Microemulsification of clove essential oil improves its in vitro and in vivo control of Penicillium digitatum

Food Control ◽  
2016 ◽  
Vol 65 ◽  
pp. 106-111 ◽  
Author(s):  
Shoukui He ◽  
Xiaoyun Ren ◽  
Yangfan Lu ◽  
Yunbin Zhang ◽  
Yifei Wang ◽  
...  
Keyword(s):  
Essential Oil ◽  
Penicillium Digitatum ◽  
Clove Essential Oil

scholarly journals Glycemic profile, inflammatory markers, biochemical and histopathological parameters of mice fed with fermented dairy beverage with clove essential oil

2021 ◽  
Vol 10 (4) ◽  
pp. e22110413826
Author(s):  
Keicy Sandy Silvestre de Souza ◽  
Anna Christina de Almeida ◽  
Stephanie Pedrosa de Oliveira ◽  
Igor Viana Brandi ◽  
Sérgio Henrique Sousa Santos ◽  
...  
Keyword(s):  
Essential Oil ◽  
Biochemical Parameters ◽  
Experimental Period ◽  
Potassium Sorbate ◽  
Histopathological Analysis ◽  
Clove Essential Oil ◽  
Anti Inflammatory ◽  
Fermented Dairy

Previous studies have shown that the clove essential oil concentration of 200 mg/kg did not present toxicity and histopathological changes in the liver. Based on the evidence presented in this  study, we decided to evaluate in vivo the ability of a previously developed fermented dairy beverage, tested only in vitro, in which the standard chemical preservative, potassium sorbate, was replaced by clove essential oil (2 μl/ml), to be a functional food. To this end, twenty-four male Swiss mice were divided into three groups and submitted insulin sensitivity (IST) and glucose tolerance (GTT) testing, evaluation of physiological and biochemical parameters, histopathological analysis and quantification of the expression of anti-inflammatory and pro-inflammatory cytokines in the liver, after administering daily of the fermented dairy beverage with or without clove essential oil by gavage during 30 days. The group that received the fermented dairy beverage with clove essential oil showed a lower glycemic level in GTT, a higher sensitivity to insulin and a higher glucose decay constant rate (Kitt), with relation to the other groups. In the evaluation of physiological parameters there were no signs of toxicity in the mice during the experimental period. The biochemical parameters, histopathological analysis and expression of anti-inflammatory (IL-10) and pro-inflammatory (IL1β, IL6 e TNFα) cytokines in the liver of the mice, was not significantly affected by the treatment. These results corroborated by bioinformatics analysis demonstrate that the fermented dairy beverage with clove essential oil it can function as a substitute for conventional chemical preservatives and reduce glycemic levels.

scholarly journals Alternative control of post-harvest diseases in Tainung 1 papaya

2018 ◽  
Vol 48 (1) ◽  
pp. 29-35 ◽  
Author(s):  
Andréa Mirne de Macêdo Dantas ◽  
Selma Rogéria de Carvalho Nascimento ◽  
Beatriz Letícia Silva da Cruz ◽  
Fernando Henrique Alves da Silva ◽  
Márcia Michelle de Queiroz Ambrósio ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Vitro Experiment ◽  
In Vitro Experiment ◽  
Post Harvest ◽  
Randomized Design ◽  
Clove Essential Oil

ABSTRACT Controlling post-harvest papaya diseases without using agrochemicals is a challenge for producers. This study aimed at evaluating the effect of clove essential oil, biological fungicide (Trichodermil®), resistance inducer (Cob Sistem®) and chemical fungicide (Imazacure®) on the in vitro control of phytopathogenic fungi isolates from papaya as well as on the post-harvest quality of Tainung 1 papaya. The in vitro experiment was conducted in a complete randomized design, with five fungal species x five treatments and five replications. The in vivo experiment was conducted in a complete randomized design, with five treatments x five storage times, five replications and three fruits per replication. The fruits were stored under refrigeration at 10 ± 2 ºC and 90 ± 5 % of relative humidity and evaluated at 0, 7, 14, 21 and 28 days of storage, plus two shelf life days at 25 ± 2 ºC, to simulate marketing conditions. The inhibition of mycelial growth was evaluated in the in vitro experiment, while the diseases occurrence and post-harvest quality of the fruits were evaluated in the in vivo experiment. The clove essential oil and Trichodermil® were as efficient as Imazacure® in inhibiting the mycelial growth of Alternaria sp., Colletotrichum gloeosporioides and Rhizopus sp. The treatments with clove essential oil, Trichodermil® and Imazacure® were similar in controlling the pathogens up to 21 days of storage. The treatments had no effect on the fruits soluble solid contents.

scholarly journals Control of Some Phytopathogenic Fungi Using Clove Essential Oil (Syzygium aromaticum L.)

