Molecular Detection of Northern Leatherside Chub (Lepidomeda copei) DNA in Environmental Samples

ABSTRACT Ratiometric pre-rRNA analysis (RPA) detects the replenishment of rRNA precursors that occurs rapidly upon nutritional stimulation of bacterial cells. In contrast to DNA detection by PCR, RPA distinguishes viable from inactivated bacteria. It exhibits promise as a molecular viability test for pathogens in water and other environmental samples.

Download Full-text

Advances in the Application of Aptamer Biosensors to the Detection of Aminoglycoside Antibiotics

Antibiotics ◽

10.3390/antibiotics9110787 ◽

2020 ◽

Vol 9 (11) ◽

pp. 787

Author(s):

Yunxia Luan ◽

Nan Wang ◽

Cheng Li ◽

Xiaojun Guo ◽

Anxiang Lu

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Electrochemical Sensors ◽

Detection Method ◽

Aminoglycoside Antibiotics ◽

Future Research ◽

Social Concern ◽

Food Items ◽

Detection Technology ◽

Animal Medicine

Antibiotic abuse is becoming increasingly serious and the potential for harm to human health and the environment has aroused widespread social concern. Aminoglycoside antibiotics (AGs) are broad-spectrum antibiotics that have been widely used in clinical and animal medicine. Consequently, their residues are commonly found in animal-derived food items and the environment. A simple, rapid, and sensitive detection method for on-site screening and detection of AGs is urgently required. In recent years, with the development of molecular detection technology, nucleic acid aptamers have been successfully used as recognition molecules for the identification and detection of AGs in food and the environment. These aptamers have high affinities, selectivities, and specificities, are inexpensive, and can be produced with small batch-to-batch differences. This paper reviews the applications of aptamers for AG detection in colorimetric, fluorescent, chemiluminescent, surface plasmon resonance, and electrochemical sensors for the analysis in food and environmental samples. This study provides useful references for future research.

Download Full-text

Molecular Detection of Group C Rotavirus in Environmental Samples in Giza, Egypt

Asian Journal of Water Environment and Pollution ◽

10.3233/ajw190044 ◽

2019 ◽

Vol 16 (4) ◽

pp. 17-22

Author(s):

Mohamed N.F. Shaheen ◽

Elmahdy M. Elmahdy

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Group C Rotavirus

Download Full-text

Molecular detection of hepatotoxic cyanobacteria in inland water bodies of the Marmara Region, Turkey

Advances in Oceanography and Limnology ◽

10.4081/aiol.2017.6394 ◽

2017 ◽

Vol 8 (1) ◽

Cited By ~ 4

Author(s):

Latife Köker ◽

Reyhan Akçaalan ◽

Meriç Albay ◽

Brett A. Neilan

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Freshwater Ecosystems ◽

Molecular Methods ◽

Marmara Region ◽

Microcystin Synthetase ◽

Toxic Cyanobacteria ◽

Mcye Gene ◽

Microcystin Production ◽

Inland Water Bodies

Blooms of cyanobacteria are an increasingly frequent phenomenon in freshwater ecosystems worldwide as a result of eutrophication. Many species can produce hepatotoxins that cause severe health hazards to humans. The aim of this study was to identify the bloom forming cyanobacteria species by molecular methods and to amplify genes responsible for hepatotoxin biosynthesis from the environmental samples and isolated strains of cyanobacteria from Küçükçekmece Lagoon, Sapanca, İznik, Manyas and Taşkısı Lakes. A total of 10 bloom samples and 11 isolated strains were examined and Microcystis spp., Planktothrix spp., Nodularia spumigena, Anabaenopsis elenkinii, Sphaerospermopsis aphanizomenoides, Cylindrospermopsis raciborskii were identified. Hepatotoxin genes were detected in 60% of the bloom samples and 45% of the strains. Two Microcystis strains were obtained from Küçükçekmece Lagoon. While the strain assigned to Microcystis flosaquae was non-toxic, Microcystis aeruginosa strain produced microcystin. According to PCR results, the M. aeruginosa and Planktothrix agardhii bloom samples of Küçükçekmece Lagoon contained the microcystin synthetase gene E (mcyE) indicative of microcystin production, however, no microcystin was detected by HPLC. The mcyE gene was also found in Microcystis wesenbergii isolated from Taşkısı Lake, and in all Planktothrix rubescens bloom samples from Sapanca Lake. To our knowledge, this is the first detailed study for identifiying different toxic cyanobacteria species and their hepatotoxin production from several waterbodies in Turkey using molecular methods.

