Measuring serum estradiol and progesterone one day prior to frozen embryo transfer improves clinical pregnancy rate

Background The influence of anti-nuclear antibody (ANA) on induced ovulation was controversial, and the effect of prednisone plus hydroxychloroquine (HCQ) treatment on frozen embryo transfer outcomes of in-vitro fertilization (IVF) and intracytoplasmic sperm injection (ICSI) for ANA-positive women was unclear. Methods Fifty ANA-positive women and one-hundred ANA-negative women matched for age and anti-Mullerian hormone (AMH) were included from a Reproductive Medical Central of a University Hospital. Sixty-one oocytes pick-up (OPU) cycles in ANA+ group and one-hundred OPU cycles in ANA− group were compared; 30 frozen embryo transfer cycles without treatment and 66 with prednisone plus HCQ treatment among ANA-positive women were compared. Results There was no statistical difference in number of retrieved oocytes (13.66 ± 7.71 vs 13.72 ± 7.23, p = .445), available embryos (5.23 ± 3.37 vs 5.47 ± 3.26, p = .347), high-quality embryos (3.64 ± 3.25 vs 3.70 ± 3.52, p = .832), and proportion of high-quality embryos (26.5% vs. 26.7%, p = .940). Biochemical pregnancy rate (33.3% vs. 68.2%, p < .05), clinical pregnancy rate (20.0% vs. 50.1%, p < .05), and implantation rate (5.6% vs. 31.8%, p < .05) were lower, and pregnancy loss rate (83.3% vs. 23.1%, p < .05) was higher in patients with treatment than no treatment. Conclusion The influence of ANA on number of retrieved oocytes, available embryos, high-quality embryos, and proration of high-quality embryos was not found. The treatment of prednisone plus HCQ may improve implantation rate, biochemical pregnancy rate, and clinical pregnancy rate, and reduce pregnancy loss rate in frozen embryo transfer outcomes for ANA-positive women.

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Does Estradiol Orally and Vaginally Administered Together Impact Live Birth and Neonatal Outcome in Artificial Frozen-thawed Embryo Transfer Cycles: A Retrospective Cohort Study

10.21203/rs.3.rs-42796/v1 ◽

2020 ◽

Author(s):

Yuan Liu ◽

Yixia Yang ◽

Xinting Zhou ◽

Yanmei Hu ◽

Yu Wu

Keyword(s):

Pregnancy Rate ◽

Preterm Delivery ◽

Embryo Transfer ◽

Live Birth ◽

Clinical Pregnancy ◽

Clinical Pregnancy Rate ◽

Delivery Rate ◽

Serum Estradiol ◽

Frozen Thawed Embryo Transfer ◽

Thawed Embryo Transfer

Abstract Background: Previous studies have demonstrated that newborns from fresh embryo transfer are with higher risk of small for gestation (SGA) rate than those from frozen-thawed embryo transfer (FET). It is suggested that supraphysiologic serum estradiol in controlled ovarian stimulation (COS)is one of reasons. Out study aims to investigate whether exogenous estradiol delivered regimens have an impact on live birth rate and singleton birthweight in hormone replacement (HRT)-FET cycles.Methods:This retrospective study involved patients undergoing their first FET with HRT endometrium preparation followed by two cleavage-staged embryos transfer, comparing orally and vaginal estradiol tablets (OVE) group versus oral estradiol tablets (OE) group from January 2015 to December 2018 at our center. A total of 792 patients fulfilled the criteria, including 282 live birth singletons. Live birth was the primary outcome. Secondary outcome included clinical pregnancy rate, singleton birthweight, large for gestational age (LGA) rate, SGA rate, preterm delivery rate. Results:Patients in OVE group achieved higher serum estradiol level with more days of estradiol treatment. No difference in live birth (Adjusted OR 1.327; 95%CI 0.982, 1.794, p=0.066) and clinical pregnancy rate (Adjusted OR 1.278; 95%CI 0.937, 1.743, p=0.121) was found between OVE and OE groups. Estradiol route did not affect birth weight (β=-30.962, SE=68.723, p=0.653), the odds of LGA (Adjusted OR 1.165; 95%CI 0.545, 2.490, p=0.694), the odds of SGA (Adjusted OR 0.569; 95%CI 0.096, 3.369, p=0.535) or the preterm delivery rate (Adjusted OR 0.969; 95%CI 0.292, 3.214, p=0.959).Conclusion:Estrogen orally and vaginally together did not have an impact on clinical outcomes and singleton birthweight compared to estrogen orally taken, but was accompanied with relative higher serum E2 level and potential maternal undesirable risks.

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RETROSPECTIVE STUDY TO COMPARE BETWEEN HYALURONAN ENRICHED MEDIUM AND BLASTOCYST TRANSFER MEDIUM FOR FROZEN EMBRYO TRANSFER( FET) AND ITS IMPACT ON CLINICAL PREGNANCY RATE (CPR) IN PATIENTS WITH 2 OR MORE IVF-ICSI CYCLE FAILURES

Fertility and Sterility ◽

10.1016/j.fertnstert.2020.08.527 ◽

2020 ◽

Vol 114 (3) ◽

pp. e185-e186

Author(s):

Fiyazur Rahman ◽

Ankita Gupta ◽

Dr. Rashmi Sharma ◽

Rohan Sharma ◽

Dr. Navkiran k Kaur

Keyword(s):

Retrospective Study ◽

Pregnancy Rate ◽

Embryo Transfer ◽

Clinical Pregnancy ◽

Frozen Embryo Transfer ◽

Clinical Pregnancy Rate ◽

Blastocyst Transfer ◽

Icsi Cycle

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Only extremely high peak serum estradiol adversely affects endometrial receptivity in IVF treatment

10.21203/rs.2.13893/v1 ◽

2019 ◽

Author(s):

Jianyuan Song ◽

Tingting Liao ◽

Liu Jiang ◽

Houming Su ◽

Licheng Ji ◽

...