2019 ◽  
pp. 1-11
Author(s):  
Tiago Silva Lima ◽  
Kevison Romulo da Silva França ◽  
Plinio Tércio Medeiros de Azevedo ◽  
Yaroslávia Ferreira Paiva ◽  
José Carlos Santos Silva ◽  
...  
Keyword(s):  
Essential Oil ◽  
Mycelial Growth ◽  
Syzygium Aromaticum ◽  
In Vitro Test ◽  
In Vivo Test ◽  
Maize Seeds ◽  
Vitro Test ◽  
Clove Essential Oil

Aims: This study evaluates the inhibitory potential of the clove essential oil (Syzygium aromaticum L.) on phytopathogenic fungi in vitro and on maize seeds. Study Design: The experiments comprised completely randomized designs: Seven treatments with five replicates on in vitro test; and four treatments with five replicates each, on in vivo test. Place and Duration of Study: The work was carried out at the Center for Agrifood Science and Technology of the Federal University of Campina Grande, Pombal, Brazil, from April to May 2018. Methodology: In the in vitro test, the essential oil was incorporated into the PDA (Potato-Dextrose-Agar) culture medium. The treatments comprised five concentrations of the oil (0.0125, 0.025, 0.05, 0.1, and 0.2%), a negative control (0.0%), and a positive control (Tiram). Plates were inoculated with the tested fungi, Fusarium verticillioides, Macrophomina phaseolina, and Macrophomina pseudophaseolina, then incubated for seven days at 27±2°C. The percentage of mycelial growth inhibition (PGI) and mycelial growth rate index (MGRI) were estimated. In the in vivo test, maize seeds (AG1051 hybrid) were treated with the essential oil on concentrations equal or superior to the minimum inhibitory concentration found in the in vitro test, besides the negative and positive controls. The artificial inoculation was carried out in fungi colonies for 32 hours and the seed sanity test was performed. The percentage of seeds infected by the fungus was evaluated after seven days. Results: In vitro conditions, clove oil totally inhibited the mycelial growth of F. verticillioides, M. phaseolina and M. pseudophaseolina at concentrations of 0.05, 0.1 and 0.1%, respectively. At 0.2% concentration significantly reduced the incidence of colonies of fungi M. phaseolina and M. pseudophaseolina in hybrid corn seeds AG 1051. Conclusion: The clove essential oil had a fungitoxic effect on the phytopathogens evaluated, under in vitro and in the treatment of maize seeds.

The in vivo and in vitro angiogenic evaluation of the essential oil of Echinophora tournefortii

Planta Medica ◽  
2010 ◽  
Vol 76 (12) ◽  
Author(s):  
B Demirci ◽  
T Kiyan ◽  
A Koparal ◽  
M Kaya ◽  
F Demirci ◽  
...  
Keyword(s):  
Essential Oil

scholarly journals Amelioration of lipid peroxidation in vivo and in vitro by Satureja khozestanica essential oil in alloxan-induced diabetic rats

2014 ◽  
Vol 13 (1) ◽  
Author(s):  
Hassan Ahmadvand ◽  
Majid Tavafi ◽  
Ali Khosrowbeygi ◽  
Gholamreza Shahsavari ◽  
Maryam Hormozi ◽  
...  
Keyword(s):  
Lipid Peroxidation ◽  
Essential Oil ◽  
Diabetic Rats

Neurotrophic Properties of Silexan, an Essential Oil from the Flowers of Lavender-Preclinical Evidence for Antidepressant-Like Properties

