scholarly journals GC-MS Analysis of Bioactive Phytochemicals in Methanol Extract of Aerial Part and Callus of Dipterygium glaucum Decne

2019 ◽  
Vol 11 (5) ◽  
pp. 1055-1063
Author(s):  
Choudhary Choudhary D ◽  
Shekhawat Shekhawat JK ◽  
Kataria Kataria V
Keyword(s):  
Aerial Part ◽  
Methanol Extract ◽  
Ms Analysis ◽  
Bioactive Phytochemicals

scholarly journals PRELIMINARY SCREENING OF PHYTOCHEMICAL CONSTITUENTS, ANTIOXIDANT, AND ANTI-BACTERIAL ACTIVITIES IN THE METHANOLIC LEAF EXTRACT OF MELIOSMA SIMPLICIFOLIA (L.)

2021 ◽  
pp. 100-104
Author(s):  
PAVITHRA S ◽  
SEKAR T
Keyword(s):  
Leaf Extract ◽  
Methanol Extract ◽  
Antioxidant Activities ◽  
Reducing Power ◽  
Hot Water ◽  
Phytochemical Analysis ◽  
Scavenging Activity ◽  
Leaf Extracts ◽  
Ms Analysis ◽  
Dpph Scavenging

Objective: In the present study is investigated of phytochemicals and antioxidant activities of the leaf extracts from Meliosma simplicifolia (L.). Methods: The seaweed sample was subjected to extraction with solvents of different polarities (hot water, methanol, acetone, chloroform, and petroleum ether) and screened for phytochemicals according to standard methods. The ability of the plant extract to act as hydrogen/electrons donor or scavenger of radicals was determined by in vitro antioxidant assays using 2,2-diphenyl-2-picryl-hydrazyl free radical (DPPH) scavenging, reducing power assay, and superoxide radical (O2•) scavenging activity. The ICPMS and GC-MS analysis of the methanol leaf extract of M. simplicifolia was revealed the presence of antibacterial. Results: Phytochemical analysis revealed the presence of Preliminary metabolites Quantitative studies of estimated phenol, flavonoid and tannin, as for the methanol extract of stem showed the highest content of phenolic compounds (40.71±0.94 GAE mg/100). Antioxidant activities were concluded the estimation M. simplicifolia leaf for as followed the studies. In leaf the highest DPPH scavenging activity (132.3 μg/ml), ICP-MS analysis of the leaf extract showed the presence minerals such as Mg, Fe, Cu, Na, and Zn in excess. The leaf extract of the plant was also tested for its antibacterial activity and results confirmed that it has potential activity. Conclusion: The preliminary studies in the methanol extract of the leaf of M. simplicifolia are suggestive of the antibacterial potentials of the methanol extract of leaves of M. simplicifolia.

scholarly journals Melanogenesis Inhibitory Activity of Diterpenoid and Triterpenoid Constituents from the Aerial Part of Isodon trichocarpus

2017 ◽  
Vol 12 (8) ◽  
pp. 1934578X1701200
Author(s):  
Yoshiaki Manse ◽  
Kiyofumi Ninomiya ◽  
Akane Okazaki ◽  
Eriko Okada-Nishida ◽  
Takahiro Imagawa ◽  
...  
Keyword(s):  
Inhibitory Activity ◽  
Aerial Part ◽  
Methanol Extract ◽  
Inhibitory Effect ◽  
B16 Melanoma ◽  
Related Protein ◽  
Inhibitory Effects

A methanol extract from the aerial part of Isodon trichocarpus (Labiatae) demonstrated inhibitory effects on melanogenesis in theophylline-stimulated murine B16 melanoma 4A5 cells (IC50 = 1.6 μg/mL). From the extract, nine diterpenoids (1–9) and four triterpenoids (10–13) were isolated. Among the isolates, enmein (1, IC50 = 0.22 μM), isodocarpin (2, 0.19 μM), nodosin (4, 0.46 μM), and oridonin (6, 0.90 μM) showed an inhibitory effect without notable cytotoxicity at the effective concentrations. These diterpenoids (1, 2, 4, and 6) inhibited the expression of tyrosinase, tyrosine-related protein (TRP)-1, and TRP-2 mRNA, which could be the mechanism of melanogenesis inhibitory activity.

scholarly journals Phytochemical Screening, Biological Studiesand GC-MS Analysis of Extract of Biden pilosa L