Keyword(s):

Pregnancy Rate ◽

Embryo Transfer ◽

Implantation Rate ◽

Abortion Rate ◽

Clinical Pregnancy ◽

Clinical Pregnancy Rate ◽

Serum Estradiol ◽

Group 3 ◽

Group 2 ◽

Group 1

Abstract Purpose To explore the effect of different concentrations of peak serum estradiol levels on endometrial receptivity quantitatively. Methods In our reproductive medicine center, two best quality of day 3 (D3) embryos were transferred or frozen according to E 2 and progesterone levels on the day of human chorionic gonadotropin (hCG) administration and the number of oocytes retrieved. The remaining embryos were cultured to blastocyst stage and frozen. The patients were then categorized into three groups. The patients with frozen-thawed D3 embryo transfer in artificial cycles without blastocyst frozen served as group 1, those with fresh D3 embryo transfer without blastocyst frozen as group 2, and those with fresh D3 embryo transfer with blastocyst frozen as group 3. Each group was further stratified into 4 sub-groups according to E 2 levels on the day of hCG administration. Clinical pregnancy rate, implantation rate and abortion rate of frozen-thawed and fresh D3 embryo transfer were compared among the three groups in the same stratified E 2 levels. Results For E 2 <7,000 pg/mL, group 1 and group 2 had similar clinical pregnancy rate and implantation rate. But for E 2 ≥7,000 pg/mL, the clinical pregnancy rate in group 1 was significantly higher than in group 2 (p<0.05). For E 2 <7,000 pg/mL, pregnancy rate and implantation rate in group 1 were significantly lower than those in group 3 (P<0.05). But for E 2 ≥7,000 pg/mL, the pregnancy rate in group 1 was significantly higher than in group 3 (P<0.05). There was no significant difference in the abortion rate between group 1 and group 2, or between group 1 and group 3. Conclusions High serum E 2 concentration does not impair implantation and pregnancy rates unless exceeding a certain limit (e.g. 7,000 pg/mL) on the day of hCG administration. Since peak E 2 level was related to OHSS and adverse pregnancy outcomes, further study is needed to set a threshold peak E 2 level for fresh embryo transfer.

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Independent effect of body mass index on clinical pregnancy rate in single blastocyst frozen embryo transfer cycle in Asian women

Fertility Science and Research ◽

10.4103/fsr.fsr_14_19 ◽

2019 ◽

Vol 6 (2) ◽

pp. 115

Author(s):

TejashriM Shrotri ◽

Neeti Tiwari ◽

ShwetaMittal Gupta ◽

Ruma Satwik ◽

Gaurav Majumdar ◽

...

Keyword(s):

Body Mass Index ◽

Pregnancy Rate ◽

Embryo Transfer ◽

Body Mass ◽

Clinical Pregnancy ◽

Frozen Embryo Transfer ◽

Clinical Pregnancy Rate ◽

Independent Effect ◽

Asian Women

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Fresh versus frozen embryo transfer: a retrospective cohort study

International Journal of Reproduction Contraception Obstetrics and Gynecology ◽

10.18203/2320-1770.ijrcog20193814 ◽

2019 ◽

Vol 8 (9) ◽

pp. 3774

Author(s):

Raksha K. Shetty ◽

Purnima K. Nadkarni ◽

Pooja P. Singh ◽

Prabhakar Singh ◽

Aditi A. Nadkarni ◽

...

Keyword(s):

Cohort Study ◽

Pregnancy Rate ◽

Embryo Transfer ◽

Retrospective Cohort Study ◽

Retrospective Cohort ◽

Pregnancy Rates ◽

Clinical Pregnancy ◽

Frozen Embryo Transfer ◽

Clinical Pregnancy Rate ◽

Transfer Strategies

Background: Elective frozen embryo transfer (FET), has recently increased significantly with improvements in cryopreservation techniques. Observational studies and randomized controlled trials suggested that the endometrium in stimulated cycles is not optimally prepared for implantation; risk of ovarian hyperstimulation syndrome reduced and pregnancy rates increased following FET and perinatal outcomes are less affected after FET. However, the evidence is not unequivocal and recent randomised control trials challenge the use of elective FET for the general IVF population. Pregnancy rates were analysed in a cohort of patients undergoing embryo transfers.Methods: This was a retrospective cohort study of patients who underwent embryo transfers from April 2018 to March 2019 at study centre in Surat.175 cycles of embryo transfers (119 fresh and 56 frozen) were included in the study. Outcomes measured were positive pregnancy, clinical pregnancy and ongoing clinical pregnancy rates achieved in the IVF-ET cycles.Results: There were no statistically significant differences between positive pregnancy rate (54.6% versus 60.7%, Odds ratio (OR) 0.78; 95% Confidence Interval (CI) 0.41-1.49), clinical pregnancy rate (48.73% versus 57.14%, OR 0.52; 95% CI 0.1- 2.64) and ongoing clinical pregnancy rate (45.38% versus 51.78% OR 1.4; 95% CI 0.29 - 6.67) in fresh ET and FET cycles, respectively, p < 0.05 was considered statistically significant for all measures.Conclusions: Despite the observed higher rates of positive biochemical, clinical and ongoing clinical pregnancy per transfer in the FET cohort, these did not reach statistical significance. Thus, both transfer strategies are reasonable options, although there is a trend favouring the freeze-all strategy.

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