Download Full-text

Molecular detection of Leptospira interrogans in human tissues and environmental samples in a lethal case of leptospirosis

International Journal of Legal Medicine ◽

10.1007/s00414-007-0212-4 ◽

2007 ◽

Vol 122 (3) ◽

pp. 229-233 ◽

Cited By ~ 10

Author(s):

D. Luchini ◽

F. Meacci ◽

M. R. Oggioni ◽

G. Morabito ◽

V. D’Amato ◽

...

Keyword(s):

Environmental Samples ◽

Molecular Detection ◽

Leptospira Interrogans ◽

Human Tissues

Download Full-text

Improved Molecular Detection of Angiostrongylus cantonensis in Mollusks and Other Environmental Samples with a Species-Specific Internal Transcribed Spacer 1-Based TaqMan Assay

Applied and Environmental Microbiology ◽

10.1128/aem.00546-10 ◽

2010 ◽

Vol 76 (15) ◽

pp. 5287-5289 ◽

Cited By ~ 51

Author(s):

Yvonne Qvarnstrom ◽

Ana Cristina Aramburu da Silva ◽

John L. Teem ◽

Robert Hollingsworth ◽

Henry Bishop ◽

...

Keyword(s):

Internal Transcribed Spacer ◽

Environmental Samples ◽

Molecular Detection ◽

Angiostrongylus Cantonensis ◽

Taqman Assay ◽

Eosinophilic Meningitis ◽

Pcr Assay ◽

Food Items ◽

Third Stage ◽

Species Specific

ABSTRACT Angiostrongylus cantonensis is the most common cause of human eosinophilic meningitis. Humans become infected by ingesting food items contaminated with third-stage larvae that develop in mollusks. We report the development of a real-time PCR assay for the species-specific identification of A. cantonensis in mollusk tissue.

Download Full-text

Evaluation of 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes for the Detection of Listeria monocytogenes in Selected Foods and Environmental Surfaces: Collaborative Study, First Action 2014.07

Journal of AOAC International ◽

10.5740/jaoacint.15-031 ◽

2015 ◽

Vol 98 (4) ◽

pp. 980-992

Author(s):

Patrick Bird ◽

Jonathan Flannery ◽

Erin Crowley ◽

James Agin ◽

David Goins ◽

...

Keyword(s):

Listeria Monocytogenes ◽

Environmental Samples ◽

Molecular Detection ◽

Collaborative Study ◽

Probability Of Detection ◽

Cottage Cheese ◽

Inoculum Level ◽

Test Portion ◽

Detection Assay ◽

Stressed Cells

Abstract The 3M™ Molecular Detection Assay (MDA) Listeria monocytogenes combines isothermal amplification and bioluminescence to detect Listeria monocytogenes with high specificity and efficiency in select foods and environmental samples. The 3M MDA Listeria monocytogenes method was evaluated using an unpaired study design in a multilaboratory collaborative study to the U.S. Department of Agriculture, Food Safety and Inspection Service Microbiology Laboratory Guidebook 8.09 (2011) Isolation and Identification of Listeria monocytogenes from Red Meat, Poultry, and Egg Products and Environmental Samples for deli turkey, and the AOAC Official Method of AnalysisSM 993.12 Listeria monocytogenes in Milk and Dairy Products for full-fat (4% milk fat) cottage cheese following the current AOAC guidelines. A total of 16 laboratories located in the continental United States and Canada participated in this collaborative study. For deli turkey, 125 g test portions were evaluated using heat-stressed cells by each method. For full-fat cottage cheese, 25 g test portions were evaluated using nonheat-stressed cells. Each matrix had three inoculation levels: an uninoculated control level (0 CFU/test portion), and two levels artificially contaminated with L. monocytogenes, a low inoculum level (0.2–2 CFU/test portion) and a high inoculum level (2–5 CFU/test portion). In total, 1584 unpaired replicate samples were analyzed. Statistical analysis was conducted according to the probability of detection (POD) model. Results obtained for the low inoculum level full-fat cottage cheese test portions produced a difference in cross-laboratory POD (dLPOD) value of –0.08 with a 95% confidence interval (CI) of (–0.20, 0.05). For the low-level deli turkey test portions, a dLPOD value of –0.02 with a 95% CI of (–0.14, 0.11) was obtained.

Download Full-text