2019 ◽  
Vol 5 (1) ◽  
pp. 79-93
Author(s):  
Dharma Prasad Khanal ◽  
Rupa Rana ◽  
Bechan Raut ◽  
Rabindra Prasad Dhakal
Keyword(s):  
Ethyl Acetate ◽  
Aerial Part ◽  
Inflammatory Activity ◽  
Alcoholic Extract ◽  
Acute Oral Toxicity ◽  
Oral Toxicity ◽  
Bidens Pilosa ◽  
Aerial Parts ◽  
Ms Analysis ◽  
Anti Inflammatory

Objective: The aim of the research work was to carry out the extraction of areal parts of Biden pilosa L by hydroalcholic and Hexane, ethyl acetate and acetone mixture followed by qualitative phytochemical analysis, acute oral toxicity test, anti-inflammatory test and GC-MS analysis of the extracts. Method: The hydro-alcoholic and HEA(n-hexane, ethyl acetate and acetone) extraction was done from aerial parts using ethanol and water in the ratio 70:30 and n-hexane, ethyl acetate and acetone in the ratio of 1:1:1 (HEA extract)  respectively. Acute oral toxicity testwas performed OECD guidelines. The single spot in TLC was obtained using n-hexane as solvent for HEA fraction and finally phytocomponents were identified by GC-MS present in that spot.In vitro anti-inflammatory activity was performed by human RBC membrane stabilization method. Result: The phytochemical test results obtained indicate that hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. Both extracts showed significant in vitro anti-inflammatory activity in a concentration dependent manner. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds from partially separated extract from TLC plates. Conclusion: Hydro-alcoholic extract of aerial part of Bidens pilosa L. possess alkaloids, tannins, terpenoids and saponins whereas HEA extract possess alkaloids, flavonoids, tannins. Both hydro-alcoholic and HEA extracts were found to be safe up to the dose of 2000 mg/kg BW of the mice. The GC-MS analysis of HEA extract of aerial parts showed the presence of the sixteen different compounds.

scholarly journals In vitro neutralization of Eachis carinatus and Naja naja venom by Canthium parviflorum and its GC-MS analysis

2016 ◽  
Vol 11 (4) ◽  
pp. 920 ◽  
Author(s):  
Vineetha M. Shrikanth ◽  
Bhavya Janardhan ◽  
Sunil S. More
Keyword(s):  
Phospholipase A2 ◽  
Enzyme Activities ◽  
Methanol Extract ◽  
Video Clip ◽  
Hydrolytic Enzymes ◽  
Root Extract ◽  
Site Specific ◽  
Naja Naja ◽  
Ms Analysis

<p class="Abstract"><em>Canthium </em>parviflorum is used in the traditional therapy to treat snakebite victims. In the present study, partially purified methanol root extract of <em>C. </em>parviflorum was used for the neutralization of Eachis carinatus and <em>Naja naja</em> venom. The extract inhibited <em>in vitro</em> phosphomonoesterase, phosphodiesterase, acetylcholinesterase, hyaluronidase, protease, phospholipase A<sub>2 </sub>and 5’nucleotidase activities of both venoms. One of the promising band exhibited neutralization of all the<em> in vitro</em> enzyme activities and was further subjected to GC-MS analysis which revealed the presence of eight active phytocompounds. These phytochemicals might be responsible for <em>in vitro</em> enzyme neutralization. Methanol extract possesses potent active phytochemicals against the site specific toxins and hydrolytic enzymes analyzed.</p><p class="Abstract"><strong>Video Clip of Methodology</strong>:</p><p class="Abstract"><a href="https://youtube.com/v/oSLdFcfaJxU">Phospholipase A2 assay</a>: 1 min 36 sec</p>

scholarly journals GC-MS Analysis of Methanol and Ethyl Acetate Extract of fruits of Sphaeranthus indicus

2019 ◽  
Vol 9 (2) ◽  
pp. 28-30
Author(s):  
Kalpana Garg ◽  
Birendra Shrivastava ◽  
Anurag Bhargava
Keyword(s):  
Mass Spectrometry ◽  
Gas Chromatography ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Ethyl Acetate Extract ◽  
Gas Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry ◽  
Ms Analysis ◽  
Sphaeranthus Indicus

Introduction: To identify the various phytoconstituents present in the plant Sphaeranthus indicus by using gas chromatography and mass spectrometry. Meth­ods: The fruits of Sphaeranthus indicus were extracted with Different solvents of increasing polarity. The methanol and ethylacetate extract were subjected to GCMS analysis to detect the phytoconstituents. Results: Totally 26 compounds were identified. Among these 13 constituents in methanol extract and 13 constituents in ethylacetate extract were identified during the GC-MS analysis. Stigmasterol and lupeol which were identified in the plant is considered to have antiarthritic properties. Keywords: Sphaeranthus indicus, Gas chromatography, Mass spectrometry.

