Effect of salt promote the muscle triglyceride hydrolysis during dry-salting by inducing the phosphorylation of adipose tissue triglyceride lipase (ATGL) and hormone-sensitive lipase (HSL) and lipid droplets splitting

2020 ◽  
Vol 327 ◽  
pp. 127061
Author(s):  
Shilin Zhao ◽  
Lichao He ◽  
Min Zhang ◽  
Xiaojie Liu ◽  
Guofeng Jin
Keyword(s):  
Adipose Tissue ◽  
Lipid Droplets ◽  
Hormone Sensitive Lipase ◽  
Triglyceride Lipase ◽  
Triglyceride Hydrolysis ◽  
Adipose Tissue Triglyceride ◽  
Hormone Sensitive ◽  
Muscle Triglyceride

scholarly journals Glucocorticoids antagonize tumor necrosis factor-α-stimulated lipolysis and resistance to the antilipolytic effect of insulin in human adipocytes

2012 ◽  
Vol 303 (9) ◽  
pp. E1126-E1133 ◽  
Author(s):  
Mi-Jeong Lee ◽  
Susan K. Fried
Keyword(s):  
Adipose Tissue ◽  
Cell Types ◽  
Hormone Sensitive Lipase ◽  
Human Adipocytes ◽  
Adipose Tissue Triglyceride ◽  
Hormone Sensitive ◽  
Phosphodiesterase 3B ◽  
High Concentrations ◽  
Factor Α ◽  
Tnf Induction

High concentrations of TNF within obese adipose tissue increase basal lipolysis and antagonize insulin signaling. Adipocytes of the obese are also exposed to elevated levels of glucocorticoids (GCs), which antagonize TNF actions in many cell types. We tested the hypothesis that TNF decreases sensitivity to the antilipolytic effect of insulin and that GCs antagonize this effect in differentiated human adipocytes. Lipolysis and expression levels of lipolytic proteins were measured after treating adipocytes with TNF, dexamethasone (DEX), or DEX + TNF for up to 48 h. TNF not only increased basal lipolysis, it caused resistance to the antilipolytic effects of insulin in human adipocytes. DEX alone did not significantly affect lipolysis. Cotreatment with DEX blocked TNF induction of basal lipolysis and insulin resistance by antagonizing TNF stimulation of PKA-mediated phosphorylation of hormone-sensitive lipase (HSL) at Ser563 and Ser660 and perilipin. TNF did not affect perilipin, HSL, or phosphodiesterase-3B mass but paradoxically suppressed adipose tissue triglyceride lipase expression, and this effect was blocked by DEX. The extent to which GCs can restrain the lipolytic actions of TNF may both diminish the potentially deleterious effects of excess lipolysis and contribute to fat accumulation in obesity.

scholarly journals Absence of cardiac lipid accumulation in transgenic mice with heart-specific HSL overexpression

2001 ◽  
Vol 281 (4) ◽  
pp. E857-E866 ◽  
Author(s):  
Jinya Suzuki ◽  
Wen-Jun Shen ◽  
Brett D. Nelson ◽  
Shailja Patel ◽  
Jacques H. Veerkamp ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Adipose Tissue ◽  
Lipid Droplets ◽  
Cytoskeletal Proteins ◽  
Hormone Sensitive Lipase ◽  
Acid Oxidation ◽  
Histocompatibility Antigens ◽  
Altered Expression ◽  
Hormone Sensitive ◽  
Responsive Element

