Background: Cystic echinococcosis (CE) is commonly located in the liver
and lungs of affected hosts. Surgical management is one of the best
choices for the treatment of hydatidosis and using effective scolicidal
agents during hydatid surgery is essential to prevent secondary
infection. The present study was designed to investigate the in vitro
scolicidal activity of methanol extract of Sideritis perfoliata against
the protoscoleces of hydatid cysts. Methods: The protoscoleces were
collected from slaughtered livestock in Adiyaman and the effect of three
concentrations of the aerial part extract of S. perfoliata (0.1mg/ml,
0.2mg/ml, and 0.4mg/ml) was assessed over three different exposure
periods. All tests were carried in dublicate. Finally, the mortality of
protoscoleces was assessed by the eosin exclusion test (0.1% eosin
staining). Methanol extract of S. perfoliata was assessed by Liquid
Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). Results:
The results showed that the scolicidal effect of this extract at
exposure periods of 10, 20, and 30 min was 29.6, 32.5, and 43.6% at
concentrations of 0.1mg/ml, 37.8, 50, and 58.1% at concentration of
0.2mg/ml and finally 57.9, 71.8, and 79.1% at concentration of
0.4mg/ml, respectively; indicating that the extract requiring a further
time to display a potent protoscolicidal effects. Some phenolic acids
such as fumaric acid (260,13mg/L), syringic acid (27,92mg/L) and caffeic
acid (26,84mg/L) and a flavonoid, luteolin (11,23 mg/L) were detected in
high concentrations. Conclusions: The present study has demonstrated
that the methanol extract of S. perfoliata has high scolicidal power in
vitro, although the low concentration of plant extract may provide a
base for future treatment of hydatid cysts. However, more research on
the in vivo efficacy of S. perfoliata extract and its potential side
effects is recommended.
IN VITRO ANTIOXIDANT AND IN VIVO ANTI-INFLAMMATORY ACTIVITY OF THE AERIAL PART OF BLUMEA ERIANTHA DC