scholarly journals Identification of Bioactive Compounds from Alphonsea madraspatana Leaves against Multi Drug Resistance Bacteria

2021 ◽  
Vol 33 (6) ◽  
pp. 1273-1279
Author(s):  
A. Sahu ◽  
S.S. Swain ◽  
D. Pradhan ◽  
D.K. Sahu ◽  
P. Biswasroy ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Polar Compounds ◽  
Hexane Extract ◽  
Multi Drug Resistance ◽  
Multiple Drug ◽  
Antimicrobial Compounds ◽  
Polar Components ◽  
Rp Hplc ◽  
Ms Analysis ◽  
Gradient Technique

In this study, the antimicrobial compounds present in leaves of Alphonsea madraspatana was identified by using RP-HPLC, LC/MS and GC/MS and found to be active against multiple drug resistant (MDR) bacteria e.g. Escherichia coli, Staphylococcus aureus and Pseudomonas aeruginosa. Extraction of dried plant leaves was performed by solvent gradient technique. Antimicrobial study was performed with each extract. n-Hexane and methanol extracts were further subjected to analysis for identification of potent antimicrobial compounds due to their better antimicrobial activity as compared to remaining extracts. The methanolic extract was more active than n-hexane extract. As n-hexane extract contains non-polar compounds, it was subjected to GC-MS analysis and methanol extract was subjected to RPHPLC followed by LC/MS due to presence of polar components. Among the isolated compounds, four compounds were identified as potent antimicrobial. RP-HPLC and LC/MS analysis of methanol extract also ensured the presence of four potent antimicrobial compounds as follows: (i) kaempferol-3-O-rotinoside-orhamnoside (m/z 748), (ii) 3-hydroxypropylglucosinolate (m/z 377), (iii) luteolin-7-O-glucoside (m/z 448) and (iv) genistein-7-O-glycoside (m/z 432). Leaves of this plant may be used as potent antibacterial agents due to presence of antimicrobial compounds.

scholarly journals In Vitro Scolicidal Effects of Sideritis perfoliata Aerial Part Extract against the Protoscoleces of Echinococcus granulosus

2021 ◽  
Author(s):  
TUNCAY Çelik ◽  
Muhittin Önderci ◽  
Mustafa Pehlivan ◽  
Önder Yumrutaş
Keyword(s):  
Mass Spectrometry ◽  
Aerial Part ◽  
Methanol Extract ◽  
Secondary Infection ◽  
Syringic Acid ◽  
Hydatid Cysts ◽  
High Concentrations ◽  
Mass Spectrometry Mass Spectrometry

Background: Cystic echinococcosis (CE) is commonly located in the liver and lungs of affected hosts. Surgical management is one of the best choices for the treatment of hydatidosis and using effective scolicidal agents during hydatid surgery is essential to prevent secondary infection. The present study was designed to investigate the in vitro scolicidal activity of methanol extract of Sideritis perfoliata against the protoscoleces of hydatid cysts. Methods: The protoscoleces were collected from slaughtered livestock in Adiyaman and the effect of three concentrations of the aerial part extract of S. perfoliata (0.1mg/ml, 0.2mg/ml, and 0.4mg/ml) was assessed over three different exposure periods. All tests were carried in dublicate. Finally, the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining). Methanol extract of S. perfoliata was assessed by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). Results: The results showed that the scolicidal effect of this extract at exposure periods of 10, 20, and 30 min was 29.6, 32.5, and 43.6% at concentrations of 0.1mg/ml, 37.8, 50, and 58.1% at concentration of 0.2mg/ml and finally 57.9, 71.8, and 79.1% at concentration of 0.4mg/ml, respectively; indicating that the extract requiring a further time to display a potent protoscolicidal effects. Some phenolic acids such as fumaric acid (260,13mg/L), syringic acid (27,92mg/L) and caffeic acid (26,84mg/L) and a flavonoid, luteolin (11,23 mg/L) were detected in high concentrations. Conclusions: The present study has demonstrated that the methanol extract of S. perfoliata has high scolicidal power in vitro, although the low concentration of plant extract may provide a base for future treatment of hydatid cysts. However, more research on the in vivo efficacy of S. perfoliata extract and its potential side effects is recommended.

Sign in / Sign up

Export Citation Format

Share Document