Assessment of Algerian sorghum protein quality [Sorghum bicolor (L.) Moench] using amino acid analysis and in vitro pepsin digestibility

Foi avaliado o desempenho de novilhos de corte, durante a fase de recria, em capim-elefante (Pennisetum purpureum Schum.) cv. Taiwan A-146, papuã (Brachiaria plantaginea (Link) Hitchc), sorgo forrageiro (Sorghum bicolor (L.) Moench) cv. AG 2501C e milheto (Pennisetum americanum (L.) Leeke) cv. comum. O período total de pastejo foi de 143 dias para a pastagem de capim-elefante e 98 dias para as demais pastagens. O sistema de pastejo foi o contínuo com lotação variável. O teor de proteína bruta na massa de forragem foi de 5,43; 10,08; 9,95; e 10,58% e a digestibilidade in vitro da matéria seca, de 50,93; 55,85; 54,56; e 54,81% para capim-elefante, papuã, sorgo e milheto, respectivamente. Houve diferença significativa no ganho de peso médio diário, que foi de 0,928; 1,054; 1,121; e 1,188 kg, para o capim-elefante, papuã, sorgo e milheto, respectivamente. Não houve diferença significativa na carga animal entre as pastagens, sendo de 1682, 1634, 1389 e 1514 kg de PV/ha, e no ganho de peso vivo, que foi de 774, 668, 570 e 640 kg de PV/ha para as pastagens de capim-elefante, papuã, sorgo e milheto, respectivamente. A utilização de pastagens cultivadas de verão, manejadas corretamente, permite altos ganhos de peso por animal e por área, constituindo-se em uma excelente alternativa para intensificar a produção de bovinos de corte.

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Comparison of Interlaboratory Variation in Amino Acid Analysis and Rat Growth Assays for Evaluating Protein Quality

Journal of AOAC INTERNATIONAL ◽

10.1093/jaoac/68.1.52 ◽

1985 ◽

Vol 68 (1) ◽

pp. 52-56 ◽

Cited By ~ 1

Author(s):

Ghulam Sarwar ◽

Robert Blair ◽

Mendel Friedman ◽

Michael R Gumbmann ◽

Ross L Hackler ◽

...

Keyword(s):

Amino Acids ◽

Amino Acid ◽

Amino Acid Analysis ◽

Protein Quality ◽

Essential Amino Acids ◽

Quality Indices ◽

Protein Ratio ◽

Protein Sources ◽

Rat Growth ◽

Positive Correlations

Abstract Estimates of inter- and intralaboratory variation of protein efficiency ratio (PER), relative PER (RPER), net protein ratio (NPR), relative NPR (RNPR), and nitrogen utilization (NU) were compared with those of amino acid analysis in the same batches of 7 protein sources (ANRC casein, egg white solids, minced beef, soy assay protein, rapeseed protein concentrate, pea flour, and whole wheat flour). Interlaboratory variation (estimated as between-laboratories coefficients of variation, CV) of NPR and RNPR (up to 6.0%) was lower than that of PER (up to 20.2%) and RPER (up to 18.5%). The interlaboratory determination of NPR and RNPR was also more reproducible than that of most essential amino acids (CV up to 10.0%), especially tryptophan (CV up to 23.7%), cystine (CV up to 17.6%), and methionine (CV up to 16.1%). Intralaboratory variation (estimated as within-laboratories CV) of amino acid analysis (up to 4.7%), however, was comparable to that of protein quality indices in most protein sources (up to 6.0%). The significant (P <0.01) positive correlations (r = 0.68-0.74) between amino acid scores and protein quality indices based on rat growth were further improved when amino acid scores were corrected for digestibility of protein (r = 0.73-0.78) or individual amino acids (r = 0.79- 0.82).

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