Long-term melatonin or 17β-estradiol supplementation alleviates oxidative stress in ovariectomized adult rats

Early life exposure to immunological challenge has programming effects on the adult hypothalamo-pituitary-adrenocortical axis stress responsivity, and stress is known to suppress GnRH pulse generator activity, especially LH pulses. We investigated the effects of neonatal exposure to endotoxin on stress-induced suppression of pulsatile LH secretion and the involvement of corticotropin-releasing factor (CRF) receptor mechanisms in adult rats. Pups at 3 and 5 d of age were administered lipopolysaccharide (LPS, 50 μg/kg, ip). At 12 wk of age, they were ovariectomized and implanted with sc 17β-estradiol capsules and iv cannulas. Blood samples (25 μl) were collected every 5 min for 5 h for LH measurement. After 2 h of sampling, rats were given LPS (25 μg/kg, iv). CRF and CRF-R1 and CRF-R2 receptor mRNA was determined by RT-PCR in medial preoptic area (mPOA) micropunches collected at 3 h after LPS administration. There was no difference in basal LH pulse frequency between neonatal LPS- and neonatal saline-treated controls. However, neonatal endotoxin-treated rats exhibited a significantly greater LPS stress-induced suppression of LH pulse frequency. Basal mPOA CRF-R1 expression was unchanged in neonatal LPS- and neonatal saline-treated rats. However, CRF-R1 expression was significantly increased in response to LPS stress in neonatal LPS-treated animals but not in neonatal saline-treated controls. CRF and CRF-R2 expression was unchanged in all treatment groups. These data demonstrate that exposure to bacterial endotoxin in early neonatal life programs long-term sensitization of the GnRH pulse generator to the inhibitory influence of stress in adulthood, an effect that might involve up-regulation of CRF-R1 expression in the mPOA.

Download Full-text

Gold nanoparticles alter parameters of oxidative stress and energy metabolism in organs of adult rats

Biochemistry and Cell Biology ◽

10.1139/bcb-2015-0030 ◽

2015 ◽

Vol 93 (6) ◽

pp. 548-557 ◽

Cited By ~ 21

Author(s):

Gabriela Kozuchovski Ferreira ◽

Eria Cardoso ◽

Francieli Silva Vuolo ◽

Monique Michels ◽

Elton Torres Zanoni ◽

...

Keyword(s):

Oxidative Stress ◽

Gold Nanoparticles ◽

Energy Metabolism ◽

Acute Administration ◽

Sod Activity ◽

Adult Rats ◽

Protein Levels ◽

Carbonyl Protein ◽

Term Administration

This study evaluated the parameters of oxidative stress and energy metabolism after the acute and long-term administration of gold nanoparticles (GNPs, 10 and 30 nm in diameter) in different organs of rats. Adult male Wistar rats received a single intraperitoneal injection or repeated injections (once daily for 28 days) of saline solution, GNPs-10 or GNPs-30. Twenty-four hours after the last administration, the animals were killed, and the liver, kidney, and heart were isolated for biochemical analysis. We demonstrated that acute administration of GNPs-30 increased the TBARS levels, and that GNPs-10 increased the carbonyl protein levels. The long-term administration of GNPs-10 increased the TBARS levels, and the carbonyl protein levels were increased by GNPs-30. Acute administration of GNPs-10 and GNPs-30 increased SOD activity. Long-term administration of GNPs-30 increased SOD activity. Acute administration of GNPs-10 decreased the activity of CAT, whereas long-term administration of GNP-10 and GNP-30 altered CAT activity randomly. Our results also demonstrated that acute GNPs-30 administration decreased energy metabolism, especially in the liver and heart. Long-term GNPs-10 administration increased energy metabolism in the liver and decreased energy metabolism in the kidney and heart, whereas long-term GNPs-30 administration increased energy metabolism in the heart. The results of our study are consistent with other studies conducted in our research group and reinforce the fact that GNPs can lead to oxidative damage, which is responsible for DNA damage and alterations in energy metabolism.

Download Full-text

Lead-Induced Motor Dysfunction Is Associated with Oxidative Stress, Proteome Modulation, and Neurodegeneration in Motor Cortex of Rats

Oxidative Medicine and Cellular Longevity ◽

10.1155/2021/5595047 ◽

2021 ◽

Vol 2021 ◽

pp. 1-10

Author(s):

Luana Ketlen Reis Leão ◽

Leonardo Oliveira Bittencourt ◽

Ana Carolina Alves Oliveira ◽

Priscila Cunha Nascimento ◽

Maria Karolina Martins Ferreira ◽

...

