Use of acetic and citric acids to inhibit Escherichia coli O157:H7, Salmonella Typhimurium and Staphylococcus aureus in tabbouleh salad

2018 ◽  
Vol 73 ◽  
pp. 61-66 ◽  
Author(s):  
Walid M. Al-Rousan ◽  
Amin N. Olaimat ◽  
Tareq M. Osaili ◽  
Anas A. Al-Nabulsi ◽  
Radwan Y. Ajo ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Salmonella Typhimurium ◽  
Escherichia Coli O157 ◽  
And Staphylococcus Aureus

scholarly journals Attachment and Colonization by Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica subsp. enterica serovar Typhimurium, and Staphylococcus aureus on Stone Fruit Surfaces and Survival through a Simulated Commercial Export Chain

2010 ◽  
Vol 73 (7) ◽  
pp. 1247-1256 ◽  
Author(s):  
STACEY COLLIGNON ◽  
LISE KORSTEN
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Stone Fruit ◽  
Escherichia Coli O157 ◽  
Infectious Dose ◽  
E Coli ◽  
Serovar Typhimurium ◽  
And Staphylococcus Aureus

The ability of the foodborne pathogens Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica subsp. enterica serovar Typhimurium, and Staphylococcus aureus to attach, colonize, and survive on stone fruit surfaces was investigated. Fifty microliters of bacterial suspension was spot inoculated onto the sterile intact fructoplane of whole peaches and plums. Minimum time required for initial adhesion and attachment was recorded for different surface contact times. Surface colonization patterns of the four pathogens and survival under simulated commercial export conditions also were evaluated. L. monocytogenes and Salmonella Typhimurium attached immediately to stone fruit surfaces. E. coli O157:H7 and S. aureus were visibly attached after 30 s and 1 h, respectively, of direct exposure. Holding freshly harvested stone fruit at 0.5°C to simulate cold storage conditions significantly lowered the titer of E. coli O157:H7 on plums and the titers of L. monocytogenes and Salmonella Typhimurium on stone fruit. E. coli O157:H7 and L. monocytogenes at a low inoculum level and S. aureus and Salmonella Typhimurium at high and low levels did not survive the simulated export chain conditions at titers that exceeded the minimum infectious dose. However, E. coli O157:H7 and L. monocytogenes were able to survive on stone fruit surfaces when inoculated at an artificially high level. In this case, the final titer at the end of the supply chain was higher than the infectious dose. In this laboratory experiment, E. coli O157:H7, L. monocytogenes, Salmonella Typhimurium, and S. aureus at potential natural contamination levels were unable to survive simulated export conditions.

scholarly journals ATIVIDADE ANTIMICROBIANA DE MICRORGANISMOS ISOLADOS DE GRÃOS DE KEFIR

2018 ◽  
Vol 19 (0) ◽  
Author(s):  
Priscila Alves Dias ◽  
Daiani Teixeira Silva ◽  
Cláudio Dias Timm
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Salmonella Typhimurium ◽  
Salmonella Enterica ◽  
Escherichia Coli O157 ◽  
E Coli

Resumo Kefir é o produto da fermentação do leite pelos grãos de kefir. Esses grãos contêm uma mistura simbiótica de bactérias e leveduras imersas em uma matriz composta de polissacarídeos e proteínas. Muitos benefícios à saúde humana têm sido atribuídos ao kefir, incluindo atividade antimicrobiana contra bactérias Gram positivas e Gram negativas. A atividade antimicrobiana de 60 microrganismos isolados de grãos de kefir, frente à Escherichia coli O157:H7, Salmonella enterica subsp. enterica sorotipos Typhimurium e Enteritidis, Staphylococcus aureus e Listeria monocytogenes, foi estudada através do teste do antagonismo. A ação antimicrobiana dos sobrenadantes das bactérias ácido-lácticas que apresentaram atividade no teste do antagonismo foi testada. O experimento foi repetido usando sobrenadantes com pH neutralizado. Salmonella Typhimurium e Enteritidis sobreviveram por 24 horas no kefir em fermentação. E. coli O157:H7, S. aureus e L. monocytogenes foram recuperados até 72 horas após o início da fermentação. Todos os isolados apresentaram atividade antimicrobiana contra pelo menos um dos patógenos usados no teste do antagonismo. Sobrenadantes de 25 isolados apresentaram atividade inibitória e três mantiveram essa atividade com pH neutralizado. As bactérias patogênicas estudadas sobreviveram por tempo superior àquele normalmente utilizado para a fermentação do kefir artesanal, o que caracteriza perigo em potencial para o consumidor quando a matéria-prima não apresentar segurança sanitária. Lactobacillus isolados de grãos de kefir apresentam atividade antimicrobiana contra cepas de E. coli O157:H7, Salmonella sorotipos Typhimurium e Enteritidis, S. aureus e L. monocytogenes além daquela exercida pela diminuição do pH.

Comparison of Four Commercial DNA Extraction Kits for PCR Detection of Listeria monocytogenes, Salmonella, Escherichia coli O157:H7, and Staphylococcus aureus in Fresh, Minimally Processed Vegetables

2008 ◽  
Vol 71 (10) ◽  
pp. 2110-2114 ◽  
Author(s):  
P. ELIZAQUÍVEL ◽  
R. AZNAR
Keyword(s):  
Escherichia Coli ◽  
Staphylococcus Aureus ◽  
Listeria Monocytogenes ◽  
Dna Extraction ◽  
Pcr Detection ◽  
Escherichia Coli O157 ◽  
Minimally Processed ◽  
Green Pepper ◽  
Minimally Processed Vegetables ◽  
And Staphylococcus Aureus

Four commercial DNA extraction methods, PrepMan Ultra (Applied Biosystems), InstaGene Matrix (BioRad), DNeasy Tissue kit (Qiagen), and UltraClean (MoBio), were tested for PCR detection of Listeria monocytogenes, Escherichia coli O157: H7, Salmonella, and Staphylococcus aureus in fresh, minimally processed vegetables. For comparative purposes, sensitivity assays with specific PCRs were carried out after DNA extraction with the four methods in green pepper, broccoli, and onion artificially inoculated with the four pathogens separately. As confirmed by statistical analysis, the DNeasy Tissue kit rendered the highest sensitivity values in the three matrices assayed for Salmonella, L. monocytogenes, and E. coli O157:H7 and in onion for S. aureus. Despite being the most expensive of the methods compared, the DNeasy Tissue Kit can be successfully applied for any of the four most commonly studied pathogens, thus saving time and overall reducing the cost of the analysis.

Sign in / Sign up

Export Citation Format

Share Document