Evaluation of the matrix effect in the quantitative bio-oil analysis by gas chromatography

Background:Increased consumer demand for curry leaves free from pesticides demands fast and reliable analytical methods for the analysis of pesticide residues.Objective:The optimization of a QuEChERS based sample preparation technique with improved analytical accuracy by removing interfering matrix components for LC-MS/MS analysis of pesticide residues from curry leaves.Methods:A modified QuEChERS solid phase extraction method was developed and validated for the analysis of 26 pesticides in fresh and dried curry leaves. The effects of the sample preparation steps and column retention time on the matrix suppression of analyte ions were also evaluated.Results:Validation parameters were found within an acceptable range. The matrix effect evaluation studies showed that the QuEChERS sample preparation was able to minimize the ion suppression of analytes due to co-eluting matrix of components and that a d-SPE clean up step had major role in reducing matrix effect. The gradient mobile phase with longer retention time for analytes resulted in comparatively lesser matrix effects than the isocratic mobile phase of non-polar nature. Even after the clean up, a considerable number of compounds had more than 20% reduction in their MS response in the gradient mobile phase.Conclusion:This study emphasized the need of proper sample clean up before a LC-MS/MS analysis and the usage of matrix matched standards and mobile phase that ultimately results in an appropriate analyte separation in reasonable retention times.

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On the matrix-effect in the excitation of the atomic emission spectra of magnesites

Collection of Czechoslovak Chemical Communications ◽

10.1135/cccc19651303 ◽

1965 ◽

Vol 30 (4) ◽

pp. 1303-1310 ◽

Cited By ~ 1

Author(s):

M. Matherny ◽

N. Pliešovská ◽

Ž. Rybárová

Keyword(s):

Matrix Effect ◽

Emission Spectra ◽

Atomic Emission ◽

The Matrix

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Dilution of QuEChERS Extracts Without Cleanup Improves Results in the UHPLC-MS/MS Multiresidue Analysis of Pesticides in Tomato

Food Analytical Methods ◽

10.1007/s12161-020-01921-1 ◽

2021 ◽

Author(s):

Fabiane M. Stringhini ◽

Lucila C. Ribeiro ◽

Graziela I. Rocha ◽

Juliana D. de B. Kuntz ◽

Renato Zanella ◽

...

Keyword(s):

Matrix Effect ◽

Practical Method ◽

Analysis Method ◽

Multiresidue Analysis ◽

Limit Of Quantification ◽

Chromatography Tandem Mass Spectrometry ◽

The Matrix ◽

Liquid Chromatography Tandem Mass ◽

Ultrahigh Performance Liquid Chromatography ◽

Positive Results

AbstractTomato is well-known to be one of the most cultivated and consumed vegetables worldwide and frequently contain pesticide residues. Therefore, a simple multiresidue method was established and validated to determine 129 pesticides and metabolites in tomato samples using a modified acetate QuEChERS without cleanup for sample preparation and determination by ultrahigh-performance liquid chromatography tandem mass spectrometry (UHPLC-MS/MS). Dilution of the raw extract in different proportions of mobile phase was evaluated and a dilution of 10 times presented adequate results improving analysis performance while minimizing the matrix effect. Validation performed according to SANTE guideline presented satisfactory results. Practical method limit of quantification was 0.01 mg kg−1 for most compounds. Recoveries between 70 and 120% with precision ≤ 20% were found for most compounds and spike levels evaluated. Matrix effect results were not significant for most part of compounds. Method proved to be simple, robust, and effective to be applied in routine analysis. Method applicability was performed by analysis of samples commercialized in Brazil and positive results were found demonstrating the importance of the proposed method.

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