<p>Woody plants are known to emit methane (CH<sub>4</sub>) as an important greenhouse gas into the atmosphere. Recent studies show that tree stems might be also sinks for CH<sub>4</sub>; however, the mechanisms of CH<sub>4</sub> uptake and its fate are unknown. Norway spruce (<em>Picea abies)</em> is characterised as negligible CH<sub>4 </sub>source in boreal forests. Even though spruce trees have been widely planted for its wood in large-scale monocultures in European temperate forests, no studies have focused on their CH<sub>4</sub> exchange potential in the temperate zone.</p><p>We determined stems of Norway spruce growing in a temperate zone aiming to find out whether the tree stems exchange CH<sub>4</sub> with the atmosphere and how they contribute to the forest trace gas exchange.</p><p>The measurements were performed at the experimental station of the &#8216;Kranzberg Forest Roof Experiment&#8217; near Freising, Germany, in June 2019. Fluxes of CH<sub>4</sub> in mature tree stems were measured using non-steady-state stem chamber systems (n=32) installed in stem vertical profile approx. two weeks prior to measurements using a portable greenhouse gas analyser. Moreover, resins sampled from spruce stems were investigated for their CH<sub>4</sub> exchange potential. Control measurements were performed to ensure that the fluxes do not originate from used chamber materials, in particular silicones used for chamber installation.</p><p>Our preliminary results show that the spruce stems can be a strong sink for CH<sub>4</sub> (-0.288 &#177; 0.053 mg CH<sub>4</sub> m<sup>-2</sup> stem area h<sup>-1</sup>, mean &#177; s.e.), even if a small amount of resin is present on the bark. The stems exuded resins to different extent (covering 4.8 &#177; 1.3% of the stem surface area in chambers), partly as a result of smoothening of rough surface layers of dead bark for chamber installation. However, even spruce stems without obvious &#8220;injuries&#8221; released small amounts of resins for unknown reasons (response to drought, bark-beetle attack, etc.?). The incubated resin samples consistently consumed CH<sub>4</sub> (-12.0 &#177; 1.7 mg CH<sub>4</sub> m<sup>-2</sup> resin area h<sup>-1</sup>). Moreover, the detected stem CH<sub>4</sub> uptake negatively correlated with the resin occurrence in the stem chambers (R&#178; = 0.884). After re-calculation of the stem fluxes to resin area, the CH<sub>4</sub> consumption rates of stems and resin samples were in the same order of magnitude at median level (-13.2 and -12.0 mg CH<sub>4</sub> m<sup>-2</sup> resin area h<sup>-1</sup>, resp.).</p><p>Concluded, the spruce resins appear to be a very strong and until now undiscovered sink for CH<sub>4</sub>. Even one small droplet of resins on bark can turn the known negligible CH<sub>4</sub> exchange of intact spruce stems into strong CH<sub>4</sub> sinks, having thus severe impact on the overall forest CH<sub>4</sub> balance. This consumption potential of fresh resins should be considered by estimation of forest ecosystem CH<sub>4</sub> balance especially in areas, where resin bleeding is widely spread or is to be expected (bark-beetle areas, drought events, tree harvest, clear-cutting).</p><p>&#160;</p><p><em>Acknowledgement</em></p><p><em>This research was supported by the Czech Science Foundation (17-18112Y) and National Sustainability Program I (LO1415). We thank Prof. Thorsten Grams for all his kind support, and Jan Hrdli&#269;ka and Thomas Feuerbach for their technical support.</em></p><p>&#160;</p><p>&#160;</p>