Molecular detection of Coccidioides spp. from environmental samples in Baja California: linking Valley Fever to soil and climate conditions

Abstract Coastal temperate waters undergo considerable intra- and interannual environmental variations, which is reflected in the dynamic nature of their zooplankton communities. Since zooplankton phenology is dependent on several factors, particularly temperature and spring bloom timing, it is imperative to understand how zooplankton communities may shift under future climate conditions with warmer temperatures and more variable spring bloom initiation. To examine zooplankton phenology and response to shifts in bloom timing, we analyzed fortnightly zooplankton and environmental samples collected in the northern Strait of Georgia (B.C., Canada), a large semi-enclosed temperate basin, in 2015 and 2016. Despite a 5-week difference in spring bloom timing, zooplankton community succession was remarkably similar between years. In both years, biomass peaked within the same calendar week and communities were separated into winter, early spring and summer-autumn assemblages that formed independent of the spring bloom timing. Although some species-level phenological differences were observed between years, predominately delayed population development, zooplankton communities appeared to demonstrate resilience to interannual environmental variations on the whole. If ongoing warming shifts the timing of zooplankton consumers’ life history timing, it could lead to a mismatch with their zooplankton prey resource that exhibits comparatively less interannual variability.

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Molecular detection of marine nematodes from environmental samples: overcoming eukaryotic interference

Aquatic Microbial Ecology ◽

10.3354/ame044097 ◽

2006 ◽

Vol 44 ◽

pp. 97-103 ◽

Cited By ~ 19

Author(s):

P Bhadury ◽

MC Austen ◽

DT Bilton ◽

PJD Lambshead ◽

AD Rogers ◽

...

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Marine Nematodes

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Molecular Detection of Viable Bacterial Pathogens in Water by Ratiometric Pre-rRNA Analysis

Applied and Environmental Microbiology ◽

10.1128/aem.01810-09 ◽

2009 ◽

Vol 76 (3) ◽

pp. 960-962 ◽

Cited By ~ 24

Author(s):

Gerard A. Cangelosi ◽

Kris M. Weigel ◽

Clarita Lefthand-Begay ◽

John S. Meschke

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Dna Detection ◽

Bacterial Pathogens ◽

Bacterial Cells ◽

Viability Test ◽

Stimulation Of

ABSTRACT Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.

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Molecular Detection of Northern Leatherside Chub (Lepidomeda copei) DNA in Environmental Samples

Western North American Naturalist ◽

10.3398/064.078.0109 ◽

2018 ◽

Vol 78 (1) ◽

pp. 92 ◽

Cited By ~ 1

Author(s):

Joseph C. Dysthe ◽

Kellie J. Carim ◽

Thomas W. Franklin ◽

Dave Kikkert ◽

Michael K. Young ◽

...

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Leatherside Chub

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Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Antibiotics ◽

10.3390/antibiotics9110787 ◽

2020 ◽

Vol 9 (11) ◽

pp. 787

Author(s):

Yunxia Luan ◽

Nan Wang ◽

Cheng Li ◽

Xiaojun Guo ◽

Anxiang Lu

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Electrochemical Sensors ◽

Detection Method ◽

Aminoglycoside Antibiotics ◽

Future Research ◽

Social Concern ◽

Food Items ◽

Detection Technology ◽

Animal Medicine

Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

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Modeling valley fever (coccidioidomycosis) incidence on the basis of climate conditions

International Journal of Biometeorology ◽

10.1007/s00484-002-0155-x ◽

2003 ◽

Vol 47 (2) ◽

pp. 87-101 ◽

Cited By ~ 48

Author(s):

Korine N. Kolivras ◽

Andrew C. Comrie

Keyword(s):

Valley Fever ◽

Climate Conditions

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Molecular Detection of Group C Rotavirus in Environmental Samples in Giza, Egypt

Asian Journal of Water Environment and Pollution ◽

10.3233/ajw190044 ◽

2019 ◽

Vol 16 (4) ◽

pp. 17-22

Author(s):

Mohamed N.F. Shaheen ◽

Elmahdy M. Elmahdy

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Group C Rotavirus

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Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

Advances in Oceanography and Limnology ◽

10.4081/aiol.2017.6394 ◽

2017 ◽

Vol 8 (1) ◽

Cited By ~ 4

Author(s):

Latife Köker ◽

Reyhan Akçaalan ◽

Meriç Albay ◽

Brett A. Neilan

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Freshwater Ecosystems ◽

Molecular Methods ◽

Marmara Region ◽

Microcystin Synthetase ◽

Toxic Cyanobacteria ◽

Mcye Gene ◽

Microcystin Production ◽

Inland Water Bodies

Blooms of cyanobacteria are an increasingly frequent phenomenon in freshwater ecosystems worldwide as a result of eutrophication. Many species can produce hepatotoxins that cause severe health hazards to humans. The aim of this study was to identify the bloom forming cyanobacteria species by molecular methods and to amplify genes responsible for hepatotoxin biosynthesis from the environmental samples and isolated strains of cyanobacteria from Küçükçekmece Lagoon, Sapanca, İznik, Manyas and Taşkısı Lakes. A total of 10 bloom samples and 11 isolated strains were examined and Microcystis spp., Planktothrix spp., Nodularia spumigena, Anabaenopsis elenkinii, Sphaerospermopsis aphanizomenoides, Cylindrospermopsis raciborskii were identified. Hepatotoxin genes were detected in 60% of the bloom samples and 45% of the strains. Two Microcystis strains were obtained from Küçükçekmece Lagoon. While the strain assigned to Microcystis flosaquae was non-toxic, Microcystis aeruginosa strain produced microcystin. According to PCR results, the M. aeruginosa and Planktothrix agardhii bloom samples of Küçükçekmece Lagoon contained the microcystin synthetase gene E (mcyE) indicative of microcystin production, however, no microcystin was detected by HPLC. The mcyE gene was also found in Microcystis wesenbergii isolated from Taşkısı Lake, and in all Planktothrix rubescens bloom samples from Sapanca Lake. To our knowledge, this is the first detailed study for identifiying different toxic cyanobacteria species and their hepatotoxin production from several waterbodies in Turkey using molecular methods.

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