Phylogenetic and toxinogenic characteristics of Bacillus cereus group members isolated from spices and herbs

Food Control ◽  
2018 ◽  
Vol 83 ◽  
pp. 90-98 ◽  
Author(s):  
Hendrik Frentzel ◽  
Britta Kraushaar ◽  
Gladys Krause ◽  
Dorina Bodi ◽  
Heidi Wichmann-Schauer ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Cereus Group ◽  
Group Members

scholarly journals Prevalence, Genetic Diversity, and Host Range of Tectiviruses among Members of the Bacillus cereus Group

2014 ◽  
Vol 80 (14) ◽  
pp. 4138-4152 ◽  
Author(s):  
Annika Gillis ◽  
Jacques Mahillon
Keyword(s):  
Genetic Diversity ◽  
Bacillus Cereus ◽  
Host Range ◽  
Simple Relationship ◽  
Biological Processes ◽  
Bacillus Cereus Group ◽  
Variable Regions ◽  
Content Type ◽  
The Family ◽  
Group Members

ABSTRACTGIL01, Bam35, GIL16, AP50, and Wip1 are tectiviruses preying on theBacillus cereusgroup. Despite the significant contributions of phages in different biological processes, little is known about the dealings taking place between tectiviruses and their Gram-positive bacterial hosts. Therefore, this work focuses on characterizing the interactions between tectiviruses and theB. cereusgroup by assessing their occurrence and genetic diversity and evaluating their host range. To study the occurrence of tectiviruses in theB. cereusgroup, 2,000 isolates were evaluated using primers designed to be specific to two variable regions detected in previously described elements. PCR and propagation tests revealed that tectivirus-like elements occurred in less than 3% of the isolates. Regardless of this limited distribution, several novel tectiviruses were found, and partial DNA sequencing indicated that a greater diversity exists within the familyTectiviridae. Analyses of the selected variable regions, along with their host range, showed that tectiviruses in theB. cereusgroup can be clustered mainly into two different groups: the ones infectingB. anthracisand those isolated from otherB. cereusgroup members. In order to address the host range of some novel tectiviruses, 120 strains were tested for sensitivity. The results showed that all the tested tectiviruses produced lysis in at least oneB. cereus sensu latostrain. Moreover, no simple relationship between the infection patterns of the tectiviruses and their diversity was found.

scholarly journals Discrimination of the Bacillus cereus Group Members by Pattern Analysis of Random Amplified Polymorphic DNA-PCR

2012 ◽  
Vol 17 (2) ◽  
pp. 83-86 ◽  
Author(s):  
RITSUKO KUWANA ◽  
DAISUKE IMAMURA ◽  
HIROMU TAKAMATSU ◽  
KAZUHITO WATABE
Keyword(s):  
Bacillus Cereus ◽  
Pattern Analysis ◽  
Random Amplified Polymorphic Dna ◽  
Bacillus Cereus Group ◽  
Group Members ◽  
Dna Pcr

Germination and outgrowth of spores of Bacillus cereus group members: Diversity and role of germinant receptors

2011 ◽  
Vol 28 (2) ◽  
pp. 199-208 ◽  
Author(s):  
Tjakko Abee ◽  
Masja Nierop Groot ◽  
Marcel Tempelaars ◽  
Marcel Zwietering ◽  
Roy Moezelaar ◽  
...  
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Cereus Group ◽  
Group Members

scholarly journals Cell Wall Carbohydrate Compositions of Strains from the Bacillus cereus Group of Species Correlate with Phylogenetic Relatedness

2007 ◽  
Vol 190 (1) ◽  
pp. 112-121 ◽  
Author(s):  
Christine Leoff ◽  
Elke Saile ◽  
David Sue ◽  
Patricia Wilkins ◽  
Conrad P. Quinn ◽  
...  
Keyword(s):  
Cell Wall ◽  
Bacillus Cereus ◽  
Cell Walls ◽  
Regulatory Gene ◽  
Severe Pneumonia ◽  
Dependent Manner ◽  
Bacillus Cereus Group ◽  
Group Members ◽  
Cell Wall Carbohydrates ◽  
Clade 1