2020 ◽  
Vol 54 (01) ◽  
pp. 37-46
Author(s):  
Kristina Friedland ◽  
Giacomo Silani ◽  
Anita Schuwald ◽  
Carola Stockburger ◽  
Egon Koch ◽  
...  
Keyword(s):  
Essential Oil ◽  
Hippocampal Neurons ◽  
Day Treatment ◽  
Neuronal Cell ◽  
Antidepressant Activity ◽  
In Vivo Models ◽  
Antidepressant Effects ◽  
Primary Hippocampal Neurons

Abstract Background Silexan, a special essential oil from flowering tops of lavandula angustifolia, is used to treat subsyndromal anxiety disorders. In a recent clinical trial, Silexan also showed antidepressant effects in patients suffering from mixed anxiety-depression (ICD-10 F41.2). Since preclinical data explaining antidepressant properties of Silexan are missing, we decided to investigate if Silexan also shows antidepressant-like effects in vitro as well as in vivo models. Methods We used the forced swimming test (FST) in rats as a simple behavioral test indicative of antidepressant activity in vivo. As environmental events and other risk factors contribute to depression through converging molecular and cellular mechanisms that disrupt neuronal function and morphology—resulting in dysfunction of the circuitry that is essential for mood regulation and cognitive function—we investigated the neurotrophic properties of Silexan in neuronal cell lines and primary hippocampal neurons. Results The antidepressant activity of Silexan (30 mg/kg BW) in the FST was comparable to the tricyclic antidepressant imipramine (20 mg/kg BW) after 9-day treatment. Silexan triggered neurite outgrowth and synaptogenesis in 2 different neuronal cell models and led to a significant increase in synaptogenesis in primary hippocampal neurons. Silexan led to a significant phosphorylation of protein kinase A and subsequent CREB phosphorylation. Conclusion Taken together, Silexan demonstrates antidepressant-like effects in cellular as well as animal models for antidepressant activity. Therefore, our data provides preclinical evidence for the clinical antidepressant effects of Silexan in patients with mixed depression and anxiety.

scholarly journals Antitumor Effect of the Essential Oil from the Leaves of Croton matourensis Aubl. (Euphorbiaceae)

Molecules ◽  
2018 ◽  
Vol 23 (11) ◽  
pp. 2974 ◽  
Author(s):  
Emilly Lima ◽  
Rafaela Alves ◽  
Gigliola D´Elia ◽  
Talita Anunciação ◽  
Valdenizia Silva ◽  
...  
Keyword(s):  
Gas Chromatography ◽  
Essential Oil ◽  
Cancer Cell ◽  
Hepg2 Cells ◽  
Membrane Integrity ◽  
In Vitro Cytotoxicity ◽  
Cell Cycle Distribution ◽  
Antitumor Effects

Croton matourensis Aubl. (synonym Croton lanjouwensis Jabl.), popularly known as “orelha de burro”, “maravuvuia”, and/or “sangrad’água”, is a medicinal plant used in Brazilian folk medicine as a depurative and in the treatment of infections, fractures, and colds. In this work, we investigated the chemical composition and in vitro cytotoxic and in vivo antitumor effects of the essential oil (EO) from the leaves of C. matourensis collected from the Amazon rainforest. The EO was obtained by hydrodistillation using a Clevenger-type apparatus and characterized qualitatively and quantitatively by gas chromatography coupled to mass spectrometry (GC–MS) and gas chromatography with flame ionization detection (GC–FID), respectively. In vitro cytotoxicity of the EO was assessed in cancer cell lines (MCF-7, HCT116, HepG2, and HL-60) and the non-cancer cell line (MRC-5) using the Alamar blue assay. Furthermore, annexin V-FITC/PI staining and the cell cycle distribution were evaluated with EO-treated HepG2 cells by flow cytometry. In vivo efficacy of the EO (40 and 80 mg/kg/day) was demonstrated in C.B-17 severe combined immunodeficient (SCID) mice with HepG2 cell xenografts. The EO included β-caryophyllene, thunbergol, cembrene, p-cymene, and β-elemene as major constituents. The EO exhibited promising cytotoxicity and was able to cause phosphatidylserine externalization and DNA fragmentation without loss of the cell membrane integrity in HepG2 cells. In vivo tumor mass inhibition rates of the EO were 34.6% to 55.9%. Altogether, these data indicate the anticancer potential effect of C. matourensis.

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