Over 5 years, macrofungi from six habitats in Clayoquot Sound, Vancouver Island, British Columbia, were documented. Habitats were categorized as dune, spruce fringe, old-growth rainforest, second-growth forest, bog, or estuarine. All but the second-growth forest are natural ecosystems. A total of 551 taxa of macrofungi were recorded. Between 17% and 36% of the species in any one habitat were found only in that habitat. The most frequently encountered and ubiquitous species was Craterellus tubaeformis (Fr.) Quel., found in all years, habitats, and sites. Of the 551 taxa, only 28 were found every year, and 308 were found in only 1 year. Rare species that were recorded include Cordyceps ravenelii Berkeley & Curtis, Hygrophorus inocybiformis Smith, and Tricholoma apium Schaeffer in the dunes and Stereopsis humphreyi (Burt) Redhead in the spruce fringe. Similarities between habitats based on taxa in common showed that bog and estuarine habitats had only 9%17% in common with each other and the other habitats, whereas dune, spruce fringe, and the two forest types shared 21%31% of their species. Old-growth rainforest yielded approximately 4 times as many species as bog and estuarine habitats, and approximately 1.5 times as many as the other three habitats.Key words: Clayoquot Sound, Vancouver Island, macrofungi, habitats, biodiversity.