Hormone-sensitive lipase (HSL) hydrolyzes triglyceride (TG) in adipose tissue. HSL is also expressed in heart. To explore the actions of cardiac HSL, heart-specific, tetracycline (Tc)-controlled HSL-overexpressing mice were generated. Tc-responsive element-HSL transgenic (Tg) mice were generated and crossed with myosin heavy chain (MHC)α-tTA Tg mice, which express the Tc-responsive transactivator (tTA) in the heart. The double-Tg mice (MHC-HSL) were maintained with doxycycline (Dox) to suppress Tg HSL. Upon removal of Dox, cardiac HSL activity and protein increased 12- and 8-fold, respectively, and the expression was heart specific. Although cardiac TG content increased twofold in control mice after an overnight fast, it did not increase in HSL-induced mice. Electron microscopy showed numerous lipid droplets in the myocardium of fasted control mice, whereas fasted HSL-induced mice showed virtually no droplets. Microarray analysis showed altered expression of cardiac genes for fatty acid oxidation, transcription factors, signaling molecules, cytoskeletal proteins, and histocompatibility antigens in HSL-induced mice. Thus cardiac HSL plays a role in controlling accumulation of triglyceride droplets and can affect the expression of a number of cardiac genes.

Lipase regulation of muscle triglyceride hydrolysis

1990 ◽  
Vol 69 (5) ◽  
pp. 1571-1577 ◽  
Author(s):  
L. B. Oscai ◽  
D. A. Essig ◽  
W. K. Palmer
Keyword(s):  
Direct Evidence ◽  
Hormone Sensitive Lipase ◽  
Triglyceride Hydrolysis ◽  
Energy Demands ◽  
Hormone Sensitive ◽  
Intramuscular Triglyceride ◽  
And Function ◽  
Parenchymal Cells ◽  
Cellular Control ◽  
Muscle Triglyceride

The cellular control of intramuscular triglyceride (TG) metabolism involves two major identified lipases: hormone-sensitive lipase (HSL) and lipoprotein lipase (LPL). Recently, the presence of HSL in muscle has been unequivocally demonstrated. However, although it is thought that HSL is responsible for intramuscular TG lipolysis, direct evidence for this is lacking. There is evidence to suggest that HSL and LPL are simultaneously activated under a variety of conditions. The two muscle lipases appear to be turned on by the same signal and function as a coordinated unit in meeting the energy demands of muscle. At a time when HSL is presumably hydrolyzing endogenous TG, LPL is sent to the capillary beds in search of substrate. TG uptake from circulation is highly related to muscle LPL activity. Exercise training increases LPL activity in plasma and in parenchymal cells in muscle. These results suggest that training may increase the capacity to clear TG from circulation and that LPL might have a role in replenishing muscle TG stores that have been decreased with exercise.

scholarly journals Adipose Triglyceride Lipase and Hormone-sensitive Lipase Are the Major Enzymes in Adipose Tissue Triacylglycerol Catabolism

2006 ◽  
Vol 281 (52) ◽  
pp. 40236-40241 ◽  
Author(s):  
Martina Schweiger ◽  
Renate Schreiber ◽  
Guenter Haemmerle ◽  
Achim Lass ◽  
Christian Fledelius ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Adipose Triglyceride Lipase ◽  
Triglyceride Lipase ◽  
Hormone Sensitive

scholarly journals Effect of Bariatric Weight Loss on the Adipose Lipolytic Transcriptome in Obese Humans

2015 ◽  
Vol 2015 ◽  
pp. 1-7 ◽  
Author(s):  
Shakun Karki ◽  
Melissa G. Farb ◽  
Samantha Myers ◽  
Caroline Apovian ◽  
Donald T. Hess ◽  
...  
Keyword(s):  
Weight Loss ◽  
Adipose Tissue ◽  
Disease Risk ◽  
Cardiovascular Disease Risk ◽  
Human Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Triglyceride Hydrolysis ◽  
Hormone Sensitive ◽  
Obese Subjects ◽  
Subcutaneous Adipose

