Purpose
– The purpose of this paper is to optimize production of cellulase enzyme from agricultural waste by using Aspergillus fumigatus JCF. The study also aims at the production of bioethanol using cellulase and yeast.
Design/methodology/approach
– Cellulase production was carried out using modified Mandel’s medium. The optimization of the cellulase production was carried out using Plackett-Burman and Response surface methodology. Bioethanol production was carried out using simultaneous saccharification and fermentation.
Findings
– Maximum cellulase production at optimized conditions was found to be 2.08 IU/ml. Cellulase was used for the saccharification of three different feed stocks, i.e. sugar cane leaves, corn cob and water hyacinth. Highest amount of reducing sugar was released was 29.1 gm/l from sugarcane leaves. Sugarcane leaves produced maximum bioethanol concentration of 9.43 g/l out of the three substrates studied for bioethanol production.
Originality/value
– The present study reveals that by using the agricultural wastes, cellulase production can be economically increased thereby bioethanol production.
The Effect of Substrate Loading On Simultaneous Saccharification And Fermentation Process For Bioethanol Production from Oil Palm Empty Fruit Bunches
