To provide a scientific basis and technical support for agroforestry management practices, such as interrow configuration and soil water and fertilizer management, a stratified excavation method was performed both to explore the fine-root spatial distribution and niche differentiation and to quantify the below-ground interspecific competition status of 3-, 5-, and 7-year-old apple (Malus pumila M.)–soybean (Glycine max L. Merr.) intercropping systems and monocropping systems. The fine roots of older trees occupied a larger soil space and had both a greater fine-root biomass density (FRMD) and a greater ability to reduce the FRMD of soybean, but this ability decreased with the distance from the apple tree row. Similarly, the FRMD of apple trees was also adversely affected by soybean plants, but this effect gradually increased with a decrease in tree age or with the distance from the tree row. Compared with that of the 3- and 5-year-old monocropped apple trees, the FRMD of the 3- and 5-year-old intercropped apple trees increased in the 40–100 cm and 60–100 cm soil layers, respectively. However, compared with that of the 7-year-old apple and soybean monocropping systems, the FRMD of the 7-year-old intercropped apple trees and soybean plants decreased in each soil layer. Compared with that of the corresponding monocropped systems, the fine-root vertical barycenter (FRVB) of the intercropped apple trees displaced deeper soil and that of the intercropped soybean plants displaced shallower soil. Furthermore, the FRVB of both intercropped apple trees and intercropped soybean plants displaced shallower soil with increasing tree age. Intense below-ground interspecific competition in the 3-, 5-, and 7-year-old apple–soybean intercropping systems occurred in the 0–40 cm soil layer at distances of 0.5–0.9, 0.5–1.3, and 0.5–1.7 m from the apple tree row, respectively.
Below-ground fine-scale distribution and soil versus fine root detection of fungal and soil oomycete communities in a French beech forest