ABSTRACT Members of the Bacillus cereus group contain cell wall carbohydrates that vary in their glycosyl compositions. Recent multilocus sequence typing (MLST) refined the relatedness of B. cereus group members by separating them into clades and lineages. Based on MLST, we selected several B. anthracis, B. cereus, and B. thuringiensis strains and compared their cell wall carbohydrates. The cell walls of different B. anthracis strains (clade 1/Anthracis) were composed of glucose (Glc), galactose (Gal), N-acetyl mannosamine (ManNAc), and N-acetylglucosamine (GlcNAc). In contrast, the cell walls from clade 2 strains (B. cereus type strain ATCC 14579 and B. thuringiensis strains) lacked Gal and contained N-acetylgalactosamine (GalNAc). The B. cereus clade 1 strains had cell walls that were similar in composition to B. anthracis in that they all contained Gal. However, the cell walls from some clade 1 strains also contained GalNAc, which was not present in B. anthracis cell walls. Three recently identified clade 1 strains of B. cereus that caused severe pneumonia, i.e., strains 03BB102, 03BB87, and G9241, had cell wall compositions that closely resembled those of the B. anthracis strains. It was also observed that B. anthracis strains cell wall glycosyl compositions differed from one another in a plasmid-dependent manner. When plasmid pXO2 was absent, the ManNAc/Gal ratio decreased, while the Glc/Gal ratio increased. Also, deletion of atxA, a global regulatory gene, from a pXO2− strain resulted in cell walls with an even greater level of Glc.

Phenotypic properties and genotyping analysis of Bacillus cereus group isolates from dairy and potato products

LWT ◽  
2021 ◽  
Vol 140 ◽  
pp. 110853
Author(s):  
Yiying Huang ◽  
Steve H. Flint ◽  
Shubo Yu ◽  
Yu Ding ◽  
Jon S. Palmer
Keyword(s):  
Bacillus Cereus ◽  
Bacillus Cereus Group ◽  
Phenotypic Properties ◽  
Potato Products

Development of Rapid Real-Time PCR and Most-Probable-Number Real-Time PCR Assays To Quantify Enterotoxigenic Strains of the Species in the Bacillus cereus Group

2007 ◽  
Vol 70 (12) ◽  
pp. 2774-2781 ◽  
Author(s):  
I-CHEN YANG ◽  
DANIEL YANG-CHIH SHIH ◽  
JAN-YI WANG ◽  
TZU-MING PAN
Keyword(s):  
Bacillus Cereus ◽  
Real Time ◽  
Real Time Pcr ◽  
Food Samples ◽  
Most Probable Number ◽  
Pcr Assay ◽  
Bacillus Cereus Group ◽  
Probable Number ◽  
Cooked Rice ◽  
Group Detection

Members of the Bacillus cereus group may produce diarrheal enterotoxins and could be potential hazards if they enter the food chain. Therefore, a method capable of detecting all the species in the B. cereus group rather than B. cereus alone is important. We selected nhe as the target and developed a real-time PCR assay to quantify enterotoxigenic strains of the B. cereus group. The real-time PCR assay was evaluated with 60 B. cereus group strains and 28 others. The assay was also used to construct calibration curves for different food matrices and feces. The assay has an excellent quantification capacity, as proved by its linearity (R2 > 0.993), wide dynamic quantification range (102 to 107 CFU/g for cooked rice and chicken, 103 to 107 CFU/ml for milk, and 104 to 107 CFU/g for feces), and adequate relative accuracy (85.5 to 101.1%). For the low-level contaminations, a most-probable-number real-time PCR assay was developed that could detect as low as 100 CFU/ml. Both assays were tested with real food samples and shown to be considerably appropriate for B. cereus group detection and quantification.

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