Keyword(s):

Oxidative Stress ◽

Motor Cortex ◽

Wistar Rats ◽

Toxic Metal ◽

Bioinformatic Analysis ◽

Motor Dysfunction ◽

Motor Deficits ◽

Proteomic Profile ◽

Adult Rats

Lead (Pb) is a toxic metal with great neurotoxic potential. The aim of this study was to investigate the effects of a long-term Pb intoxication on the global proteomic profile, oxidative biochemistry and neuronal density in motor cortex of adult rats, and the possible outcomes related to motor functions. For this, Wistar rats received for 55 days a dose of 50 mg/Kg of Pb acetate by intragastric gavage. Then, the motor abilities were evaluated by open field and inclined plane tests. To investigate the possible oxidative biochemistry modulation, the levels of pro-oxidant parameters as lipid peroxidation and nitrites were evaluated. The global proteomic profile was evaluated by ultraefficiency liquid chromatography system coupled with mass spectrometry (UPLC/MS) followed by bioinformatic analysis. Moreover, it was evaluated the mature neuron density by anti-NeuN immunostaining. The statistical analysis was performed through Student’s t -test, considering p < 0.05 . We observed oxidative stress triggering by the increase in malonaldehyde and nitrite levels in motor cortex. In the proteomic analysis, the motor cortex presented alterations in proteins associated with neural functioning, morphological organization, and neurodegenerative features. In addition, it was observed a decrease in the number of mature neurons. These findings, associated with previous evidences observed in spinal cord, cerebellum, and hippocampus under the same Pb administration protocol, corroborate with the motor deficits in the rats towards Pb. Thus, we conclude that the long-term administration to Pb in young Wistar rats triggers impairments at several organizational levels, such as biochemical and morphological, which resulted in poor motor performance.

Download Full-text

Stress During the Pre-pubertal Period Leads to Long-Term Diet-Dependent Changes in Anxiety-Like Behavior and in Oxidative Stress Parameters in Male Adult Rats

Neurochemical Research ◽

10.1007/s11064-013-1083-3 ◽

2013 ◽

Vol 38 (9) ◽

pp. 1791-1800 ◽

Cited By ~ 7

Author(s):

Danusa Mar Arcego ◽

Rachel Krolow ◽

Carine Lampert ◽

Cristie Noschang ◽

Letícia Ferreira Pettenuzzo ◽

...

Keyword(s):

Oxidative Stress ◽

Adult Rats ◽

Male Adult ◽

Oxidative Stress Parameters ◽

Stress Parameters

Download Full-text

Effects of Catecholestrogens on Luteinizing Hormone Levels in Long Term Ovariectomized Adult Rats*

Endocrinology ◽

10.1210/endo-109-4-1037 ◽

1981 ◽

Vol 109 (4) ◽

pp. 1037-1039 ◽

Cited By ~ 13

Author(s):

PETER BALL ◽

GüNTER EMONS ◽

THOMAS KLINGEBIEL ◽

KATHRIN-MARIA GRüHN ◽

RUDOLF KNUPPEN

Keyword(s):

Luteinizing Hormone ◽

Adult Rats ◽

Hormone Levels ◽

Ovariectomized Adult Rats

Download Full-text

Hepatic expression of sodium-dependent vitamin C transporters: ontogeny, subtissular distribution and effect of chronic liver diseases

British Journal Of Nutrition ◽

10.1017/s0007114511002273 ◽

2011 ◽

Vol 106 (12) ◽

pp. 1814-1825 ◽

Cited By ~ 21

Author(s):

Rocio I. R. Macias ◽

Carlos Hierro ◽

Susana Cuesta de Juan ◽

Felipe Jimenez ◽

Francisco Gonzalez-San Martin ◽

...

Keyword(s):

Oxidative Stress ◽

Vitamin C ◽

Liver Diseases ◽

Chronic Liver ◽

Acid Uptake ◽

Biliary Cirrhosis ◽

Chronic Liver Diseases ◽

Adult Rats ◽

Hepatic Expression

Ascorbic acid uptake is a key step in determining the overall bioactivity of this vitamin. Expression of Na-dependent vitamin C transporters (SVCT; SLC23A1 and SLC23A2) during long-term oxidative stress occurring in several chronic liver diseases may determine the antioxidant defence in this organ. In patients with hepatocellular cholestasis, primary biliary cirrhosis, haemochromatosis and non-alcoholic steatohepatitis, using real-time RT-PCR, an enhanced hepatic expression of both SLC23A1 and SLC23A2, but not other organic anions transporters, such as OATP1A2, OATP1B1 and OATP1B3, was found. To further investigate these findings, we used secondary biliary cirrhosis induced in rats by long-term biliary obstruction as a model of chronic liver disease accompanied by oxidative stress because of bile acid accumulation. In control rat liver, expression of Slc23a1 was low at birth, increased progressively up to adulthood and decreased in senescence, whereas expression of Slc23a2 did not change significantly after birth. In 8-week-old rats, immunohistochemistry and confocal microscopy studies revealed the expression in hepatocytes and bile duct cells of mainly Slc23a1, whereas both Slc23a1 and Slc23a2 were expressed in endothelial, stellate and Kupffer cells. In adult rats, when obstructive cholestasis was maintained for 8 weeks, a significant up-regulation of Slc23a2 accompanied by a down-regulation of Slc23a1 was found. In sum, there is a selective cell-type distribution of SVCT in the liver tissue, which, in addition to differential control in the expression of both isoforms, may play a role in the ability of different liver cell types to take up vitamin C under physiological and pathological conditions.

Download Full-text