A nanomaterial-free and thionine labeling-based lateral flow immunoassay for rapid and visual detection of the transgenic CP4-EPSPS protein

2022 ◽  
pp. 132112
Author(s):  
Jinbin Wang ◽  
Qianwen Yang ◽  
Hua Liu ◽  
Yifan Chen ◽  
Wei Jiang ◽  
...  
Keyword(s):  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Cp4 Epsps

scholarly journals Magnetized Carbon Nanotube Based Lateral Flow Immunoassay for Visual Detection of Complement Factor B

Molecules ◽  
2019 ◽  
Vol 24 (15) ◽  
pp. 2759 ◽  
Author(s):  
Yan Huang ◽  
Tingting Wu ◽  
Fang Wang ◽  
Kun Li ◽  
Lisheng Qian ◽  
...  
Keyword(s):  
Carbon Nanotube ◽  
Human Blood ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Complement Factor ◽  
Factor B ◽  
Complement Factor B ◽  
Flow Test ◽  
Lateral Flow Test

The authors describe a magnetized carbon nanotube (MCNT) based lateral flow immunoassay (LFI) for visual detection of complement factor B (CFB) in blood. MCNT was prepared by decorating magnetic Fe3O4 nanoparticles on multi-walled CNT surface and used as a colored tag for LFI. Monoclonal antibody (mAb, Ab1) of CFB was covalently immobilized on the MCNT surface via diimide-activated conjugation between the carboxyl groups on the MCNT surface and amino groups of antibodies. Polyclonal antibody of CFB (Ab2) and the secondary antibody were used to prepare the lateral flow test strips. The assay involved: (1) the capture of CFB in blood with the mAb-functionalized MCNT; (2) magnetic separation of the formed CFB-mAb-MCNT and excess of mAb-MCNT from the blood with an external magnet; (3) lateral flow test to capture the CFB-mAb-MCNT complex on the test zone and the excess of mAb-MCNT on the control zone; (4) Recording the intensities of the produced the characteristic brown bands with a portable strip reader and quantitating the concentration of CFB. The proof-of-concept was demonstrated by testing CFB in the buffer, and the detection limit was 5 ng mL−1 under the optimized analytical parameters. CFB in 1 μL of human blood was detected successfully in 30 min with this LFI and the results had a high correlation with commercial ELISA kit. Thence, the MCNT-based LFI offers a rapid and low-cost tool for detecting CFB in human blood directly.

scholarly journals Carbon nanotag based visual detection of influenza A virus by a lateral flow immunoassay

2017 ◽  
Vol 184 (6) ◽  
pp. 1827-1835 ◽  
Author(s):  
Natpapas Wiriyachaiporn ◽  
Hathainan Sirikett ◽  
Weerakanya Maneeprakorn ◽  
Tararaj Dharakul
Keyword(s):  
Influenza A Virus ◽  
Influenza A ◽  
Visual Detection ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay

An Aggregation-Induced Emission Material Labeling Antigen-Based Lateral Flow Immunoassay Strip for Rapid Detection of Escherichia coli O157:H7

2021 ◽  
pp. 247263032098193
Author(s):  
Cheng Liu ◽  
Shuiqin Fang ◽  
Yachen Tian ◽  
Youxue Wu ◽  
Meijiao Wu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Rapid Detection ◽  
Foodborne Pathogen ◽  
Test Strip ◽  
Lateral Flow ◽  
Detection Sensitivity ◽  
Lateral Flow Immunoassay ◽  
Escherichia Coli O157 ◽  
Aggregation Induced Emission ◽  
E Coli

Escherichia coli O157:H7 ( E. coli O157:H7) is a dangerous foodborne pathogen, mainly found in beef, milk, fruits, and their products, causing harm to human health or even death. Therefore, the detection of E. coli O157:H7 in food is particularly important. In this paper, we report a lateral flow immunoassay strip (LFIS) based on aggregation-induced emission (AIE) material labeling antigen as a fluorescent probe for the rapid detection of E. coli O157:H7. The detection sensitivity of the strip is 105 CFU/mL, which is 10 times higher than that of the colloidal gold test strip. This method has good specificity and stability and can be used to detect about 250 CFU of E. coli O157:H7 successfully in 25 g or 25 mL of beef, jelly, and milk. AIE-LFIS might be valuable in monitoring food pathogens for rapid detection.

Upconversion nanoparticles-based lateral flow immunoassay for point-of-care diagnosis of periodontitis

2021 ◽  
Vol 334 ◽  
pp. 129673
Author(s):  
Wanghong He ◽  
Minli You ◽  
Zedong Li ◽  
Lei Cao ◽  
Feng Xu ◽  
...  
Keyword(s):  
Point Of Care ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Upconversion Nanoparticles

scholarly journals Ten Years of Lateral Flow Immunoassay Technique Applications: Trends, Challenges and Future Perspectives

Sensors ◽  
2021 ◽  
Vol 21 (15) ◽  
pp. 5185
Author(s):  
Fabio Di Nardo ◽  
Matteo Chiarello ◽  
Simone Cavalera ◽  
Claudio Baggiani ◽  
Laura Anfossi
Keyword(s):  
Environmental Control ◽  
Improve Patient Care ◽  
Turnaround Time ◽  
Lateral Flow ◽  
Lateral Flow Immunoassay ◽  
Future Perspectives ◽  
Immunoassay Technique ◽  
Food And Feed ◽  
Application Fields ◽  
Feed Safety

The Lateral Flow Immunoassay (LFIA) is by far one of the most successful analytical platforms to perform the on-site detection of target substances. LFIA can be considered as a sort of lab-in-a-hand and, together with other point-of-need tests, has represented a paradigm shift from sample-to-lab to lab-to-sample aiming to improve decision making and turnaround time. The features of LFIAs made them a very attractive tool in clinical diagnostic where they can improve patient care by enabling more prompt diagnosis and treatment decisions. The rapidity, simplicity, relative cost-effectiveness, and the possibility to be used by nonskilled personnel contributed to the wide acceptance of LFIAs. As a consequence, from the detection of molecules, organisms, and (bio)markers for clinical purposes, the LFIA application has been rapidly extended to other fields, including food and feed safety, veterinary medicine, environmental control, and many others. This review aims to provide readers with a 10-years overview of applications, outlining the trends for the main application fields and the relative compounded annual growth rates. Moreover, future perspectives and challenges are discussed.

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