnancy outcomes and monitored with specialized follow-up. There is a real need for development of evidence-based recommendations about clinical management and specific obstetric care pathways for the introduction of prompt preventative care measures.

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Twin pregnancies after assisted reproductive technologies: the role of maternal age on pregnancy outcome

European Journal of Obstetrics & Gynecology and Reproductive Biology ◽

10.1016/j.ejogrb.2016.09.019 ◽

2016 ◽

Vol 206 ◽

pp. 198-203 ◽

Cited By ~ 6

Author(s):

Serena Pinzauti ◽

Chiara Ferrata ◽

Silvia Vannuccini ◽

Giulia Di Rienzo ◽

Filiberto M. Severi ◽

...

Keyword(s):

Pregnancy Outcome ◽

Maternal Age ◽

Assisted Reproductive Technologies ◽

Reproductive Technologies ◽

Twin Pregnancies

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DYSMORPHISMS OF OOCYTES IN ART PROCEDURES: A LITERATURE REVIEW

Reproductive Medicine ◽

10.37800/rm.3.2021.44-53 ◽

2021 ◽

pp. 44-53

Author(s):

G.M. Karibayeva ◽

S.I. Tevkin ◽

T.M. Jussubaliyeva ◽

M.S. Shishimorova

Keyword(s):

Literature Review ◽

Pregnancy Rate ◽

Assisted Reproductive Technologies ◽

Morphological Changes ◽

Reproductive Technologies ◽

Clinical Pregnancy ◽

Clinical Pregnancy Rate ◽

Human Oocytes

Relevance: Assisted reproductive technologies (ART) are rapidly developing and in recent decades have become increasingly important due to the growing number of infertile couples around the world. Human oocytes are the main objects used in ART procedures. Consequently, the quality of oocytes can determine the key parameters of ART. The purpose of this review was to analyze the literature and the results of studies in the field of ART devoted to extracytoplasmic dysmorphisms of human oocytes – morphological changes outside the cytoplasmic structure of oocytes, their effect on fertilization, cleavage, implantation frequency, clinical pregnancy rate, as well as the possibility of their use as biomarkers for predicting the quality of embryos, blastocysts, and their further implantation potential. Materials and Methods: This literature review was based on a search conducted among domestic and foreign publications for 2000-2020 available in Russian and international search systems (PubMed, eLibrary) using the keywords «infertility,” “IVF,” «oocyte,” “morphological assessment of oocytes,” “dysmorphisms of oocytes ,” and “ assisted reproductive technologies.” Results: This literature review contains literature data and the analysis of research results in the field of ART devoted to the morphological qualities and abnormalities (dysmorphisms) of human oocytes. It describes the types of extracytoplasmic abnormalities encountered in the clinical practice of in-vitro fertilization, their effect on fertilization, cleavage, implantation rate, and clinical pregnancy rate, as well as the possibility of their use as biomarkers to predict the quality of embryos and blastocysts and their further implantation potential.

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Factors VIII and von Willebrand levels in women undergoing Assisted Reproduction: are their levels associated with clinical pregnancy outcome?

Mediterranean Journal of Hematology and Infectious Diseases ◽

10.4084/mjhid.2020.058 ◽

2020 ◽

Vol 12 (1) ◽

pp. e2020058

Author(s):

Monica Attanasio ◽

Michela Cirillo ◽

Maria Elisabetta Coccia ◽

Giancarlo Castaman ◽

Cinzia Fatini

Keyword(s):

Pregnancy Outcome ◽

Assisted Reproductive Technologies ◽

Reproductive Technologies ◽

Oocyte Donation ◽

Clinical Pregnancy ◽

Fertility Treatment ◽

Early Predictors ◽

Biochemical Pregnancy ◽

Coagulation Proteins ◽

Von Willebrand

A growing number of infertile women are considering pregnancy through assisted reproductive technologies; hormonal fertility treatment is associated with a procoagulant milieu. In oocyte donation Assisted Reproductive Technologies there are patients who experience repeated implantation failures, as well as biochemical pregnancy, in particular in women at advanced age (>40 yrs). No information is available concerning coagulation changes in women undergoing oocyte donation. In this study, we decided to identify changes in haemostasis in women undergoing infertility treatment and their relationship with clinical pregnancy outcome. Our findings evidence an early increase of FVIII and VWF coagulation proteins, suggesting their potential role as early “predictors” of a successful clinical pregnancy in oocyte donation women. This may be intriguing for exploring potential mechanisms responsible for the establishment of a successful pregnancy after oocyte donation.

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Re-imagining Reproduction: Unsettling Metaphors in the History of Imperial Science and Commercial Surrogacy in India

Somatechnics ◽

10.3366/soma.2015.0149 ◽

2015 ◽

Vol 5 (1) ◽

pp. 88-103 ◽

Cited By ~ 14

Author(s):

Kalindi Vora

Keyword(s):

Assisted Reproductive Technologies ◽

Reproductive Technologies ◽

The Body ◽

Body Parts ◽

Colonial History ◽

Women's Bodies ◽

Commercial Surrogacy ◽

Art Practices ◽

History Of ◽

Travel Industry

This paper provides an analysis of how cultural notions of the body and kinship conveyed through Western medical technologies and practices in Assisted Reproductive Technologies (ART) bring together India's colonial history and its economic development through outsourcing, globalisation and instrumentalised notions of the reproductive body in transnational commercial surrogacy. Essential to this industry is the concept of the disembodied uterus that has arisen in scientific and medical practice, which allows for the logic of the ‘gestational carrier’ as a functional role in ART practices, and therefore in transnational medical fertility travel to India. Highlighting the instrumentalisation of the uterus as an alienable component of a body and subject – and therefore of women's bodies in surrogacy – helps elucidate some of the material and political stakes that accompany the growth of the fertility travel industry in India, where histories of privilege and difference converge. I conclude that the metaphors we use to structure our understanding of bodies and body parts impact how we imagine appropriate roles for people and their bodies in ways that are still deeply entangled with imperial histories of science, and these histories shape the contemporary disparities found in access to medical and legal protections among participants in transnational surrogacy arrangements.

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