Untargeted metabolite profiling and phytochemical analysis of Micromeria fruticosa L. (Lamiaceae) leaves

Mitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially localized 3XHA epitope tag (MITO-Tag) for the fast isolation of mitochondria from cultured cells to generate MITO-Tag Mice. Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific immunoisolation of mitochondria from tissues, which we verified using a combination of proteomic and metabolomic approaches. Using MITO-Tag Mice and targeted and untargeted metabolite profiling, we identified changes during fasted and refed conditions in a diverse array of mitochondrial metabolites in hepatocytes and found metabolites that behaved differently at the mitochondrial versus whole-tissue level. MITO-Tag Mice should have utility for studying mitochondrial physiology, and our strategy should be generally applicable for studying other mammalian organelles in specific cell types in vivo.

Download Full-text

MITO-Tag Mice enable rapid isolation and multimodal profiling of mitochondria from specific cell types in vivo

10.1101/425454 ◽

2018 ◽

Author(s):

Erol Can Bayraktar ◽

Lou Baudrier ◽

Ceren Özerdem ◽

Caroline A. Lewis ◽

Sze Ham Chan ◽

...

Keyword(s):

Metabolite Profiling ◽

Cultured Cells ◽

Transgenic Animals ◽

Cell Types ◽

Tissue Level ◽

Specific Cell ◽

Mammalian Tissues ◽

Cell Type Specific ◽

Untargeted Metabolite Profiling

ABSTRACTMitochondria are metabolic organelles that are essential for mammalian life, but the dynamics of mitochondrial metabolism within mammalian tissues in vivo remains incompletely understood. While whole-tissue metabolite profiling has been useful for studying metabolism in vivo, such an approach lacks resolution at the cellular and subcellular level. In vivo methods for interrogating organellar metabolites in specific cell-types within mammalian tissues have been limited. To address this, we built on prior work in which we exploited a mitochondrially-localized 3XHA epitope-tag (“MITO-Tag”) for the fast isolation of mitochondria from cultured cells to now generate “MITO-Tag Mice.” Affording spatiotemporal control over MITO-Tag expression, these transgenic animals enable the rapid, cell-type-specific immunoisolation of mitochondria from tissues, which we verified using a combination of proteomic and metabolomic approaches. Using MITO-Tag Mice and targeted and untargeted metabolite profiling, we identified changes during fasted and refed conditions in a diverse array of mitochondrial metabolites in hepatocytes and found metabolites that behaved differently at the mitochondrial versus whole-tissue level. MITO-Tag Mice should have utility for studying mitochondrial physiology and our strategy should be generally applicable for studying other mammalian organelles in specific cell-types in vivo.

Download Full-text

Untargeted metabolite profiling reveals that nitric oxide bioynthesis is an endogenous modulator of carotenoid biosynthesis in Deinococcus radiodurans and is required for extreme ionizing radiation resistance

Archives of Biochemistry and Biophysics ◽

10.1016/j.abb.2015.10.010 ◽

2016 ◽

Vol 589 ◽

pp. 38-52 ◽

Cited By ~ 9

Author(s):

Alex Hansler ◽

Qiuying Chen ◽

Yuliang Ma ◽

Steven S. Gross

Keyword(s):

Nitric Oxide ◽

Ionizing Radiation ◽

Radiation Resistance ◽

Metabolite Profiling ◽

Deinococcus Radiodurans ◽

Carotenoid Biosynthesis ◽

Endogenous Modulator ◽

Untargeted Metabolite Profiling ◽

Ionizing Radiation Resistance

Download Full-text

Targeted and untargeted-metabolite profiling to track the compositional integrity of ginger during processing using digitally-enhanced HPTLC pattern recognition analysis

Journal of Chromatography B ◽

10.1016/j.jchromb.2018.02.020 ◽

2018 ◽

Vol 1080 ◽

pp. 59-63 ◽

Cited By ~ 2

Author(s):

Reham S. Ibrahim ◽

Hoda Fathy

Keyword(s):

Pattern Recognition ◽

Metabolite Profiling ◽

Untargeted Metabolite Profiling ◽

Pattern Recognition Analysis ◽

Digitally Enhanced

Download Full-text

Untargeted metabolite profiling for koji-fermentative bioprocess unravels the effects of varying substrate types and microbial inocula

Food Chemistry ◽

10.1016/j.foodchem.2018.05.048 ◽

2018 ◽

Vol 266 ◽

pp. 161-169 ◽

Cited By ~ 8

Author(s):

Han Sol Seo ◽

Sunmin Lee ◽

Digar Singh ◽

Hye Won Shin ◽

Sun A Cho ◽

...

Keyword(s):

Metabolite Profiling ◽

Untargeted Metabolite Profiling ◽

Microbial Inocula ◽

Substrate Types

Download Full-text

Untargeted metabolite profiling of liver in mice exposed to 2‐methylfuran

Journal of Food Science ◽

10.1111/1750-3841.15549 ◽

2020 ◽

Author(s):

Chuanqin Hu ◽

Ren Li ◽

Jiahui Wang ◽

Yingli Liu ◽

Jing Wang ◽

...

Keyword(s):

Metabolite Profiling ◽

Untargeted Metabolite Profiling

Download Full-text

Untargeted Metabolite Profiling for Screening Bioactive Compounds in Digestate of Manure under Anaerobic Digestion

Water ◽

10.3390/w11112420 ◽

2019 ◽

Vol 11 (11) ◽

pp. 2420 ◽

Cited By ~ 2

Author(s):

Jiaxin Lu ◽

Atif Muhmood ◽

Wojciech Czekała ◽

Jakub Mazurkiewicz ◽

Jacek Dach ◽

...

Keyword(s):

Anaerobic Digestion ◽

Bioactive Compounds ◽

Metabolic Pathways ◽

Metabolite Profiling ◽

Swine Manure ◽

Chicken Manure ◽

Chemical Characteristics ◽

Chemical Profiling ◽

Different Types ◽

Untargeted Metabolite Profiling

Untargeted metabolite profiling was performed on chicken manure (CHM), swine manure (SM), cattle manure (CM), and their respective digestate by XCMS coupled with MetaboAnalyst programs. Through global chemical profiling, the chemical characteristics of different digestates and types of manure were displayed during the anaerobic digestion (AD) process. As the feed for AD, CM had less easily-degradable organics, SM contained the least O-alkyls and anomerics of carbohydrates, and CHM exhibited relatively lower bio-stability. The derived metabolite pathways of different manure during the AD process were identified by MetaboAnalyst. Twelve, 8, and 5 metabolic pathways were affected by the AD process in CHM, SM, and CM, respectively. Furthermore, bioactive compounds of digestate were detected, such as amino acids (L-arginine, L-ornithine, L-cysteine, and L-aspartate), hormones (L-adrenaline, 19-hydroxy androstenedione, and estrone), alkaloids (tryptamine and N-methyltyramine), and vitamin B5, in different types of manure and their digestates. The combination of XCMS and MetaboAnalyst programs can be an effective strategy for metabolite profiling of manure and its anaerobic digestate under different situations.

Download Full-text