Background.Dysregulated lipolysis has been implicated in mechanisms of cardiometabolic disease and inflammation in obesity.Purpose. We sought to examine the effect of bariatric weight loss on adipose tissue lipolytic gene expression and their relationship to systemic metabolic parameters in obese subjects.Methods/Results. We biopsied subcutaneous adipose tissue in 19 obese individuals (BMI 42 ± 5 kg/m2, 79% female) at baseline and after a mean period of 8 ± 5 months (range 3–15 months) following bariatric surgery. We performed adipose tissue mRNA expression of proteins involved in triglyceride hydrolysis and correlated their weight loss induced alterations with systemic parameters associated with cardiovascular disease risk. mRNA transcripts of adipose triglyceride lipase (ATGL), hormone-sensitive lipase (HSL), and lipid droplet proteins comparative gene identification 58 (CGI-58) and perilipin increased significantly after weight loss (p<0.05for all). ATGL expression correlated inversely with plasma triglyceride (TG), hemoglobin A1C (HbA1C), and glucose, and HSL expression correlated negatively with glucose, while CGI-58 was inversely associated with HbA1C.Conclusion. We observed increased expression of adipose tissue lipolytic genes following bariatric weight loss which correlated inversely with systemic markers of lipid and glucose metabolism. Functional alterations in lipolysis in human adipose tissue may play a role in shaping cardiometabolic phenotypes in human obesity.

scholarly journals Hormone-sensitive lipase of rat adipose tissue. Purification and some properties.

1981 ◽  
Vol 256 (12) ◽  
pp. 6311-6320
Author(s):  
G. Fredrikson ◽  
P. Strålfors ◽  
N.O. Nilsson ◽  
P. Belfrage
Keyword(s):  
Adipose Tissue ◽  
Hormone Sensitive Lipase ◽  
Hormone Sensitive ◽  
Rat Adipose Tissue

scholarly journals Effects of Physiological Hypercortisolemia on the Regulation of Lipolysis in Subcutaneous Adipose Tissue1

1998 ◽  
Vol 83 (2) ◽  
pp. 626-631 ◽  
Author(s):  
Jaswinder S. Samra ◽  
Mo L. Clark ◽  
Sandy M. Humphreys ◽  
Ian A. MacDonald ◽  
Peter A. Bannister ◽  
...  
Keyword(s):  
Adipose Tissue ◽  
Lipoprotein Lipase ◽  
Sodium Succinate ◽  
Hormone Sensitive Lipase ◽  
Whole Body ◽  
Constant Infusion ◽  
Nonesterified Fatty Acid ◽  
Significant Difference ◽  
Hormone Sensitive ◽  
Subcutaneous Adipose

Cortisol is known to increase whole body lipolysis, yet chronic hypercortisolemia results in increased fat mass. The main aim of the study was to explain these two apparently opposed observations by examining the acute effects of hypercortisolemia on lipolysis in subcutaneous adipose tissue and in the whole body. Six healthy subjects were studied on two occasions. On one occasion hydrocortisone sodium succinate was infused iv to induce hypercortisolemia (mean plasma cortisol concentrations, 1500 ± 100 vs. 335± 25 nmol/L; P &lt; 0.001); on the other occasion (control study) no intervention was made. Lipolysis in the sc adipose tissue of the anterior abdominal wall was studied by measurement of arterio-venous differences, and lipolysis in the whole body was studied by constant infusion of[ 1,2,3-2H5]glycerol for measurement of the systemic glycerol appearance rate. Hypercortisolemia led to significantly increased arterialized plasma nonesterified fatty acid (NEFA; P &lt; 0.01) and blood glycerol concentrations (P &lt; 0.05), with an increase in systemic glycerol appearance (P &lt; 0.05). However, in sc abdominal adipose tissue, hypercortisolemia decreased veno-arterialized differences for NEFA (P &lt; 0.05) and reduced NEFA efflux (P &lt; 0.05). This reduction was attributable to decreased intracellular lipolysis (P &lt; 0.05), reflecting decreased hormone-sensitive lipase action in this adipose depot. Hypercortisolemia caused a reduction in arterialized plasma TAG concentrations (P &lt; 0.05), but without a significant change in the local extraction of TAG (presumed to reflect the action of adipose tissue lipoprotein lipase). There was no significant difference in plasma insulin concentrations between the control and hypercortisolemia study. Site-specific regulation of the enzymes of intracellular lipolysis (hormone-sensitive lipase) and intravascular lipolysis (lipoprotein lipase) may explain the ability of acute cortisol treatment to increase systemic glycerol and NEFA appearance rates while chronically promoting net central fat deposition.

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